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Subunit Signaling Inhibits Neutrophil Chemotaxis and InflammationDepartments of Pharmacology and Physiology (D.M.L., A.V.S.), and Pathology (A.M.P.B.S.), University of Rochester, Rochester, New York
Received September 14, 2007; accepted November 13, 2007
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Abstract |
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subunit-dependent signaling is important for chemoattractant-dependent leukocyte chemotaxis. Selective small molecule targeting of phosphoinositide 3-kinase (PI3-kinase) catalytic activity is a target of interest for anti-inflammatory pharmaceutical development. In this study, we examined whether small-molecule inhibition of Gβ-dependent signaling, including Gβ-dependent activation of PI3-kinase and Rac1, could inhibit chemoattractant-dependent neutrophil migration in vitro and inflammation in vivo. Small-molecule Gβ inhibitors suppressed fMLP-stimulated Rac activation, superoxide production, and PI3-kinase activation in differentiated HL60 cells. These compounds also blocked fMLP-dependent chemotaxis in HL60 cells and primary human neutrophils. Systemic administration inhibited paw edema and neutrophil infiltration in a mouse carrageenan-induced paw edema model. Overall, the data demonstrate that targeting Gβ-regulation may be an effective anti-inflammation strategy.
, Gβ, and G subunits. Ligand binding to receptors catalyzes the exchange of tightly bound GDP for GTP on the G subunit, liberating it from the Gβ subunits. Dissociation of the G and Gβ subunits can allow each to directly bind to downstream effector proteins (Gilman, 1987
; Oldham and Hamm, 2006). The free Gβ subunits released from Gi heterotrimers upon chemoattractant receptor activation initiate critical signaling pathways to direct chemoattractant-dependent neutrophil functions including chemotaxis and superoxide production (Neptune and Bourne, 1997).
Key direct targets of Gβ subunit binding and activation in neutrophils are phosphoinositide 3-kinase (PI3-kinase ) (Stephens et al., 1994, 1997; Stoyanov et al., 1995), Phospholipase C β (PLCβ) (Wu et al., 2000), and P-Rex (Welch et al., 2002). PI3-kinase has been noted to be a central mediator of chemotaxis and plays a pivotal role in leukocyte recruitment to inflamed tissues (Hirsch et al., 2000; Li et al., 2000; Camps et al., 2005). PIP3, produced by PI3-kinase catalytic activity, is critical to the development of cell polarity, which is necessary for chemokine-mediated cell motility and directional sensing (Wu et al., 2000). PI3-kinase -deficient neutrophils have impaired responses to various chemoattractants, including diminished chemotaxis (Hirsch et al., 2000; Li et al., 2000) and respiratory burst (Li et al., 2000; Sasaki et al., 2000), in response to GPCR activation. Small-molecule inhibitors of PI3-kinase catalytic activity have been demonstrated to suppress joint inflammation in mouse models of inflammation (Barber et al., 2005; Camps et al., 2005). Critical to the success of a method that targets PI3-kinase activity as a therapeutic anti-inflammatory approach is development of selective inhibitors that do not target other PI3-kinase isoforms, because these enzymes are critically involved in multiple aspects of mammalian cell function (Rückle et al., 2006).
In this study, we demonstrate a novel strategy to inhibit chemoattractant-dependent chemotaxis and inflammation using recently identified compounds that block Gβ-interactions with effectors, including PI3-kinase , by binding to a protein-protein interaction "hot spot" on the Gβ subunit (Bonacci et al., 2006). We show that these compounds block fMLP-dependent PI3-kinase activation, Rac1 activation, superoxide production, and neutrophil migration in vitro. Furthermore, when administered in vivo, neutrophil-dependent inflammation is inhibited, demonstrating that suppressing key Gβ-dependent signaling functions with small molecules has significant anti-inflammatory potential.
