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Scavenger Receptors on Sinusoidal Liver Endothelial Cells Are Involved in the Uptake of Aldehyde-Modified Proteins

Departments of Internal Medicine (M.J.D., T.L.F., C.D.H., B.C.H., D.J.T., L.W.K., G.M.T.) and Pathology and Microbiology (G.M.T.), University of Nebraska Medical Center, Omaha, Nebraska; and Veterans Administration Alcohol Research Center, Omaha Veterans Administration Medical Center, Omaha, Nebraska (M.J.D., T.L.F., M.S., C.D.H., B.C.H., H.S., D.J.T., L.W.K., G.M.T.)

Scavenger receptors on sinusoidal liver endothelial cells (SECs) eliminate potentially harmful modified proteins circulating through the liver. It was shown recently that aldehyde-modified proteins bind to scavenger receptors and are associated with the development/progression of alcoholic liver diseases. For these studies, rat livers were perfused in situ with ¹²⁵I-formaldehyde-bovine serum albumin (f-Alb) or ¹²⁵I-malondialdehyde-acetaldehyde-bovine serum albumin (MAA-Alb) in the presence of known scavenger receptor ligands as inhibitors. Reverse transcription-polymerase chain reaction (RT-PCR) analysis and scavenger receptor Type A (SRA) knock-out mice were used to assess the role of these receptors in mediating immune responses. The degradation of ¹²⁵I-f-Alb or ¹²⁵I-MAA-Alb in whole livers and isolated SECs can be inhibited by known scavenger receptor ligands, including f-Alb, maleylated bovine albumin, and fucoidan. ¹²⁵I-f-Alb could not be completely inhibited by MAA-Alb. In contrast, ¹²⁵I-MAA-Alb was only partially inhibited with advanced glycosylated endproduct albumin. RT-PCR data show the presence of a number of scavenger receptors on SECs that may be responsible for the binding of MAA-modified proteins. SRA seems to be one of these receptors involved in the effects mediated by MAA-modified proteins. In a study using SRA knockout mice, it was shown that a decreased antibody response to MAA-Alb resulted. By RT-PCR, CD36, LOX-1, and SR-AI are the scavenger receptors most likely involved in the degradation of MAA-Alb.

Address correspondence to: Michael J. Duryee, Omaha VA Medical Center, Research Services 151, Room 325, 4101 Woolworth Avenue, Omaha, NE 68105. E-mail: mduryee{at}unmc.edu

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