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Stereospecific Induction of Nuclear Factor-B Activation by Isochamaejasmin

The National Center for Drug Screening, Shanghai Institute of Materia Medica (X.X., M.S., C.Z., T.A., L.H., M.-W.W.) and the Graduate School (Q.T.), Chinese Academy of Sciences, Shanghai, China; and Department of Pharmacology, College of Medicine, University of Illinois, Chicago, Illinois (J.L., H.S., R.D.Y.)

The root of Stellera chamaejasme L. is a traditional Chinese herb termed Rui Xiang Lang Du and has been used to treat solid tumors, tuberculosis and psoriasis. Exactly how S. chamaejasme L. regulates cellular responses remains unclear. We examined four biflavonoids isolated from S. chamaejasme L., including isochamaejasmin, two of its stereo-isomers and a methyl derivative, in functional assays originally designed to screen ligands for the G protein-coupled formyl peptide receptor-like 1 (FPRL1). Isochamaejasmin was found to induce the expression of a nuclear factor (NF)-B-directed reporter gene in transfected HeLa cells with an EC₅₀ of 3.23 µM, independently of FPRL1. The isochamaejasmin-stimulated NF-B reporter activity was accompanied by nuclear translocation of NF-B proteins and was blocked by a dominant-negative construct of IB. Isochamaejasmin also induced time-dependent phosphorylation of the mitogen-activated protein kinases extracellular signal-regulated kinase 1/2 and p38, and a novel protein kinase C (PKC). Likewise, inhibition of these kinases with the respective pharmacological inhibitors significantly reduced the isochamaejasmin-stimulated NF-B activation. It is noteworthy that the two stereoisomers and the methyl derivative did not induce detectable activation of NF-B and were more cytotoxic than isochamaejasmin, which could partially rescue cycloheximide-induced apoptosis. Inhibition of NF-B activation reversed the anti-apoptotic effect of isochamaejasmin. These results provide the first evidence for a potential mechanism of action by S. chamaejasme L., and indicate that structurally similar compounds derived from S. chamaejasme L. may have different pharmacological properties.

Address correspondence to: Ming-Wei Wang, the National Center for Drug Screening, 189 Guo Shou Jing Road, Shanghai 201203, China. E-mail: center{at}mail.shcnc.ac.cn

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