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Vol. 62, Issue 3, 618-627, September 2002
Department of Pharmacology, University of Pittsburgh, Pittsburgh,
Pennsylvania
The emergence of zinc as a potent neurotoxin has prompted the
development of techniques suitable for the measurement of intracellular free zinc ([Zn2+]i) in cultured cells.
Accordingly, a new family of Zn2+-sensitive fluorophores
has become available. Using ionophore-induced elevations of
[Zn2+]i in cultured neurons, we measured
[Zn2+]i-induced changes in the novel dyes
FuraZin-1 and FluoZin-2 and compared them with the established
[Zn2+]i-sensitive fluorophores mag-fura-2 and
Newport Green. All of these dyes effectively detected
[Zn2+]i, and FuraZin-1, FluoZin-2, and
Newport Green showed selectivity for [Zn2+]i
over [Ca2+]i and
[Mg2+]i. However, the dyes showed little
difference in their apparent sensitivity to
[Zn2+]i, even though their in vitro
affinities for Zn2+ varied from 20 nM to 3 µM. We show
herein that this is a consequence of the relatively high concentrations
of intracellular dye used in experiments of this nature. Thus, for the
measurement of [Zn2+]i, the sensitivity of
the reporting system is dominated by the intracellular dye
concentration, whereas dye affinity is unimportant. We extend these
findings to show that calibration of dye signal to ion concentration is
critically dependent on precise measurement of intracellular dye concentration.
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