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Molecular Pharmacology, Vol 9, 421-425, Copyright © 1973 by the American Society for Pharmacology and Experimental Therapeutics

Incorporation of a Lysergic Acid Diethylamide Intermediate into Antibody Protein in Vitro

EDWARD W. VOSS JR. 1, PEYTON METZEL 1, and JEFFREY L. WINKELHAKE 1

1 Department of Microbiology, University of Illinois, Urbana, Illinois 61801

Antibody-producing lymphoid cells incubated in vitro in the presence of [3H]lysergic acid diethylamide secrete labeled protein. Incorporation of [3H]lysergic acid diethylamide (or a derivative) de novo was indicated by precipitation of labeled peptides with trichloracetic acid and by the retention of label after dialysis against denaturing agents and high concentrations of a dissociating ligand. Incorporation of [3H]lysergic acid diethylamide by immune lymphoid cells was inhibited by puromycin and enhanced by homologous antigen.

Note:
ACKNOWLEDGMENT We thank Dennis E. Lopatin for performing the equilibrium dialysis experiments in microdialysis chambers.

Submitted on November 29, 1972






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