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Peroxisome Proliferator-Activated Receptor -Independent Repression of Prostate-Specific Antigen Expression by Thiazolidinediones in Prostate Cancer Cells

Division of Medicinal Chemistry, College of Pharmacy (C.-C.Y., C.-Y.K., S.W., C.-W.S., Cha.-S.C., Chi.-S.C.) and Divisions of Endocrinology and Oncology, Department of Internal Medicine (J.J.P., M.D.R.), The Ohio State University, Columbus, Ohio

In light of the potential use of the thiazolidinedione family of peroxisome proliferator-activated receptor- (PPAR) agonists in prostate cancer treatment, this study assessed the mechanism by which these agents suppress prostate-specific antigen (PSA) secretion in prostate cancer cells. Two lines of evidence indicate that the effect of thiazolidinediones on PSA down-regulation is independent of PPAR activation. First, this thiazolidinedione-mediated PSA down-regulation is structure-specific irrespective of the relative PPAR agonist potency. Second, the PPAR-inactive analogs of troglitazone and ciglitazone [2TG (5-[4-(6-hydroxy-2,5,7,8-tetramethyl-chroman-2-yl-methoxy)-benzylidene]-thiazolidine-2,4-dione) and 2CG (5-[4-(1-methyl-cyclohexylmethoxy)-benzylidene]-thiazolidine-2,4-dione), respectively] exhibit higher potency than the parent compound in inhibiting dihydrotestosterone (DHT)-stimulated PSA secretion. Although 10 µM troglitazone and 2TG significantly inhibit PSA secretion, they do not alter the expression level of androgen receptor (AR) or interfere with DHT-activated nuclear translocation of AR. However, reporter gene and chromatin immunoprecipitation studies indicate that troglitazone and 2TG block AR recruitment to the androgen response elements within the PSA promoter. Thus, this study raises the question of whether the ability of oral troglitazone to reduce PSA levels in prostate cancer patients is therapeutically relevant. A major concern is that the concentration for troglitazone to mediate antitumor effects is severalfold higher than that of PSA down-regulation, which is difficult to attain at therapeutic doses. Nevertheless, it is noteworthy that troglitazone and 2TG at high doses were able to inhibit AR expression. From a translational perspective, separation of PPAR agonist activity from AR down-regulation provides a molecular basis to use troglitazone as a platform to design AR-ablative agents.

Address correspondence to: Ching-Shih Chen, College of Pharmacy, The Ohio State University, 336 L. M. Parks Hall, Columbus, OH 43210. E-mail: chen.844{at}osu.edu

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