QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text (PDF) Data Supplement All Versions of this Article: mol.105.018465v1 69/1/385    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, S. J. Articles by Kim, S. G. Search for Related Content PubMed PubMed Citation Articles by Lee, S. J. Articles by Kim, S. G.

Role of p90 Ribosomal S6-Kinase-1 in Oltipraz-Induced Specific Phosphorylation of CCAAT/Enhancer Binding Protein- for GSTA2 Gene Transactivation

National Research Laboratory, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea

Received August 29, 2005; accepted October 24, 2005

	Abstract

Top Abstract Materials and Methods Results Discussion References

 
Oltipraz, which has been extensively studied as a cancer chemopreventive agent, promotes phosphatidylinositol 3-kinase-mediated activation of CCAAT/enhancer binding protein- (C/EBP). Activated p90 ribosomal S6-kinase-1 (RSK1) phosphorylates major transcription factors, including C/EBP. This study examined whether oltipraz induces phosphorylation of C/EBP at specific residues, and if so, whether RSK1 regulates C/EBP phosphorylation by oltipraz for the GSTA2 gene transactivation. Subcellular fractionation and immunoblot analyses revealed that oltipraz treatment increased the level of C/EBP phosphorylated at Ser¹⁰⁵ in the cytoplasm, which translocated to the nucleus for DNA binding in rat H4IIE cells. Immunoprecipitation-immunoblot, chromatin-immunoprecipitation, and specific mutation analyses revealed that Ser¹⁰⁵-phosphorylated C/EBP recruited the cAMP response element-binding protein binding protein for histone acetylation and transactivation of the GSTA2 gene. The role of RSK1 in Ser¹⁰⁵-phosphorylation of C/EBP by oltipraz and its gene transactivation was evidenced by transfection experiments with dominant-negative mutants of RSK1. In mouse Hepa1c1c, human HepG2 cells, and rat primary hepatocytes, oltipraz induced phosphorylation of C/EBP at Thr²¹⁷, Thr²⁶⁶, and Ser¹⁰⁵, respectively, via RSK1. The experiment using small-interference RNA of RSK1 confirmed the essential role of RSK1 in the gene expression. Inhibition of PI3-kinase activity prevented oltipraz-inducible Ser¹⁰⁵-phosphorylation of rat C/EBP. Oltipraz treatment led to increases in the catalytic activity and nuclear translocation of RSK1, which was abrogated by PI3-kinase inhibition. In summary, oltipraz induces the phosphorylation of rat C/EBP at Ser¹⁰⁵ (functionally analogous Thr^217/266 in mouse and human forms) in hepatocytes, which results in cAMP response element-binding protein-binding protein (CBP) recruitment for the GSTA2 gene transactivation, and the specific C/EBP phosphorylation is mediated by RSK1 downstream of PI3-kinase.

The family of C/EBPs plays important roles in regulating the expression of hepatocyte-specific genes, particularly those associated with cell survival or proliferation (Diehl, 1998; Buck and Chojkier, 2003). We reported that oltipraz promotes nuclear translocation of CCAAT/enhancer binding protein- (C/EBP) and its DNA binding activity for transactivation of the GSTA2 gene, and that the pathway of phosphatidylinositol 3-kinase (PI3-kinase) regulates the activation of C/EBP (Cho and Kim, 2003b; Kang et al., 2003). In addition, we observed that mitogen-activated protein kinases (MAPKs), including ERK1/2, were neither activated by oltipraz nor involved in C/EBP-mediated gene expression (Kang et al., 2003). Yet, the kinase(s) responsible for the activation of C/EBP by oltipraz remained to be elucidated. Therefore, we proposed the hypothesis that oltipraz activates C/EBP by phosphorylation at specific site(s), which may be mediated by cellular kinase(s) downstream from PI3-kinase.

The members of p90 ribosomal S6-kinase (RSK) family play a critical role in mitogen-activated cell growth, differentiation, or survival (Bhatt and Ferrell, 1999; Frodin and Gammeltoft, 1999; Gross et al., 1999). Among the RSK isoforms, RSK1 is a major form expressed in the tissues, including liver, muscle, and fat (Moller et al., 1994). The RSK1 contains two distinct kinase domains that are functionally active, and the N-terminal kinase of activated RSK1 phosphorylates the cellular protein substrates, including C/EBP, cAMP response element-binding protein, c-Fos, and IB (Chen et al., 1993; Xing et al., 1996; Ghoda et al., 1997; Schouten et al., 1997; Buck et al., 1999; Frodin and Gammeltoft, 1999). Activation of RSK1 by growth factor requires extracellular signal-regulated kinase (ERK) docking near the C-terminal region (Roux et al., 2003), and the activated C-terminal kinase domain leads to autophosphorylation, located in the linker region (Vik and Ryder, 1997). Another phosphorylation by 3-phosphoinositide-dependent protein kinase-1 (PDK1) in the activation loop of the N-terminal kinase domain allows RSK1 to phosphorylate the target proteins (Jensen et al., 1999; Richards et al., 1999; Williams et al., 2000).

Receptor-activated signaling pathways regulate phosphorylation of C/EBP in its activation domain (Buck and Chojkier, 2003), which leads to the transcription of its target genes. It has been shown that RSK activated downstream from the transforming growth factor- receptor tyrosine kinase induces phosphorylation of C/EBP at specific residues such as Thr²¹⁷ in the mouse form (Buck et al., 1999). Phosphorylation of Thr²¹⁷ residue in mouse C/EBP (Thr²⁶⁶ in the human form) turned out to be essential for transactivation of target genes (Buck and Chojkier, 2003). Because rat C/EBP has evolved with a double mutation and thus lacks the phosphoacceptor, the C/EBP form has a compensatory Ser¹⁰⁵, whose phosphorylation is also catalyzed by RSK (Buck and Chojkier, 2003). Hence, Ser¹⁰⁵ residue in rat C/EBP and functionally analogous residues Thr²¹⁷ and Thr²⁶⁶ in mouse and human forms, respectively, are the critical phosphoacceptors that are responsible for gene transactivation (Buck et al., 1999). In addition, C/EBP phosphorylated at the specific residue by RSK1 nontranscriptionally prevents apoptosis of cells through the interaction with procaspases (Buck et al., 2001). Hence, RSK1-mediated specific phosphorylation of C/EBP regulates cell survival.

