QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: mol.104.004325v1 68/1/145    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sakai, H. Articles by Misawa, M. Search for Related Content PubMed PubMed Citation Articles by Sakai, H. Articles by Misawa, M.

Possible Involvement of CPI-17 in Augmented Bronchial Smooth Muscle Contraction in Antigen-Induced Airway Hyper-Responsive Rats

Department of Pharmacology, School of Pharmacy, Hoshi University, Tokyo, Japan

Airway hyper-responsiveness (AHR) associated with heightened airway resistance and inflammation is a characteristic feature of asthma. It has been demonstrated that contractile responsiveness and Ca²⁺ sensitization to acetylcholine (ACh) in repeated antigen challenge-induced airway hyper-responsive bronchial preparation were significantly increased. The CPI-17 (PKC-potentiated inhibitory protein for heterotrimeric myosin light chain phosphatase of 17 kDa) is activated by protein kinase C and acts on a myosin light-chain phosphatase-specific target. The aim of the present study was to explore the role of CPI-17 in hyper-responsiveness of bronchial smooth muscle in antigen-induced AHR rats. In immunoblotting, the levels of expression of CPI-17 mRNA and protein were significantly increased in bronchus from rats that were repeatedly challenged with antigen. ACh-induced CPI-17 phosphorylation and translocation to membrane fraction were also significantly increased in bronchus from antigen-challenged rats. In conclusion, we suggest that augmented expression and activation of CPI-17 observed in the hyper-responsive bronchial smooth muscle might be responsible for the enhanced ACh-induced Ca²⁺ sensitization of bronchial smooth muscle contraction associated with AHR.

Address correspondence to: Hiroyasu Sakai, Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. E-mail: sakai{at}hoshi.ac.jp

This article has been cited by other articles:

				C. Morin, M. Sirois, V. Echave, E. Rizcallah, and E. Rousseau Relaxing effects of 17(18)-EpETE on arterial and airway smooth muscles in human lung Am J Physiol Lung Cell Mol Physiol, January 1, 2009; 296(1): L130 - L139. [Abstract] [Full Text] [PDF]

				C. Morin, M. Sirois, V. Echave, and E. Rousseau CPI-17 Silencing-Reduced Responsiveness in Control and TNF-{alpha}-Treated Human Bronchi Am. J. Respir. Cell Mol. Biol., December 1, 2008; 39(6): 638 - 643. [Abstract] [Full Text] [PDF]

				Y. Chiba, J. Arima, H. Sakai, and M. Misawa Lovastatin inhibits bronchial hyperresponsiveness by reducing RhoA signaling in rat allergic asthma Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L705 - L713. [Abstract] [Full Text] [PDF]

				C. Morin, C. Guibert, M. Sirois, V. Echave, M. M. Gomes, and E. Rousseau Effects of {omega}-hydroxylase product on distal human pulmonary arteries Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1435 - H1443. [Abstract] [Full Text] [PDF]

				C. Morin, M. Sirois, V. Echave, M. M. Gomes, and E. Rousseau EET Displays Anti-Inflammatory Effects in TNF-{alpha} Stimulated Human Bronchi: Putative Role of CPI-17 Am. J. Respir. Cell Mol. Biol., February 1, 2008; 38(2): 192 - 201. [Abstract] [Full Text] [PDF]

				C. Morin, M. Sirois, V. Echave, M. M. Gomes, and E. Rousseau Epoxyeicosatrienoic Acid Relaxing Effects Involve Ca2+-Activated K+ Channel Activation and CPI-17 Dephosphorylation in Human Bronchi Am. J. Respir. Cell Mol. Biol., May 1, 2007; 36(5): 633 - 641. [Abstract] [Full Text] [PDF]

				Y. Bai and M. J. Sanderson Modulation of the Ca2+ sensitivity of airway smooth muscle cells in murine lung slices Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L208 - L221. [Abstract] [Full Text] [PDF]

				Y. Chiba, M. Murata, H. Ushikubo, Y. Yoshikawa, A. Saitoh, H. Sakai, J. Kamei, and M. Misawa Effect of Cigarette Smoke Exposure In Vivo on Bronchial Smooth Muscle Contractility In Vitro in Rats Am. J. Respir. Cell Mol. Biol., December 1, 2005; 33(6): 574 - 581. [Abstract] [Full Text] [PDF]

				H. Sakai, T. Hirano, Y. Chiba, and M. Misawa Acetylcholine-induced phosphorylation and membrane translocation of CPI-17 in bronchial smooth muscle of rats Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L925 - L930. [Abstract] [Full Text] [PDF]