![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 63, Issue 3, 478-488, March 2003
12,
G13, Gq, and G
for
Endothelin-1-Induced c-Jun NH2-Terminal Kinase and
Extracellular Signal-Regulated Kinase Activation
Laboratory of Pharmacology and Toxicology, Graduate School
of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan (K.A.,
Y.M., M.N., S.Tan., S.Tak., T.N., H.K.); Division of Molecular and
Cellular Physiology, Center for Integrative Bioscience, National
Institute for Physiological Sciences, Okazaki, Japan (M.N., Y.,M.); and
Department of Pharmacology, University of Illinois at Chicago, Chicago,
Illinois (T.K.)
In the present study, we examined the roles of G12,
G13, Gq, and Gi in
endothelin-1-induced hypertrophic responses. Endothelin-1 stimulation
activated extracellular signal-regulated kinase (ERK) and c-Jun
NH2-terminal kinase (JNK) in cultured rat neonatal
myocytes. The activation of JNK, but not ERK, was inhibited by the
expression of carboxyl terminal regions of G
12 and
G13. JNK activation was also inhibited by expression of
the G12/G13-specific inhibitor regulator
of G protein signaling (RGS) domain of p115RhoGEF and the
Gq-specific inhibitor RGS domain of the G
protein-coupled receptor kinase 2 (GRK2-RGS). JNK activation was not,
however, inhibited by expression of the carboxyl terminal region of G
protein-coupled receptor kinase 2 (GRK2-ct), which is a
G
-sequestering polypeptide. Additionally, JNK activation but not
ERK activation was inhibited by the expression of C3 exoenzyme that
inactivates small GTPase Rho. These results suggest that JNK activation
by G12, G13, and Gq is
involved in Rho. On the other hand, ERK activation was inhibited by
pertussis toxin treatment, the receptor-Gi uncoupler, and
GRK2-ct. Thus, ERK was activated by Gi- and
G-dependent pathways. These results clearly demonstrate that
differential pathways activate JNK and ERK.
This article has been cited by other articles:
|
|
 |
|
  F. Lin, S. Chen, D. S. Sepich, J. R. Panizzi, S. G. Clendenon, J. A. Marrs, H. E. Hamm, and L. Solnica-Krezel G{alpha}12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton J. Cell Biol., March 23, 2009; 184(6): 909 - 921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Komachi, H. Tomura, E. Malchinkhuu, M. Tobo, C. Mogi, T. Yamada, T. Kimura, A. Kuwabara, H. Ohta, D.-S. Im, et al. LPA1 receptors mediate stimulation, whereas LPA2 receptors mediate inhibition, of migration of pancreatic cancer cells in response to lysophosphatidic acid and malignant ascites Carcinogenesis, March 1, 2009; 30(3): 457 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kimura, C. Mogi, H. Tomura, A. Kuwabara, D.-S. Im, K. Sato, H. Kurose, M. Murakami, and F. Okajima Induction of Scavenger Receptor Class B Type I Is Critical for Simvastatin Enhancement of High-Density Lipoprotein-Induced Anti-Inflammatory Actions in Endothelial Cells J. Immunol., November 15, 2008; 181(10): 7332 - 7340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Iguchi, K. Sakata, K. Yoshizaki, K. Tago, N. Mizuno, and H. Itoh Orphan G Protein-coupled Receptor GPR56 Regulates Neural Progenitor Cell Migration via a G{alpha}12/13 and Rho Pathway J. Biol. Chem., May 23, 2008; 283(21): 14469 - 14478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Kimple, J. W. Joseph, C. L. Bailey, P. T. Fueger, I. A. Hendry, C. B. Newgard, and P. J. Casey G{alpha}z Negatively Regulates Insulin Secretion and Glucose Clearance J. Biol. Chem., February 22, 2008; 283(8): 4560 - 