In view of the activation of C/EBP by oltipraz and the essential role of Ser¹⁰⁵ phosphorylation (analogous phosphorylations at Thr^217/266 in mouse and human) in gene transactivation, we investigated whether oltipraz induces C/EBP phosphorylation at the residue for the GSTA2 gene transactivation and, if so, whether the phosphorylation of C/EBP is mediated by RSK1. We additionally determined the effects of oltipraz on Thr²¹⁷ or Thr²⁶⁶ phosphorylation in the mouse and human forms of C/EBP, respectively, and the role of RSK1 in the phosphorylations. In addition, we verified the specific C/EBP phosphorylation by RSK1 in primary cultured rat hepatocytes. Toward the end, we explored what the role of PI3-kinase is in RSK1-mediated C/EBP phosphorylation by oltipraz. Now, we report that oltipraz induces specific C/EBP phosphorylation for the GSTA2 gene transactivation via RSK1 and that PI3-kinase contributes to the RSK1-mediated phosphorylation of C/EBP.

	Materials and Methods

Top Abstract Materials and Methods Results Discussion References

 
Materials. [-³²P]ATP (3000 mCi/mmol) was purchased from PerkinElmer Life and Analytical Sciences (Boston, MA). Anti-C/EBP, anti-C/EBP, anti-C/EBP, anti-Ser¹⁰⁵-phosphorylated C/EBP (sc-16994-R), anti-Thr²¹⁷-phosphorylated C/EBP (sc-16993-R), anti-CBP, anti-RSK1, anti-HA, anti-Myc, and anti-actin antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-acetylated histone antibody was purchased from Upstate Biotechnology (Waltham, MA). Anti-Thr¹⁸⁹-phosphorylated C/EBP, anti-ERK, and anti-Thr^42/44-phosphorylated ERK antibodies were supplied from Cell Signaling Technology (Beverly, MA). Horseradish peroxidase-conjugated goat anti-rabbit and rabbit anti-goat IgGs were purchased from Zymed Laboratories (San Francisco, CA). U0126 was obtained from Alexis Corporation (Läufelfingen, Switzerland). LY294002 and other reagents in the molecular studies were supplied from Calbiochem (Darmstadt, Germany). S6 rsk substrate peptide was purchased from Santa Cruz Biotechnology. The plasmid of C/EBP-containing GSTA2 promoter region (-1651 bp to +66 bp) was kindly provided by Dr. C. B. Pickett (Schering Plough, Kenilworth, NJ). The plasmids encoding HA-C-terminal truncated (CTT)-RSK1 and HA-K112/464R-RSK1 were kind gifts from Dr. J. Blenis (Harvard Medical School, Boston, MA). The overexpression vector of p85 subunit of PI3-kinase was obtained from Dr. A. Toker (The Boston Biomedical Research Institute, Boston, MA). MKK1 dominant-negative mutant was a gift from Dr. N G. Ahn (University of Colorado, Boulder, CO).

Cell Culture. Rat H4IIE, mouse Hepa1c1c, and human HepG2 cells were obtained from American Type Culture Collection (Manassas, VA). Primary hepatocytes were isolated from male Sprague-Dawley rats according to the method published previously, with slight modifications (Buck et al., 2001; Kang et al., 2003). Cells were maintained in Dulbecco's modified Eagle's medium containing 10% fetal calf serum (FCS), 50 U/ml penicillin, and 50 µg/ml streptomycin at 37°C in a humidified atmosphere with 5% CO₂. After deprivation of serum for 24 h, the cells were incubated with oltipraz (CJ Corp., Seoul, Korea) and dissolved in dimethyl sulfoxide for the indicated time period at 37°C.

Subcellular Fractionation. Total cell lysates, cytosolic fractions, and nuclear extracts were prepared according to methods published previously (Park et al., 2004). In brief, cells were centrifuged at 2300g for 3 min and allowed to swell after the addition of the lysis buffer. The lysate samples were centrifuged at 10,000g for 10 min to obtain cell lysates. To prepare cytosolic fractions and nuclear extracts, cells were centrifuged at 2300g for 3 min and allowed to swell after the addition of 100 µl of hypotonic buffer. The lysates were incubated for 10 min on ice and then centrifuged at 7200g for 5 min at 4°C. The supernatants were used as cytosolic fractions. Pellets containing crude nuclei were resuspended in 50 µl of extraction buffer. Nuclear extracts were prepared from the samples by centrifugation at 15,000g for 10 min and stored -70°C until use. Protein content was determined by the Bradford assay (Bio-Rad protein assay kit; Bio-Rad, Hercules, CA).

Immunoblot Analysis. SDS-polyacrylamide gel electrophoresis and immunoblot analysis were performed according to procedures published previously (Kang et al., 2003).

Gel Shift Assay. A double-stranded probe containing the C/EBP consensus oligonucleotide was used for gel shift analysis after end-labeling of the probe with [-³²P]ATP and T₄ polynucleotide kinase, as described previously (Kang et al., 2003; Park et al., 2004). Specificity of binding was determined by competition experiments, known as immunoinhibition assays. For immunoinhibition assays, anti-Ser¹⁰⁵-phosphorylated C/EBP, anti-Thr¹⁸⁹-phosphorylated C/EBP, or anti-Sp1 antibody (1 µg each) was added to the reaction mixture after initial 10-min incubation and additionally incubated with the probe for 30 min at 25°C. Samples were loaded onto 4% polyacrylamide gels at 100 V. The gels were removed, fixed, and dried, followed by autoradiography.

Immunoprecipitation. To determine the physical interaction of CBP with Ser¹⁰⁵-phosphorylated C/EBP, a fraction of cell lysates (100-µg proteins in 300 µl) was incubated with a polyclonal rabbit anti-CBP antibody overnight at 4°C. The antigen-antibody complex was immunoprecipitated after incubation for 2 h at 4°C with protein G-agarose. Immune complex was solubilized in 2x Laemmli buffer and boiled for 5 min. Samples were separated and analyzed using 7.5% SDS-polyacrylamide gel electrophoresis and then transferred to nitrocellulose membranes. The samples were then immunoblotted with antibodies directed against Ser¹⁰⁵- or Thr¹⁸⁹-phosphorylated C/EBP. Blots were developed using an ECL chemiluminescence detection kit.

