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Department of Physiology & Pharmacology, Oregon Health & Science University, Portland, Oregon (J.D.B., X.L., K.S., C.J.F., J.W.K., J.R.M.); Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston, Houston, Texas (T.C.R.); and Neurological Sciences Institute, Oregon Health & Science University, Beaverton, Oregon (L.L., R.L.B.)
Cyclic nucleotide-gated (CNG) channels are the primary targets of light- and odorant-induced signaling in photoreceptors and olfactory sensory neurons. Compartmentalized cyclic nucleotide signaling is necessary to ensure rapid and efficient activation of these nonselective cation channels. However, relatively little is known about the subcellular localization of CNG channels or the mechanisms of their membrane partitioning. Lipid raft domains are specialized membrane microdomains rich in cholesterol and sphingolipids that have been implicated in the organization of many membrane-associated signaling pathways. Herein, we report that the 
subunit of the olfactory CNG channel, CNGA2, associates with lipid rafts in heterologous expression systems and in rat olfactory epithelium. However, CNGA2 does not directly bind caveolin, and its membrane localization overlaps only slightly with that of caveolin at the surface of human embryonic kidney (HEK) 293 cells. To test for a possible functional role of lipid raft association, we treated HEK 293 cells with the cholesterol-depleting agent, methyl-
-cyclodextrin. Cholesterol depletion abolished prostaglandin E1-stimulated CNGA2 channel activity in intact cells. Recordings from membrane patches excised from CNGA2-expressing HEK 293 cells revealed that cholesterol depletion dramatically reduced the apparent affinity of homomeric CNGA2 channels for cAMP but only slightly reduced the maximal current. Our results show that olfactory CNG channels target to lipid rafts and that disruption of lipid raft microdomains dramatically alters the function of CNGA2 channels.
Address correspondence to: Jeffrey R. Martens, Dept. of Physiology and Pharmacology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, L334, Portland, OR 97239-3098. E-mail: martensj{at}ohsu.edu
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