4567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nishida, N. Onohara, Y. Sato, R. Suda, M. Ogushi, S. Tanabe, R. Inoue, Y. Mori, and H. Kurose G{alpha}12/13-mediated Up-regulation of TRPC6 Negatively Regulates Endothelin-1-induced Cardiac Myofibroblast Formation and Collagen Synthesis through Nuclear Factor of Activated T Cells Activation J. Biol. Chem., August 10, 2007; 282(32): 23117 - 23128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Koike, H. Obinata, A. Yamamoto, S. Takeda, H. Komori, F. Nara, T. Izumi, and T. Haga 5-Oxo-Eicosatetraenoic Acid-Induced Chemotaxis: Identification of a Responsible Receptor hGPCR48 and Negative Regulation by G Protein G12/13. J. Biochem., March 1, 2006; 139(3): 543 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Kimple, A. B. Nixon, P. Kelly, C. L. Bailey, K. H. Young, T. A. Fields, and P. J. Casey A Role for Gz in Pancreatic Islet {beta}-Cell Biology J. Biol. Chem., September 9, 2005; 280(36): 31708 - 31713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Obara, H. Kurose, and N. Nakahata Thromboxane A2 Promotes Interleukin-6 Biosynthesis Mediated by an Activation of Cyclic AMP-Response Element-Binding Protein in 1321N1 Human Astrocytoma Cells Mol. Pharmacol., September 1, 2005; 68(3): 670 - 679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Maeda, I. Matsuoka, T. Iwamoto, H. Kurose, and J. Kimura Down-Regulation of Na+/Ca2+ Exchanger by Fluvastatin in Rat Cardiomyoblast H9c2 Cells: Involvement of RhoB in Na+/Ca2+ Exchanger mRNA Stability Mol. Pharmacol., August 1, 2005; 68(2): 414 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fujii, N. Onohara, Y. Maruyama, S. Tanabe, H. Kobayashi, M. Fukutomi, Y. Nagamatsu, N. Nishihara, R. Inoue, H. Sumimoto, et al. G{alpha}12/13-mediated Production of Reactive Oxygen Species Is Critical for Angiotensin Receptor-induced NFAT Activation in Cardiac Fibroblasts J. Biol. Chem., June 17, 2005; 280(24): 23041 - 23047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lin, D. S. Sepich, S. Chen, J. Topczewski, C. Yin, L. Solnica-Krezel, and H. Hamm Essential roles of G{alpha}12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements J. Cell Biol., June 6, 2005; 169(5): 777 - 787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nishida, S. Tanabe, Y. Maruyama, S. Mangmool, K. Urayama, Y. Nagamatsu, S. Takagahara, J. H. Turner, T. Kozasa, H. Kobayashi, et al. G{alpha}12/13- and Reactive Oxygen Species-dependent Activation of c-Jun NH2-terminal Kinase and p38 Mitogen-activated Protein Kinase by Angiotensin Receptor Stimulation in Rat Neonatal Cardiomyocytes J. Biol. Chem., May 6, 2005; 280(18): 18434 - 18441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-N. Lee, C. C. Malbon, and H.-y. Wang G{alpha}13 Signals via p115RhoGEF Cascades Regulating JNK1 and Primitive Endoderm Formation J. Biol. Chem., December 24, 2004; 279(52): 54896 - 54904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. McNeil Coffield, W. S. Helms, Q. Jiang, and L. Su G{alpha}13 Mediates a Signal That Is Essential for Proliferation and Survival of Thymocyte Progenitors J. Exp. Med., November 15, 2004; 200(10): 1315 - 1324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lahlou, N. Saint-Laurent, J.-P. Esteve, A. Eychene, L. Pradayrol, S. Pyronnet, and C. Susini sst2 Somatostatin Receptor Inhibits Cell Proliferation through Ras-, Rap1-, and B-Raf-dependent ERK2 Activation J. Biol. Chem., October 10, 2003; 278(41): 39356 - 39371. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