Chromatin Immunoprecipitation Assays. H4IIE cells were treated with oltipraz for 12 h, and then formaldehyde was added to the cells to a final concentration of 1%. Ser¹⁰⁵-phosphorylated C/EBP, CBP, or acetylated histone was cross-linked to chromatin by incubating the cells for 10 min at 37°C. The cells were washed with ice-cold phosphate-buffered saline and lysed in the Tris-HCl buffer (50 mM), pH 8.1, containing 1% SDS and 10 mM EDTA. The lysates were sonicated and centrifuged at 10,000g for 10 min to remove debris. The supernatants containing chromatin were diluted with 10 volumes of the chromatin immunoprecipitation (ChIP) dilution buffer (16.7 mM Tris-HCl, pH 8.1, 167 mM NaCl, 1.2 mM EDTA, 0.01% SDS, and 1.1% Triton X-100). One tenth of the chromatin solution was reserved for total input. The remaining solution was precleared with protein G-agarose, subsequently incubated with each antibody (1 µg) for 12 h at 4°C with shaking, and then further incubated with protein G-agarose for 2 h. The immunoprecipitates were washed, reverse cross-linked by adding 5 M NaCl to a final concentration of 200 mM, and incubated for 4 h at 65°C, as described previously (Duong et al., 2002). ChIP assay was also carried out with anti-Thr¹⁸⁹-phosphorylated C/EBP antibody, which was used as a negative control. DNA was phenol-chloroform-extracted. PCR was performed with specific primers flanking the C/EBP binding site in the GSTA2 gene promoter (sense: 5'-GGACAACACACTCAGCTTTG-3'; antisense: 5'-TCAGTGCAGCCTGTGAGTC-3') or flanking the -actin gene promoter (sense: 5'-CGTTCCGAAATTGCCTTTTA-3'; antisense: 5'-GGAGCTGCAAGGAGGTTGTA-3'). Amplified fragments (347 bp, 121 bp) were analyzed on a 2% agarose gel.

Mutagenesis Assay. C/EBP amplified from pCDNA3.1(+)-rat C/EBP (Cho and Kim, 2003a) using specific primers was inserted into pGEM-T vector (Promega, Madison, WI) and subcloned into the BamHI/HindIII sites of the pCMV-Tag3A plasmid (Stratagene, La Jolla, CA). Specific base substitution was made by oligonucleotide-mediated mutagenesis according to the manufacturer's instruction (Stratagene). Ser¹⁰⁵ residue in rat C/EBP was mutated to alanine using a mutagenic primer (5'-GTAACCGTAGTCGGCCGGCTTCTTGCTCGG-3'). The DNA sequence was verified by using an automatic DNA sequence analyzer.

Transient Transfection and pGL-1651 Promoter-Luciferase Assay. To determine the activity of C/EBP-mediated target gene transactivation, we used the pGL1651-luciferase reporter assay system according to the procedures published previously (Cho and Kim, 2003b; Park et al., 2004). Cells were transiently transfected with pGL1651-promoter luciferase construct in combination with the plasmid of pCMV-Tag3A-C/EBP or pCMV-Tag3A-C/EBP-S105A (Ala¹⁰⁵ mutant of rat C/EBP). In some experiments, HA-CTT-RSK1 or HA-K112/464R-RSK1 plasmid was cotransfected with the pGL-1651 construct. In brief, cells (5 x 10⁵ cells/well) were replated in six-well plates overnight, serum-starved for 6 h, and transiently transfected with 1 µg of pGL-1651-luciferase construct and 0.3 µg of pCMV--galactosidase plasmid in the presence of Lipofectamine reagent (both from Invitrogen, Carlsbad, CA) for 3 h. The pCMV--galactosidase plasmid was used to evaluate the transfection efficiency. Transfected cells were incubated in Dulbecco's modified Eagle's medium containing 1% FCS for 3 h and exposed to oltipraz for 18 h at 37°C. For -galactosidase activity, 10 µg of cell lysates was added to the solution containing 0.88 mg/ml o-nitrophenyl--D-galactopyranoside, 100 µM MgCl₂, and 47 mM -mercaptoethanol in 100 mM sodium phosphate buffer. The reaction mixture was incubated for 12 h at 37°C, and the absorbance was determined at 420 nm. The relative luciferase activity was calculated by normalizing firefly luciferase activity to that of -galactosidase.

H4IIE, Hepa1c1c, HepG2 cells, or primary cultured rat hepatocytes were also transiently transfected with the plasmid encoding HA-CTT-RSK1 or HA-K112/464R-RSK1 and incubated for the indicated time period to assess the extent of C/EBP phosphorylation.

Knockdown Experiment Using siRNA. To knockdown RSK1, HepG2 cells were transfected with the siRNA against human RSK1 (Silencer-validated siRNA against RSK1, Ambion, Austin, TX). pGL-1651-promoter luciferase construct was cotransfected with the RSK siRNA or a nonspecific scRNA (100 pmol/ml) using Lipofectamine 2000 according to the manufacturer's instructions. On day 3 after transfection, the cells were incubated with oltipraz for 18 h. The whole lysates were used for the luciferase activity assay. Immunoblot analysis confirmed RSK1 knockdown 3 days after transfection.

Stable Transfection. For the preparation of PI3-kinase p85 [p85(+)] or MKK1 dominant-negative mutant [MKK1(-)] cells, H4IIE cells were transfected with the respective plasmid and incubated for 48 h, as described previously (Cho and Kim, 2003b; Kang et al., 2003). Geneticin was added to select the resistant colonies.

RSK1 Kinase Assay. Cells that had been incubated in the medium without serum for 24 h were treated with vehicle or oltipraz (30 µM) for the indicated time period, harvested, and lysed in the buffer containing 25 mM Tris-HCl, pH 7.4, 2 mM dithiothreitol, 10 mM MgCl₂, 5 mM -glycerophosphate, 1 mM Na₃VO₄, and 1 mM phenylmethylsulfonyl fluoride. RSK1 in cell lysates (300 µg) was immunoprecipitated with anti-RSK1 antibody. Immunoprecipitates were washed three times in lysis buffer, and once in kinase buffer containing Tris-HCl (25 mM, pH 7.4), 10 mM MgCl₂, 25 mM -glycerophosphate, 1 mM Na₃VO₄, 2 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, 1 µg/ml leupeptin, and 200 µM ATP. Kinase reaction was initiated by adding S6 rsk substrate peptide (5 µg per assay) and 2 µCi of [-³²P]ATP to a 20-µl reaction mixture, and continued for 30 min at 30°C. After brief centrifugation, the supernatant of reaction mixture was spotted onto p81 phosphocellulose paper (Upstate Biotechnology). The paper was washed with 0.8% phosphoric acid for 5 min three times and subsequently with 90% ethanol for 5 min. The membrane was dried and transferred to 5 ml of scintillation cocktail, and the radioactivity of phosphorylated substrate was measured using a -counter (PerkinElmer Wallac, Gaithersburg, MD).

Statistical Analysis. Scanning densitometry of the immunoblots was performed with Image Scan and Analysis System (Alpha Innotech, San Leandro, CA). The area of each lane was integrated using the software AlphaEase, version 5.5, followed by background subtraction. One-way analysis of variance was used to assess statistical significance of differences among treatment groups. For each statistically significant effect of treatment, the Newman-Keuls test was used for comparisons between multiple group means. The data were expressed as means ± S.E. The criterion for statistical significance was set at p < 0.05 or < 0.01.