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Materials and Methods |
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Competition ELISA and Structure Activity Relationships. Binding of small molecules to Gβ12 was assessed by competition with phage displaying the SIGK peptide as described previously (Scott et al., 2001; Bonacci et al., 2006). In brief, Gβ12 (25 nM), with biotin incorporated via an N-terminal acceptor peptide on Gβ1, was immobilized in a 96-well plate coated with streptavidin. Compounds/DMSO and 0.1 x 1010 phage were added simultaneously and subsequently incubated for 1 h at room temperature. Plates were then washed with 1x Tris-buffered saline/0.5% Tween 20 and incubated with anti-M13, HRP-conjugated antibody (Amersham, Chalfont St. Giles, Buckinghamshire, UK). Phage binding was determined by monitoring A405 upon addition of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (Sigma Aldrich).
Surface Plasmon Resonance. Direct binding of "hot spot" binding small molecules was assessed using the Reichert SR7000 Surface Plasmon Resonance dual chamber instrument equipped with an autosampler (Reichert, Depew, NY). To activate the sensor chip surface, a mixture of 0.1 M 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 0.05 M N-hydroxysuccinimide was injected (flow rate 5 µl/min) over the sensor surface. Streptavidin (100 µg/ml; prepared in 20 mM sodium acetate buffer, pH 5.5) was coupled to the sensor chip surface followed by quenching the remaining activated carboxyl groups with 1 M ethanolamine, pH 8.5. bGβ12 was conjugated to the streptavidin-coated chip in running buffer (50 mM HEPES, pH 7.6, 1 mM EDTA, 100 mM NaCl, 0.1% C12E10, and 1 mM dithiothreitol) to achieve 2000 micro-refractive index units. A second reference cell was treated similarly, but bGβ12 was excluded. Direct binding of small molecules was tested out at room temperature with a flow rate of 75 µl/min. Compounds were prepared in running buffer and injected for 10 min followed by a dissociation phase of 10 min. The obtained sensorgrams were corrected for nonspecific background by running the same experiment in series over an identical sensor surface with immobilized streptavidin, blocked with biotin, without bGβ. Binding rates and constants were independent of flow rate over a wide range and did not fit mass transport limited models, indicating that mass transport was not limiting association or dissociation rates. Best fit kinetic parameters were determined using kinetic titration model and ClampXP ver. 3.5, which accounts for incomplete dissociation between injections (Karlsson et al., 2006).
GFP-PH-Akt Translocation in Differentiated HL60 Cells. HL60 cells stably overexpressing GFP-PH-Akt (Servant et al., 2000) were maintained and differentiated as described previously (Bonacci et al., 2006). Cells (0.2 x 106 cells/ml) were differentiated by incubation with 1.2% DMSO for 5 days. Cells were washed with serum-free RPMI 1640 (Invitrogen, Carlsbad, CA). Cells (2 x 105; 100 µl) were transferred to a 1.8-ml Beckman ultracentrifuge tube. Cells were pretreated with DMSO or compounds for 10 min at room temperature and stimulated with 250 nM fMLP (Sigma Aldrich) for 2 min. at 37°C, snap-frozen in liquid nitrogen, and thawed in the presence of 100 µl of 2x lysis buffer (100 mM HEPES, pH 8.0, 6 mM MgCl2, 0.2 mM EDTA, 200 mM NaCl, 100 µMNa3VO4, and protease inhibitors). Membranes were harvested by centrifugation 100,000g for 20 min after four freeze-thaw cycles. All treatments contained the same final concentration of DMSO. Pellets were washed once with 100 µl of lysis buffer (50 mM HEPES, pH 8.0, 3 mM MgCl2, 0.1 mM EDTA, 100 mM NaCl, 50 µM Na3VO4, and protease inhibitors), pelleted as above, and boiled in 2x sample loading buffer. Samples were resolved by 12% SDS-PAGE, transferred to nitrocellulose, and probed with anti-GFP antibody (1:1000; Roche) followed by incubation with goat anti-mouse HRP-conjugated secondary antibody (1: 5000, Bio-Rad Laboratories, Hercules, CA). Chemiluminescence was analyzed using a charge-coupled device camera in a UVP Epi-Chem II Darkroom imaging system. All samples had background subtracted and were normalized to the fMLP-induced signal set at 100%.
