QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pae, H.-O. Articles by Chung, H.-T. Search for Related Content PubMed PubMed Citation Articles by Pae, H.-O. Articles by Chung, H.-T.

Roles of Heme Oxygenase-1 in the Antiproliferative and Antiapoptotic Effects of Nitric Oxide on Jurkat T Cells

Department of Microbiology and Immunology, Wonkwang University School of Medicine (H.O.P., B.M.C., G.S.O., M.S.L., H.T.C.), and Department of Physiology, Wonkwang University Oriental Medicine (D.G.R.), Iksan, Republic of Korea; Department of Urology, Kosin University Hospital, Busan, Republic of Korea (H.Y.R.); and Department of Molecular and Cellular Biochemistry, Kangwon National University School of Medicine, Chunchon, Republic of Korea (Y.M.K)

Nitric oxide (NO) has been shown to exert antiproliferative and antiapoptotic effects on human T cells. Heme oxygenase-1 (HO-1), which degrades heme into biliverdin, free iron (Fe²⁺), and carbon monoxide (CO), has also been known to have antiproliferative and antiapoptotic effects. Recent evidence suggests that HO-1 is an important cellular target of NO; whether HO-1 expression contributes to the antiproliferative and/or antiapoptotic effects mediated by NO remains to be investigated. In the present study, we examined the effects of NO on HO-1 expression and possible roles of HO-1 in T cell proliferation and apoptosis. Using human Jurkat T cells, we found that the NO donor sodium nitroprusside (SNP) induced HO-1 expression and that preincubation with SNP suppressed T cell proliferation induced by concanavalin A and apoptosis triggered by anti-Fas antibody. Suppressions of T cell proliferation and apoptosis comparable with SNP were also observed when the T cells were preincubated with the HO-1 inducer cobalt protoporphyrin. A phosphorothioate-linked HO-1 antisense oligonucleotide blocked HO-1 expression, and subsequently abrogated the antiproliferative and antiapoptotic effects of SNP. Overexpression of the HO-1 gene after transfection into Jurkat T cells resulted in significant decreases in T cell proliferation and apoptosis. The CO donor tricarbonyldichlororuthenium (II) dimer mimicked the antiproliferative effect of SNP, and the Fe²⁺ donor FeSO₄ blocked anti-Fas-induced apoptosis. Taken together, our results suggest that NO induces HO-1 expression in T cells and that suppressions of T cell proliferation and apoptosis afforded by NO are associated with an increased expression of HO-1 by NO.

Address correspondence to: Hun-Taeg Chung, Department of Microbiology and Immunology, Wonkwang University Medical School, 344-2 Shinyong-Dong, Iksan, Chonbug 570-749, Republic of Korea. E-mail: htchung{at}wonkwang.ac.kr

This article has been cited by other articles:

				M L Ferrandiz, N Maicas, I Garcia-Arnandis, M C Terencio, R Motterlini, I Devesa, L A B Joosten, W B van den Berg, and M J Alcaraz Treatment with a CO-releasing molecule (CORM-3) reduces joint inflammation and erosion in murine collagen-induced arthritis Ann Rheum Dis, September 1, 2008; 67(9): 1211 - 1217. [Abstract] [Full Text] [PDF]

				C.-M. Hu, H.-H. Lin, M.-T. Chiang, P.-F. Chang, and L.-Y. Chau Systemic Expression of Heme Oxygenase-1 Ameliorates Type 1 Diabetes in NOD Mice Diabetes, May 1, 2007; 56(5): 1240 - 1247. [Abstract] [Full Text] [PDF]

				P. Chanvorachote, U. Nimmannit, L. Wang, C. Stehlik, B. Lu, N. Azad, and Y. Rojanasakul Nitric Oxide Negatively Regulates Fas CD95-induced Apoptosis through Inhibition of Ubiquitin-Proteasome-mediated Degradation of FLICE Inhibitory Protein J. Biol. Chem., December 23, 2005; 280(51): 42044 - 42050. [Abstract] [Full Text] [PDF]

				S. B. Blumenthal, A. K. Kiemer, G. Tiegs, S. Seyfried, M. Holtje, B. Brandt, H.-D. Holtje, S. Zahler, and A. M. Vollmar Metalloporphyrins inactivate caspase-3 and -8 FASEB J, August 1, 2005; 19(10): 1272 - 1279. [Abstract] [Full Text] [PDF]

				H.-O. Pae, G.-S. Oh, B.-M. Choi, Y.-M. Kim, and H.-T. Chung A Molecular Cascade Showing Nitric Oxide-Heme Oxygenase-1-Vascular Endothelial Growth Factor-Interleukin-8 Sequence in Human Endothelial Cells Endocrinology, May 1, 2005; 146(5): 2229 - 2238. [Abstract] [Full Text] [PDF]

				T. M. Brusko, C. H. Wasserfall, A. Agarwal, M. H. Kapturczak, and M. A. Atkinson An Integral Role for Heme Oxygenase-1 and Carbon Monoxide in Maintaining Peripheral Tolerance by CD4+CD25+ Regulatory T Cells J. Immunol., May 1, 2005; 174(9): 5181 - 5186. [Abstract] [Full Text] [PDF]