	Results

Top Abstract Materials and Methods Results Discussion References

 
Induction of C/EBP by Oltipraz. Our previous study showed that oltipraz induces nuclear translocation of C/EBP and promotes C/EBP binding to the C/EBP binding site (Kang et al., 2003). In view of the importance of C/EBPs as transcriptional factors, we sought to determine the expression of major forms of C/EBP in H4IIE cells treated with oltipraz. Immunoblot analysis revealed that the levels of C/EBP were increased 12 to 48 h after oltipraz treatment compared with untreated control (Fig. 1), whereas those of C/EBP and forms were unaffected. Thus, activation of C/EBP by oltipraz involves the induction of C/EBP.

View larger version (35K): [in this window] [in a new window]   Fig. 1. Expression of C/EBP isoforms in cells treated with oltipraz. The levels of C/EBP, C/EBP, and C/EBP isoforms were determined in the lysates of cells treated with oltipraz for 3 to 48 h. Each lane was loaded with 20 µg of cell lysates. Actin was used as a control. Results were confirmed by repeated experiments.

View larger version (24K): [in this window] [in a new window]   Fig. 2. Alignment of RSK1- or ERK-phosphorylated residues in rat, mouse, and human homologous forms of C/EBP. A, alignment of the partial sequences of rat, mouse, and human C/EBP. The residues that are equivalent to Ser105, Thr189, and Ala218 in rat C/EBP are highlighted for comparison. B, the specific residues that are phosphorylated by RSK1 or ERK. The residues in rat, mouse, and human homologous forms of C/EBP phosphorylated by RSK1 or ERK were indicated.

Ser¹⁰⁵ Phosphorylation of C/EBP by Oltipraz.

View larger version (33K): [in this window] [in a new window]   Fig. 3. Oltipraz activation of C/EBP by Ser105 phosphorylation. A, immunoblot analysis of phosphorylated C/EBP. The levels of C/EBP phosphorylated at Ser105 or Thr189 were determined by immunoblot analyses in lysates prepared from H4IIE cells treated with oltipraz for 3 to 48 h (left). The levels of phosphorylated C/EBP were also determined in the cytosolic and nuclear fractions of oltipraz-treated cells (3-24 h) (middle and right). Equal loading of proteins was verified by probing the replicate blots for actin. Each lane contained 20 µg of lysate (or cytosolic) proteins or 10 µg of nuclear proteins. B, gel shift analysis of Ser105-phosphorylated C/EBP binding to the C/EBP binding site. Nuclear extracts were prepared from H4IIE cells cultured with 30 µM oltipraz for 12 h. All lanes contained 10 µg of nuclear extracts and 5 ng of labeled C/EBP consensus oligonucleotide. Immunoinhibition assays were carried out by incubating the nuclear extracts (oltipraz, 12 h) with the polyclonal antibody directed against Ser105- or Thr189-phosphorylated C/EBP or Sp1. Arrowheads indicate shifted DNA bound with Ser105-phosphorylated C/EBP. Results were confirmed by repeated experiments.

Next, we confirmed the formation of C/EBP-DNA binding complexes after oltipraz treatment (Fig. 3B). To determine whether the increase in the band intensity by oltipraz obtained in gel shift assays occurred as a result of phosphorylation of C/EBP at Ser¹⁰⁵, an immunoinhibition experiment was performed with the antibody directed against Ser¹⁰⁵- or Thr¹⁸⁹-phosphorylated C/EBP. The presence of anti-Ser¹⁰⁵-phosphorylated C/EBP antibody completely abolished the band intensity of the C/EBP-DNA binding complex, whereas either anti-Thr¹⁸⁹-phosphorylated C/EBP or anti-Sp1 antibody failed to do so (Fig. 3B). The antibody competition assays verified that oltipraz-induced C/EBP-DNA binding activity is specifically dependent on Ser¹⁰⁵-phosphorylated C/EBP. These data provided evidence that oltipraz treatment led to an increase in C/EBP phosphorylation at Ser¹⁰⁵, but not Thr¹⁸⁹, in H4IIE cells and that Ser¹⁰⁵-phosphorylated C/EBP served as an active form in the formation of C/EBP-DNA binding complex.

Association of Ser¹⁰⁵-Phosphorylated C/EBP with CBP. To investigate whether an increase in Ser¹⁰⁵ phosphorylation of C/EBP enhanced recruitment of CBP coactivator for gene transactivation, H4IIE cells were serum-starved for 24 h and then treated with oltipraz for the indicated time periods. Nuclear extracts prepared from untreated cells or cells treated with oltipraz were immunoprecipitated with anti-CBP antibody and then immunoblotted with anti-Ser¹⁰⁵-phosphorylated C/EBP antibody. Formation of CBP-Ser¹⁰⁵-phosphorylated C/EBP complex was increased after stimulation of cells with oltipraz for 12 to 24 h (Fig. 4A), during which time period the level of Ser¹⁰⁵-phosphorylated C/EBP in the nuclear fraction maximally increased (Fig. 4A, right). In contrast, Thr¹⁸⁹-phosphorylated C/EBP, immunoprecipitated with CBP, was unchanged. These data provide evidence that oltipraz treatment enhances the level of Ser¹⁰⁵-phosphorylated C/EBP that is capable of interacting with CBP coactivator.

View larger version (26K): [in this window] [in a new window]   Fig. 4. Recruitment of CBP to Ser105-phosphorylated C/EBP. A, association of CBP with Ser105-phosphorylated C/EBP. Interaction of CBP with Ser105- or Thr189-phosphorylated C/EBP was determined in H4IIE cells treated with 30 µM oltipraz for 3 to 24 h. Whole-cell lysates were precipitated with anti-CBP antibody and immunocomplexes were immunoblotted with anti-Ser105-phosphorylated or anti-Thr189-phosphorylated C/EBP antibody. Aliquots from the input were loaded for immunoblot of actin. Results were confirmed by repeated experiments. B, ChIP assays. The DNA-protein complexes prepared from cells treated with vehicle or oltipraz (30 µM, 12 h) were immunoprecipitated with anti-Ser105-phosphorylated C/EBP, anti-CBP, anti-acetylated histone, or anti-Thr189-phosphorylated C/EBP antibody. The samples were PCR-amplified using primers flanking the proximal and distal regions of the DNA comprising the C/EBP binding site in the GSTA2 promoter. A set of control experiment was carried out for the -actin gene. One tenth of the total input was used as a loading control. Results were confirmed by repeated experiments. C, specific mutagenesis assay. The effect of specific mutation of Ser105 residue in C/EBP on oltipraz-inducible luciferase expression from pGL-1651 GSTA2 promoter was assessed in H4IIE cells. Luciferase activities were measured in cells treated with vehicle or oltipraz (30 µM, 18 h) or in cells transfected with the plasmid encoding Myc-C/EBP. Data represented the mean ± S.E. with four separate experiments (significant compared with vehicle; *, p < 0.05). In another set of experiment, oltipraz-inducible change in luciferase expression was determined in cells transfected with the plasmid encoding Myc-C/EBP or Myc-C/EBP-S105A. Values were expressed as the changes relative to the respective vehicle-treated control and represented the mean ± S.E. with four to five separate experiments (significant compared with Myc-C/EBP; #, p < 0.05). Immunoblot analysis confirmed the expression of Myc-C/EBP or Myc-C/EBP-S105A.

