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0026-895X/03/6404-885-889$20.00
Mol Pharmacol 64:885-889, 2003

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Functional Nicotinic Acetylcholine Receptors Are Expressed in B Lymphocyte-Derived Cell Lines

Marina V. Skok, Elena N. Kalashnik, Ludmila N. Koval, Victor I. Tsetlin, Yuriy N. Utkin, Jean-Pierre Changeux, and Régis Grailhe

Palladin Institute of Biochemistry, Kiev, Ukraine (M.V.S., E.N.K., L.N.K.); Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia (V.I.T., Y.N.U.); and Institut Pasteur, Paris, France (J.P.C., R.G.)

Nicotine has been shown to affect B lymphocyte immune response. In this study, we have explored the presence of nicotinic receptors in B lymphocyte-derived cell lines, myeloma X63-Ag8 and hybridoma 1D6. We found that myeloma expressed on average 10,170 ± 1,100 [3H]epibatidine and 6,730 ± 370 125I-{alpha}-bungarotoxin binding sites per cell, thus reflecting the presence of both homomeric and heteromeric nicotinic receptors. More specifically, the presence of {alpha}4- and {alpha}7-containing nicotinic receptor subunits was demonstrated in both myeloma and hybridoma cells with subunit-specific antibodies. It was significantly higher in dividing than in resting cells. Long-term exposure to nicotine, at physiological concentration found in smokers, resulted in up-regulation of both {alpha}4 and {alpha}7 subunits in hybridoma cells. Additionally, nicotine stimulated hybridoma cell proliferation, whereas it decreased antibody production. In contrast, {alpha}7-specific snake toxins inhibited cell proliferation but increased antibody production. It is concluded that myeloma and hybridoma cells express {alpha}4- and {alpha}7-containing nicotinic receptors, which participate in regulating cell proliferation and function.

Received April 15, 2003; accepted July 3, 2003

Address correspondence to: Dr. Marina V. Skok, Palladin Institute of Biochemistry, 9 Leontovicha Str., 01030 Kiev, Ukraine. E-mail: skok{at}biochem.kiev.ua




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