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Molecular Pharmacology, Vol 9, 840-845, Copyright © 1973 by the American Society for Pharmacology and Experimental Therapeutics

Evidence Against Participation of Cytochrome b5 in the Hepatic Microsomal Mixed-Function Oxidase Reaction

INGELA JANSSON 1 and JOHN B. SCHENKMAN 1

1 Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510

The addition of increasing concentrations of a detergent (Triton X-100)-solubilized cytochrome b5 from liver microsomes to liver microsomes of untreated or phenobarbital-treated animals produced correspondingly greater inhibition of aminopyrine dealkylation. Even addition of NADH to the NADPH-supported reaction did not prevent this inhibition, although the added cytochrome b5 functioned as an endogenous hemoprotein; i.e., it was reduced by NADH and stimulated the reduction of exogenous cytochrome c. These observations suggest that cytochrome b5 does not function in hepatic microsomal mixedfunction oxidase reactions.

Submitted on July 2, 1973






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