Next, to determine the association of Ser¹⁰⁵-phosphorylated C/EBP with CBP on the target gene promoter, we performed ChIP analysis. The DNA-protein complexes were immunoprecipitated with anti-Ser¹⁰⁵-phosphorylated C/EBP, anti-CBP, or anti-acetylated histone antibody, followed by reversal of cross-linking and PCR amplification using primers flanking the proximal and distal regions of the DNA comprising the C/EBP binding site in the GSTA2 gene promoter (Fig. 4B). In the cells treated with oltipraz (12 h), the intensities of the three PCR products were all distinctly higher compared with vehicle-treated control. The intensities of the PCR products using primers flanking the proximal and distal regions of the -actin gene promoter (a housekeeping gene) were unaffected by oltipraz. In the sample immunoprecipitated with anti-acetylated histone antibody, the intensity of the PCR product from the -actin gene was intense in control cells but was not further increased after oltipraz treatment (Fig. 4B). In contrast to the result obtained with Ser¹⁰⁵-phosphorylated C/EBP immunoprecipitate, the band intensity in Thr¹⁸⁹-phosphorylated C/EBP immunoprecipitate was not enhanced by oltipraz.

Next, we assessed the functional role of Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz in target gene transactivation by the specific mutagenesis assay. Oltipraz treatment was capable of increasing luciferase expression from the pGL-1651 GSTA2 promoter that contains the C/EBP binding site (Fig. 4C, left). Likewise, the expression of Myc-C/EBP promoted the gene transcription. Exposure of cells transfected with the myc-C/EBP plasmid to oltipraz for 18 h resulted in a greater increase in luciferase expression (i.e., 1.6-fold increase relative to C/EBP alone) (Fig. 4C, right). The expression of Myc-Ala¹⁰⁵ mutant of C/EBP, compared with that of Myc-C/EBP, completely abolished the ability of oltipraz to promote luciferase expression from pGL-1651 (Fig. 4C, right). These results indicate that Ser¹⁰⁵ phosphorylation of C/EBP, which is promoted by oltipraz treatment, leads to recruitment of CBP to the GSTA2 gene promoter and enhances histone acetylation for the gene transcription.

Ser¹⁰⁵ Phosphorylation of C/EBP by RSK1. In view of the role of RSK1 in the phosphorylation of C/EBP, we sought to determine whether RSK1 is responsible for Ser¹⁰⁵ phosphorylation of C/EBP. Transfection with the KH3 plasmid, a control vector, allowed cells to phosphorylate C/EBP at Ser¹⁰⁵ in response to oltipraz (30 µM, 12 h). In contrast, expression of HA-CTT-RSK1 or constitutively inactive kinase-dead mutant of RSK1 (HA-K112/464R-RSK1) completely inhibited oltipraz enhancement in Ser¹⁰⁵ phosphorylation of C/EBP, as determined by immunoblot analyses in lysates (Fig. 5A). In contrast, Thr¹⁸⁹ phosphorylation was unchanged by the plasmids. In parallel with this, the increase in the level of nuclear Ser¹⁰⁵-phosphorylated C/EBP was prevented by overexpression of HA-CTT-RSK1 or HA-K112/464R-RSK1 (Fig. 5A).

View larger version (25K): [in this window] [in a new window]   Fig. 5. The role of RSK1 in Ser105 phosphorylation of C/EBP and gene transactivation by oltipraz. A, the levels of Ser105-phosphorylated C/EBP. The levels of C/EBP phosphorylated at Ser105 or Thr189 were determined by immunoblot analyses in lysates or nuclear fractions prepared from H4IIE cells that had been treated with oltipraz (12 h) after transfection with the HA-tagged plasmid encoding a truncated RSK1 (CTT-RSK1) or a kinase-dead mutant form of RSK1 (K112/464R-RSK1). Each lane was loaded with 20 µg of lysates or 10 µg of nuclear proteins. Expression of HA-CTT-RSK1 or HA-K112/464R-RSK1 was verified by immunoblotting for HA. Equal loading of proteins in each lane was verified by probing the replicate blot for actin. B, repression of C/EBP-mediated promoter luciferase activity by dominant-negative mutants of RSK1. Cells were cotransfected with the pGL-1651 and CMV--galactosidase plasmids (33:1) in combination with KH3 (empty vector), HA-CTT-RSK1 or HA-K112/464R-RSK1 plasmid at a ratio of 1:1 and the cells were exposed to oltipraz for 18 h. Activation of the reporter gene was calculated as a relative change to -galactosidase activity. The value for luciferase activity was expressed as relative luciferase unit of cell lysates and represented the mean ± S.E. with four separate experiments (significant compared with KH3 in untreated cells; *, p < 0.05, KH3 in untreated cells = 100%).

Thereafter, we determined the effects of dominant-negative mutants of RSK1 on oltipraz-inducible expression of the pGL-1651 luciferase reporter gene (Kang et al., 2003). As expected, transfection of cells with HA-CTT-RSK1 or HA-K112/464R-RSK1 entirely inhibited the ability of oltipraz to stimulate reporter gene expression from pGL-1651 (Fig. 5B). KH3, which was used as a control, did not inhibit the reporter gene expression. This finding indicates that RSK1 mediates Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz for GSTA2 gene transactivation.

RSK1-Dependent Phosphorylation of C/EBP in Other Species. We then determined whether oltipraz induced phosphorylation of the residue in mouse or human C/EBP functionally analogous to Ser¹⁰⁵ of rat C/EBP. Immunoblot analysis revealed that phosphorylation of C/EBP at Thr²¹⁷ or Thr²⁶⁶ was promoted by oltipraz (30 µM, 12 h) in mouse Hepa1c1c cells and human HepG2 cells, respectively (Fig. 6A). Increases in C/EBP phosphorylation at the Thr^217/266 residues by oltipraz were abolished by transfection with the plasmid encoding HA-K112/464R-RSK1. Hence, RSK1 contributes to Thr^217/266 phosphorylation by oltipraz in mouse and human C/EBP. In addition, we verified the role of RSK1 in Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz in primary cultured rat hepatocytes. Oltipraz (30 µM, 12 h) was capable of inducing C/EBP phosphorylation at Ser¹⁰⁵ in the primary hepatocytes (Fig. 6B). As expected, a dominant-negative mutant of RSK1 prevented oltipraz-inducible phosphorylation of C/EBP.

