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Vol. 62, Issue 3, 439-445, September 2002

ACCELERATED COMMUNICATION
Development of A Real-Time in Vivo Transcription Assay: Application Reveals Pregnane X Receptor-Mediated Induction of CYP3A4 by Cancer Chemotherapeutic Agents

Erin Schuetz, Lubin Lan, Kazuto Yasuda, Richard Kim, Thomas A. Kocarek, John Schuetz, and Stephen Strom

Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, Tennessee (E.S., L.L., K.Y., J.S.); Department of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee (R.K.); Institute of Environmental Health Sciences, Wayne State University, Detroit, Michigan (T.A.K.); and Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania (S.S.)

We report the development of a rapid real-time assay that measures the transcription of luciferase reporter genes in transduced mouse hepatic cells in vivo. Luciferase activity is noninvasively measured by whole-body optical imaging within hours of the hydrodynamic injection of as little as 1 µg of naked DNA. Transcription of genes introduced as linearized DNA can be serially assayed for weeks in each animal. Transcription was quantified by extracorporal monitoring of bioluminescence as well as or better than by traditional in vitro bioluminescence assay. Our assay allows the measurement of transcription as it occurs, under the most informative biological conditions (i.e., in a living, intact organ). Furthermore, it substantially reduces the cost, time, and number of animals required for analysis of gene expression. The utility of the method is demonstrated in the discovery that topotecan and etoposide are ligands of pregnane X receptor that induce CYP3A4 transcription.

Copyright © 2002 by The American Society for Pharmacology and Experimental Therapeutics


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Copyright © 2002 by the American Society for Pharmacology and Experimental Therapeutics