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Molecular Pharmacology, Vol 8, 565-574, Copyright © 1972 by the American Society for Pharmacology and Experimental Therapeutics

Investigation into the Association between Serotonin and Adenosine Triphosphate in Vitro by Nuclear Magnetic Resonance and Ultraviolet Spectroscopy

THOMAS NOGRADY 1, PAVEL D. HRDINA 1, and GEORGE M. LING 1

1 Department of Chemistry, Loyola College, Montreal 262, Quebec, and Department of Pharmacology, University of Ottawa, Ottawa 2, Ontario, Canada

Adenosine triphosphate and serotonin (5-hydroxytryptamine, 5-HT) form micellar complexes in synaptic storage vesicles and blood platelets. The interaction of ATP with 5-HT and the binding forces responsible for micelle formation were investigated by nuclear magnetic resonance and ultraviolet spectroscopy in vitro. The principal bond is an ionic interaction between the side chain amino group of 5-HT and the negatively charged phosphate of ATP. This results in the disruption of ATP stacking and overlap of the indole and adenine moiety, forming a contact charge-transfer complex with a low apparent association constant. pH and ionic strength effects on complex formation were also investigated.

Note:
ACKNOWLEDGMENTS The valuable help of Dr. M. Hogben of the Chemistry Department, Loyola College, in the computer simulation of 5-HT spectra and calculation of linewidths is gratefully acknowledged.

Submitted on March 28, 1972




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S. Nag, J. Balaji, P. K. Madhu, and S. Maiti
Intermolecular Association Provides Specific Optical and NMR Signatures for Serotonin at Intravesicular Concentrations
Biophys. J., May 15, 2008; 94(10): 4145 - 4153.
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