View larger version (25K): [in this window] [in a new window]   Fig. 6. The functional role of RSK1 in C/EBP phosphorylation in mouse, human cell lines, or primary hepatocytes. A, RSK1-mediated Thr217/266 phosphorylation by oltipraz in mouse and human C/EBP. The levels of Thr217- or Thr266-phosphorylated C/EBP were assessed by immunoblot analyses in lysates prepared from mouse Hepa1c1c or human HepG2 cells that had been treated with oltipraz (30 µM, 12 h) after transfection with the plasmid encoding HA-K112/464R-RSK1. B, the role of RSK1 in Ser105 phosphorylation of C/EBP by oltipraz in primary cultured rat hepatocytes. Rat hepatocytes were incubated in the medium containing 10% FCS for 24 h, transiently transfected with the plasmid encoding HA-K112/464R-RSK1 using Lipofectamine 2000, and then treated with oltipraz for 12 h, as described under Materials and Methods. Control cells were transfected with KH3 and empty plasmid. Equal loading of proteins in each lane was verified by probing the replicate blot for actin. Expression of HA-K112/464R-RSK1 was verified by immunoblotting for HA. Results were confirmed by separate experiments. C, the effect of RSK1 knockdown on the C/EBP-mediated gene transactivation by oltipraz. HepG2 cells were transfected with RSK1 siRNA or scRNA in combination with pGL-1651, incubated for 3 days, and then treated with vehicle or 10 µM oltipraz for 18 h. The luciferase expression from pGL-1651 was analyzed in cell lysates. Aliquots of the samples were subjected to immunoblot analyses. Values were expressed as the changes relative to the respective vehicle-treated control and represented the mean ± S.E. with three separate experiments (significant compared with scRNA transfection: **, p < 0.01).

In addition, we used the knockdown technique to verify the functional role of RSK1 in the GSTA2 gene transactivation by oltipraz. Knockdown of RSK1 by transfection of HepG2 cells with the siRNA that specifically catalyzes degradation of human RSK1 mRNA resulted in a substantial decrease in the luciferase expression from pGL-1651 (Fig. 6C, left). In this experiment, scrambled RNA (scRNA) was used as a nonspecific RNA. Immunoblot analysis confirmed a decrease in RSK1 expression by the siRNA (Fig. 6C, right). The human RSK1 siRNA failed to degrade rat RSK1 (Supplemental Data S1), which confirmed its specificity.

Effects of MKK1 Inhibition on Oltipraz Activation of C/EBP and Gene Expression. Activation of ERK initiates an activating process of RSK1 by epidermal growth factor (Roux et al., 2003). To study whether the MKK1/ERK pathway was involved in the increase in Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz, we assessed the effect of U0126, an MKK1 inhibitor, on the phosphorylation in lysates. U0126 weakly but insignificantly prevented an increase in Ser¹⁰⁵ phosphorylation of C/EBP in the lysates prepared from H4IIE cells treated with oltipraz for 12 h (Fig. 7A, left). We also monitored the levels of Ser¹⁰⁵-phosphorylated C/EBP in H4IIE cells or cells stably transfected with dominant-negative mutant of MKK1 [MKK1(-)] (Fig. 7A, right). Increase in nuclear Ser¹⁰⁵-phosphorylated C/EBP by oltipraz was marginally repressed by MKK1(-) transfection. U0126 treatment or MKK1(-) transfection completely inhibited the activation of ERK1/2 by insulin-like growth factor (100 ng/ml, 10 min). Therefore, it is unlikely that an increase in Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz is under the control of ERK1/2.

View larger version (33K): [in this window] [in a new window]   Fig. 7. The role of ERK in C/EBP activation by oltipraz. A, the effects of MKK1 inhibition on Ser105 phosphorylation of C/EBP in cell lysates. The effect of ERK in Ser105 phosphorylation of C/EBP was assessed by using U0126 or MKK1(-) stable transfection. H4IIE cells that had been treated with U0126 (10 µM, 1 h) were further incubated with oltipraz (30 µM, 12 h) in the continuing presence of U0126. Cells were stably transfected with the plasmid encoding MKK1(-). Inhibition of ERK activation by U0126 treatment or MKK1(-) transfection was confirmed by immunoblotting phosphorylated ERK1/2 and total ERK1/2 (p-ERK and ERK, respectively) in cells exposed to insulin-like growth factor (IGF, 100 ng/ml, 10 min). B, the role of ERK in nuclear translocation of Ser105-phosphorylated C/EBP. Immunoblot analyses were performed with the nuclear fractions prepared from cells treated as described in A. Data represent the mean ± S.E. with four separate experiments (significant compared with control: *, p < 0.05; **, p < 0.01) (N.S., not significant). C, the effect of MKK1(-) transfection on oltipraz-inducible luciferase-reporter activity. Luciferase activity was measured in lysates of H4IIE cells treated with vehicle or oltipraz (18 h) after transfection with PCMV (empty vector) or MKK1(-). Cells were transfected with pGL-1651-luciferase reporter plasmid, as described in Fig. 3C. Data represented the mean ± S.E. with four separate experiments (significant compared with control: *, p < 0.05).

PI3-Kinase-Dependent Ser¹⁰⁵ Phosphorylation. The pathway of PI3-kinase is involved in a number of cellular responses by growth stimuli (Katso et al., 2001). We reported previously that C/EBP activation is dependent on the activity of PI3-kinase (Kang et al., 2003). To determine whether Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz was controlled by PI3-kinase, we measured the levels of Ser¹⁰⁵-phosphorylated C/EBP in lysates or nuclear fractions. H4IIE cells were incubated with LY294002 (10 µM), a chemical inhibitor of PI3-kinase, for 1 h and then exposed to oltipraz (30 µM). Immunoblot analysis revealed that LY294002 blocked oltipraz-inducible phosphorylation of C/EBP at Ser¹⁰⁵ in lysates (Fig. 8A). In addition, increase in the level of nuclear Ser¹⁰⁵-phosphorylated C/EBP was almost completely abolished by treatment with LY294002 or stable transfection with the p85(+) subunit of PI3-kinase (Fig. 8B). Additional immunoblot assays showed that the levels of total C/EBP were unchanged by LY294002 treatment (data not shown), indicating that prevention of oltipraz-inducible Ser¹⁰⁵ phosphorylation of C/EBP by PI3-kinase inhibition was not caused by a decrease in C/EBP expression. Our results showed that PI3-kinase regulated Ser¹⁰⁵ phosphorylation of C/EBP.

