MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by POLAND, A.
Right arrow Articles by KAPPAS, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by POLAND, A.
Right arrow Articles by KAPPAS, A.

Molecular Pharmacology, Vol 7, 697-705, Copyright © 1971 by the American Society for Pharmacology and Experimental Therapeutics

The Metabolism of Aminopyrine in Chick Embryo Hepatic Cell Culture: Effects of Competitive Substrates and Carbon Monoxide

ALAN POLAND 1 and ATTALLAH KAPPAS 1

1 The Rockefeller University, New York, New York 10021

Chick embryonic liver cells grown in monolayer cultures metabolize aminopyrine to 4-aminoantipyrine, which is released into the medium. This N-demethylation is performed by liver cell cultures but not by renal or intestinal cell cultures from chick embryos. The formation of 4-aminoantipyrine is linear with time and is inhibited by SKF 525-A (2-diethylaminoethyl 2,2-diphenylvalerate HCl), piperonyl butoxide, hexobarbital, lauric acid, and testosterone. Inhibition in all cases is at least partially reversible. The rate of 4-aminoantipyrine formation is also reversibly inhibited by carbon monoxide. From the CO:O2 ratio and rate of formation of the metabolite, it is possible to estimate the partition coefficient, KL, for cytochrome P-450 in living cells, which agrees quite well with estimates from incubations in vitro.

Note:
ACKNOWLEDGMENT We would like to thank Mrs. Sandra Weinstein for excellent technical assistance.

Submitted on April 23, 1971






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1971 by the American Society for Pharmacology and Experimental Therapeutics