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Molecular Pharmacology, Vol 7, 260-268, Copyright © 1971 by the American Society for Pharmacology and Experimental Therapeutics

Evidence for a Medium-Chain Fatty Acid:Coenzyme A Ligase (Adenosine Monophosphate) That Activates Salicylate

PAUL G. KILLENBERG 1, EUGENE D. DAVIDSON 1, and LESLIE T. WEBSTER JR. 1

1 Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106

A medium-chain fatty acid:CoA ligase (AMP) which activates salicylate has been purified approximately 30-fold from bovine liver "mitochondria." The new enzyme is not completely free of a medium-chain fatty acid:CoA ligase (AMP) (EC 6.2.1.2) of the type first described by Mahler, Wakil, and Bock [J. Biol. Chem. 204, 453 (1953)], but the two enzymes can be distinguished readily from each other. The enzyme with salicyl-CoA synthetase activity is more easily inactivated, migrates more slowly toward the anode during disc gel electrophoresis, and utilizes a wider spectrum of aromatic acid substrates than the enzyme described earlier. Evidence was obtained that both enzymes could catalyze the activation of hexanoate, benzoate, o-methoxybenzoate, and anthranilate, but only the "salicylate" enzyme displayed activity with salicylate and p-aminosalicylate.

Note:
ACKNOWLEDGMENT The authors wish to thank Dr. Sung Ling Yuan for his valuable technical assistance.

Submitted on January 20, 1971






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