View larger version (20K): [in this window] [in a new window]   Fig. 8. PI3-kinase-dependent Ser105-phosphorylation of C/EBP by oltipraz. A, the levels of Ser105-phosphorylated C/EBP in cell lysates. H4IIE cells that had been serum-starved for 24 h were pretreated with LY294002 (10 µM) for 1 h and further incubated with 30 µM oltipraz for 12 h in the continuing presence of LY294002. Ser105-phosphorylated C/EBP was assessed by immunoblot analysis in cell lysates. Equal loading of proteins was verified by probing the replicate blots for actin. B, the levels of nuclear Ser105-phosphorylated C/EBP. Nuclear proteins obtained from cells treated with oltipraz (30 µM, 12 h) in the presence or absence of 10 µM LY294002 were subjected to immunoblot analysis. Cells stably expressing the p85 subunit of PI3-kinase were used to assess the role of PI3-kinase in Ser105 phosphorylation of C/EBP by oltipraz. The relative levels of Ser105-phosphorylated C/EBP were assessed by scanning densitometry of the immunoblots. Data represent the mean ± S.E. with three separate experiments (significant compared with control: **, p < 0.01; significant compared with oltipraz alone: ##, p < 0.01; control level = 1); p85(+), overexpression of p85 subunit.

View larger version (13K): [in this window] [in a new window]   Fig. 9. PI3-kinase-dependent RSK1 activation by oltipraz. A, increase in the kinase activity of RSK1 by oltipraz. H4IIE cells were incubated with 30 µM oltipraz for the indicated time periods. RSK1 activity toward S6 rsk substrate peptide was determined in cell lyates by monitoring 32P radioactivity. Data represent the mean ± S.E. with three separate experiments (significant compared with control: *, p < 0.05; **, p < 0.01; zero time control = 1). B, the effect of PI3-kinase inhibition on the kinase activity of RSK1. H4IIE cells were treated with vehicle or oltipraz in the presence or absence of LY294002 (10 µM) for 1 h. The kinase activity was measured as described above. Values are expressed as the change in RSK1 activity relative to vehicle-treated control and represented the mean ± S.E. with four separate experiments (significant compared with control: *, p < 0.05; significant compared with oltipraz treatment: #, p < 0.05). C, the effect of PI3-kinase inhibition on the nuclear translocation of RSK1 induced by oltipraz. Immunoblot analysis was performed in the nuclear fractions prepared from cells treated with oltipraz in the presence or absence of LY294002 (10 µM) for 12 h. Equal loading of proteins was verified by probing the replicate blot for actin. Each lane contained 10 µg of proteins. Results were confirmed by three separate experiments and a representative blot is shown.

	Discussion

Top Abstract Materials and Methods Results Discussion References

The immunoprecipitation and ChIP assays demonstrated that Ser¹⁰⁵-phosphorylated C/EBP was functionally active in gene transcription. N-terminal transactivation domain of C/EBP may interact with CBP/p300 coactivator, which is critical for C/EBP-mediated gene transactivation (Mink et al., 1997). In the present study, we revealed that oltipraz treatment increased the level of Ser¹⁰⁵-phosphorylated C/EBP that is capable of binding to CBP, inducing histone acetylation for the GSTA2 gene transactivation. The C/EBP gene contains the C/EBP binding site(s) in the promoter region (GenBank accession number 178567) (Mink et al., 1999). The role of Ser¹⁰⁵-phosphorylated C/EBP in gene transactivation was additionally supported by the finding that oltipraz specifically increased the expression of C/EBP but not C/EBP or C/EBP. Thus, it is highly likely that oltipraz induction of C/EBP after initial activation of pre-existing C/EBP by Ser¹⁰⁵ phosphorylation contributes to persistent gene transactivation. Although Thr¹⁸⁹ phosphorylation of rat C/EBP was unchanged after oltipraz treatment, we observed that Thr¹⁸⁹-phosphorylated C/EBP constitutively interacted with CBP and bound to the promoter region of the GSTA2 gene (Fig. 3). Hence, the Thr¹⁸⁹ phosphorylation might be responsible for the constitutive gene expression. Activation of C/EBP may be mediated by multiple phosphorylations at the serine or threonine residues within the molecule (Buck and Chojkier, 2003). In the present study, the specific mutagenesis analysis of C/EBP lends support to the essential role of Ser¹⁰⁵ phosphorylation for oltipraz's inducible gene transcription, as evidenced by the complete abrogation of oltipraz's increase in C/EBP-mediated gene expression in cells transfected with Myc-C/EBP-S105A. Our results provide compelling evidence that Ser¹⁰⁵ plays a critical role in C/EBP activation by oltipraz.

Rat C/EBP is known to be phosphorylated at the residue of Ser¹⁰⁵ by RSK1 (Buck et al., 1999). In cells transfected with the plasmid encoding truncated or kinase-dead mutant form of RSK1, oltipraz failed to induce Ser¹⁰⁵ phosphorylation of C/EBP or GSTA2 gene transactivation. Thus, Ser¹⁰⁵ phosphorylation of 35-kDa C/EBP seemed to be mediated by RSK1. C/EBP is phosphorylated by other cellular kinases including PKC, protein kinase A, and Ras-MAPK (Nakajima et al., 1993; Trautwein et al., 1993, 1994; Buck et al., 1999; Hanlon et al., 2001). PKC may phosphorylate rat C/EBP at the residue of Ser¹⁰⁵, whereas MAPK downstream from Ras phosphorylates Thr²³⁵ of human C/EBP (analogous to Thr¹⁸⁹ in the rat form). GF109203 (PKC inhibitor) did not inhibit the Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz (Supplemental Data S3). Oltipraz's increase in Ser¹⁰⁵-phosphorylated C/EBP was also unaffected by pretreatment with rapamycin (inhibitor of p70 ribosomal S6-kinase, 100 µM) or H89 (protein kinase A inhibitor, 20 µM) (data not shown) but weakly inhibited by Akt inhibitor IV (Supplemental Data S3). Thus, the possibility that Akt affects C/EBP phosphorylation was not completely excluded, although oltipraz failed to stimulate Akt (Kang et al., 2003).

We showed that RSK1 regulated Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz in primary rat hepatocytes as well as H4IIE cells. In addition, oltipraz enhanced phosphorylation of the mouse or human C/EBP at the residue of Thr²¹⁷ or Thr²⁶⁶, which was also catalyzed by RSK1. The role of human RSK1 in C/EBP-mediated gene activation was additionally supported by the RSK1 knockdown experiment. Our results provide evidence that activation of the C/EBP forms by oltipraz involves functionally analogous phosphorylation at the specific residues by RSK1 in the species. Oltipraz activation of C/EBP by specific phosphorylation would result in a conformational change of the protein for DNA binding and gene transactivation. The finding that oltipraz activates C/EBP via RSK1 brings insights into the role of organic compounds in activating the critical signaling pathway and cellular functions.

