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Role of Liver-Enriched Transcription Factors in the Down-Regulation of Organic Anion Transporting Polypeptide 4 (Oatp4; Oatplb2; Slc21a10) by Lipopolysaccharide

Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas

Lipopolysaccharide (LPS) administration is a model of cholestasis. Organic anion transporting polypeptide 4 (Oatp4; Slc21a10) is almost exclusively expressed in liver. Therefore, it was hypothesized that LPS would down-regulate mouse Oatp4 and that this action is due to a decrease in nuclear binding activity of one or more liver-enriched transcription factors to mouse Oatp4 promoter. The present study indicates a time-dependent decrease in mouse Oatp4 mRNA levels by LPS. Moreover, LPS produced a rapid and profound decrease in nuclear binding activity to the mouse Oatp4 putative response elements for hepatocyte nuclear factor (HNF) 1, CAAT/enhancer binding protein (C/EBP), HNF3, and heterodimers of retinoid X receptor (RXR) and retinoic acid receptor (RAR). Maximal decrease in nuclear binding activity to these response elements preceded a significant reduction of Oatp4 mRNA levels. HNF1 bound to the Oatp4 HNF1 response element as a homodimer. Multiple copies of the Oatp4 HNF1 response element, inserted upstream of a minimal promoter, were sufficient to mediate reporter activity and responded to the coexpression of HNF1 in mouse hepatoma cells. Moreover, HNF1 dose dependently activated the Oatp4 promoter (–4.8 kilo-bases to +30 bp). Therefore, HNF1 is a potent trans-activator of the mouse Oatp4 promoter. In addition, Oatp4 mRNA levels were markedly decreased (95%) in HNF1-null mice as compared with wild-type mice, suggesting that HNF1 levels are critical for the constitutive expression of the Oatp4 gene. Taken together, these findings suggest that the LPS-induced down-regulation of Oatp4 is likely due to reduction in the binding of HNF1, C/EBP, HNF3, and RXR:RAR to the Oatp4 promoter.

Address correspondence to: Dr. Curtis D. Klaassen, Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160. E-mail: cklaasse{at}kumc.edu

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