MolPharm xPharm- The Comprehensive Pharmacology Reference

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by STRIPP, B.
Right arrow Articles by GILLETTE, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by STRIPP, B.
Right arrow Articles by GILLETTE, J. R.

Molecular Pharmacology, Vol 8, 189-196, Copyright © 1972 by the American Society for Pharmacology and Experimental Therapeutics

An Approach Measurement of the Stoichiometric Relationship between Hepatic Microsomal Drug Metabolism and the Oxidation of Reduced Nicotinamide Adenine Dinucleotide Phosphate

B. STRIPP 1, N. ZAMPAGLIONE 1, M. HAMRICK 1, and J. R. GILLETTE 1

1 Laboratory of Chemical Pharmacology, National Heart and Lung Institute, National Institutes of Health Bethesda, Maryland 20014

Various methods for estimating the stoichiometric relationship between drug oxidation and NADPH oxidation in hepatic microsomes are discussed. Of these, two methods give values approaching 1:1 for a number of type I substrates. One method relates the metabolism to the substrate-dependent NADPH oxidation corrected for CO-sensitive, endogenous NADPH oxidation. The correction was obtained by measuring the rate of NADPH oxidation in an atmosphere of 9:1 CO-O2. The other method relates the CO-sensitive drug oxidation to CO-sensitive NADPH-oxidation.

Submitted on November 8, 1971






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1972 by the American Society for Pharmacology and Experimental Therapeutics