We showed previously that the pathways of MAPKs, ERK1/2, p38 kinase, and c-Jun N-terminal kinase, were not responsible for C/EBP-mediated glutathione S-transferase induction by oltipraz (Kang et al., 2003). In the current study, the extent of increase in Ser¹⁰⁵ phosphorylation of C/EBP or C/EBP-mediated gene transactivation by oltipraz in MKK1(-) cells was almost comparable with that in control, suggesting that oltipraz was capable of stimulating the Ser¹⁰⁵ phosphorylation independent of MKK1-ERK activity. In general, activation of ERK1/2 is necessary for RSK1 activation by epidermal growth factor (Roux et al., 2003). The observations that Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz was minimally decreased by chemical inhibition of MKK1-ERK1/2 and that RSK1 regulated Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz indicate that RSK1 activation elicited by oltipraz may not need the constitutive activity of ERK1/2. This is consistent with our previous observation that oltipraz did not enhance the activity of MAPK and that activation of C/EBP by oltipraz was independent of the ERK activity.

RSK1 activation by growth factors requires ERK docking near the C-terminal region (Roux et al., 2003). Ser³⁸⁰ phosphorylation of RSK1 (i.e., autophosphorylation) is catalyzed by the C-terminal kinase domain of activated RSK1. Activation of ERK initiates a series of activating processes of RSK1 (Roux et al., 2003), which includes autophosphorylation of RSK1. We found that the RSK1 levels in cell lysates were unchanged after oltipraz treatment. In additional experiments, we observed that Ser³⁸⁰ phosphorylation in RSK1 was increased by oltipraz treatment, which was persistent for up to 24 h. Increase in RSK1 kinase activity by oltipraz paralleled that in the Ser³⁸⁰ phosphorylation. It has been reported that RSK1 activated by epidermal growth factor or gonadotropin-releasing hormone translocates into the nucleus (Roux et al., 2003; Shah et al., 2003). After oltipraz treatment, RSK1, which was present in the cytoplasm under the resting condition, localized in the nucleus. Taken together, our data support that oltipraz treatment results in activation of RSK1. In contrast to the activation of MAPKs and PI3-kinase by mitogens, oltipraz did not increase the activities of the kinases (Kang et al., 2003). No change in oltipraz-inducible Ser¹⁰⁵ phosphorylation of C/EBP by MKK1-ERK1/2 inhibition supports that the mechanistic basis of RSK1 activation by oltipraz may differ from that by growth factors. The molecular basis as to how oltipraz activates RSK1 remains to be elucidated.

The PI3-kinase pathway affects cell growth, survival, and motility. We showed previously that PI3-kinase regulates C/EBP translocation, thus controlling C/EBP activation in response to oltipraz or hepatocyte growth factor (Cho and Kim, 2003b; Kang et al., 2003). In this study, we found for the first time that oltipraz increased the activity of RSK1 for C/EBP activation and that increases in the metabolic activity and nuclear translocation of RSK1 were dependent on the PI3-kinase activity. In general, full activation of RSK1 requires phosphorylation by PDK1, a constitutively active kinase downstream of PI3-kinase (Casamayor et al., 1999; Richards et al., 2001). After activation by PDK1, RSK1 translocates to the nucleus (Shah et al., 2003). In the current study, PI3-kinase inhibition prevented nuclear translocation of RSK1 by oltipraz. We observed that either chemical inhibition of PI3-kinase or stable transfection with the plasmid encoding p85 regulatory subunit almost completely inhibited an increase in Ser¹⁰⁵-phosphorylated C/EBP in the nuclear fraction, which was in line with the PI3-kinase dependence of RSK1 activation. It is likely that interruption of C/EBP activation by PI3-kinase inhibition, presumably through PDK1 (and/or Akt) inhibition, results from no phosphorylation in C/EBP at Ser¹⁰⁵. This is consistent with our previous observation (Kang et al., 2003) and also with the report that full RSK1 activation requires PDK1 activity downstream from PI3-kinase. The data indicate that RSK1-mediated Ser¹⁰⁵ phosphorylation of C/EBP by oltipraz requires the constitutive activity of PI3-kinase.

In conclusion, oltipraz induces phosphorylation of rat C/EBP form at Ser¹⁰⁵ and the mouse and human forms at Thr^217/266. RSK1 activation by oltipraz, which is dependent on PI3-kinase but not ERK1/2, contributes to the specific phosphorylation of C/EBP that leads to recruitment of CBP coactivator for GSTA2 gene transactivation. RSK1-mediated phosphorylation of C/EBP at specific residues by a pharmacological agent and its gene transactivation holds a significant implication for the molecular target of oltipraz.

	Footnotes

 
This work was supported by the National Research Laboratory Program (2001-2006), Korea Science and Engineering Foundation, The Ministry of Education, and Human Resources Development, the Republic of Korea.

Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org.

doi:10.1124/mol.105.018465.

ABBREVIATIONS: RSK, p90-ribosomal S6-kinase; C/EBP, CCAAT/enhancer binding protein-; ChIP, chromatin immunoprecipitation; CTT-RSK, C-terminal truncated-p90-ribosomal S6-kinase; ERK, extracellular signal-regulated kinase; MAPK, mitogen-activated protein kinase; PDK1, 3-phosphoinositide-dependent protein kinase-1; PI3, phosphatidylinositol 3; CBP, cAMP response element-binding protein binding protein; bp, base pair(s); HA, hemagglutinin; FCS, fetal calf serum; PCR, polymerase chain reaction; siRNA, small-interference RNA; scRNA, scrambled RNA; PKC, protein kinase C; GF109203, bisindoly-maleimide; U0126, 1,4-diamino-2,3-dicyano-1,4-bis(methylthio)butadiene; LY294002, 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride; H89, N-[2-(4-bromocinnamylamino)ethyl]-5-isoquinoline.

The online version of this article (available at http://molpharm.aspetjournals.org) contains supplemental material.

Address correspondence to: Dr. Sang Geon Kim, College of Pharmacy, Seoul National University, Sillim-dong, Kwanak-gu, Seoul 151-742, South Korea. E-mail: sgk{at}snu.ac.kr

	References

Top Abstract Materials and Methods Results Discussion References

 
Bhatt RR and Ferrell JE Jr (1999) The protein kinase p90 RSK as an essential mediator of cytostatic factor activity. Science (Wash DC) 286: 1362-1365.[Abstract/Free Full Text]

Bolton MG, Munoz A, Jacobson LP, Groopman JD, Maxuitenko YY, Roebuck BD, and Kensler TW (1993) Transient intervention with oltipraz against aflatoxin-induced hepatic tumorigenesis. Cancer Res 53: 3499-3504.[Abstract/Free Full Text]

Buck M and Chojkier M (2003) Signal transduction in the liver: C/EBP modulates cell proliferation and survival. Hepatology 37: 731-738.[CrossRef][Medline]

Buck M, Poli V, Hunter T, and Chojkier M (2001) C/EBP phosphorylation by RSK creates a functional XEXD caspase inhibitory box critical for cell survival. Mol Cell 8: 807-816.