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Tiotidine, a Histamine H2 Receptor Inverse Agonist That Binds with High Affinity to an Inactive G-Protein—Coupled Form of the Receptor. Experimental Support for the Cubic Ternary Complex Model

Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica (F.M., N.F., B.L.L., M.E.R., C.D.) and Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales (C.S.), Universidad de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina (F.M., N.F., A.B., C.S., C.D.); and Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina (N.F., A.B., C.S.)

Knowing the importance for research and pharmacological uses of proper ligand classification into agonists, inverse agonists, and antagonists, the aim of this work was to study the behavior of tiotidine, a controversial histamine H2 receptor ligand. We found that tiotidine, described previously as an H2 antagonist, actually behaves as an inverse agonist in U-937 cells, diminishing basal cAMP levels. [³H]Tiotidine showed two binding sites, one with high affinity and low capacity and the other with low affinity and high capacity. The former site disappeared in the presence of guanosine 5'-O-(3-thio)triphosphate, indicating that it belongs to a subset of receptors coupled to G-protein, showing the classic binding profile for an agonist. Considering the occupancy models developed up to now, the only one that explains tiotidine dual behavior is the cubic ternary complex (CTC) model. This model allows G-protein to interact with the receptor even in the inactive state. We showed by theoretical simulations based on the CTC model of dose-response and binding experiments that tiotidine biases the system to a G-protein—coupled form of the receptor that is unable to evoke a response. This theoretical approach was supported by experimental results in which an unrelated G-protein—coupled receptor that also signals through G_s-protein (₂-adrenoreceptor) was impeded by tiotidine. This interference clearly implies that tiotidine biases the system to G_s-coupled form of the H2 receptor and turns G_s-protein less available to interact with ₂-adrenoreceptor. These findings not only show that tiotidine is an H2 inverse agonist in U-937 cells but also provide experimental support for the CTC model.

Address correspondence to: Dr. Davio Carlos, Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Junin 956 PB, 1113, Capital Federal, Argentina. E-mail: cardavio{at}ffyb.uba.ar

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