Cell Motility Analysis. Chemotaxis was assayed using a Boyden chamber (Neuro Probe, Gaithersburg, MD) using 3-µm polyvinylpyrrolidone-free polycarbonate filters (Neuro Probe). HL60 cells (differentiated for 4 days) were washed with and resuspended in HBSS containing 1% bovine serum albumin to a final concentration of 1 x 106 cells/ml. Primary human neutrophils were washed and resuspended in NaCl buffer (140 mM NaCl, 4 mM KCl, 10 mM D-glucose, 10 mM HEPES, pH 7.4, 1 mM MgCl2, and 1 mM CaCl2) to a final concentration of 1 x 106 cells/ml. Chemoattractant [1 µM granulocyte-macrophage-colony stimulating factor (GM-CSF), 10 nM IL-8, or 250 nM fMLP; Sigma Aldrich] was added to the bottom chamber in HBSS containing 1% bovine serum albumin. Cell suspensions (0.2 x 106 cells/well) were added to the top wells of the Boyden chamber and allowed to migrate for 1 h at 37°C. When applicable, cell suspensions were preincubated for 10 min with small-molecule inhibitors (in DMSO) at the indicated concentrations, and the bottom chamber was adjusted to the same concentration of small molecule. All treatments contained the same final concentration of DMSO. Filters were processed according to the manufacture's recommendations and stained using DifQuik (VWR Scientific, West Chester, PA). Chemotactic HL60 cells were scored by counting three microscope fields and subtracting the number of cells from fMLP wells as background. All samples had background subtracted and were normalized to the fMLP-induced signal set at 100%. For all the small molecules, effects of the compounds on chemokinesis was analyzed by measuring chemotaxis in the presence of 250 nM fMLP in both the upper and lower chambers of the Boyden Chamber and measuring changes in transwell migration. Unless otherwise indicated, none of the compounds had effects on chemokinesis.
Measurement of Superoxide Production. The nitro blue tetrazolium (NBT) method was used to assess the effects of Gβ inhibitors on NADPH oxidase activity. HL60 cells (differentiated for 4 days) were washed with and resuspended in HBSS containing calcium and magnesium (Cellgro; Mediatech, Herndon VA) to a final concentration of 2 x 106 cells/ml. Cells (1 x 106/reaction) were pretreated with 10 µMGβ inhibitor compound (in DMSO) or 100 nM wortmannin (Sigma Aldrich) for 10 min at 37°C before the addition of NBT (25 µl of 10 mg/ml in methanol) and then incubated or an additional 5 min at 37°C. All treatments contained the same final concentration of DMSO. Cells were then activated with either 250 nM fMLP (Sigma Aldrich) or PMA (Sigma Aldrich) for 30 min in a 37°C water bath. Reactions were stopped by the addition of 500 µl of 1.2 N HCl, and cells were collected by centrifugation at 12,000g for 5 min. Cell pellets were then resuspended in 200 µl of DMSO, transferred to a 96-well plate, and absorbance was measured at 540 nM. All treatments and controls contained the same concentration of DMSO. All samples had background subtracted and were normalized to the fMLP-induced signal set at 100%.
Evaluation of Rac-1 Activation. HL60 cells (differentiated for 4 days) were washed with and resuspended in NaCl buffer to a final concentration of 20 x 106 cells/ml. Cells (10 x 106/reaction) were pretreated with 10 µMGβ inhibitor (in DMSO) for 10 min before challenge with 1 µM fMLP for 90 s in a 37°C water bath and then immediately transferred to an ice-water bath. Cells were recovered by centrifugation at 500g and washed two times with ice-cold Tris-buffered saline, pH 7.4. The Rac1 activation assay kit (Upstate Cell Signaling Solutions, Billerica, MA) was used to prepare cell extracts and evaluate Rac1 activation according to the manufacturer's instructions. Affinity-purified GTP-Rac1 was resolved by 15% SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose, probed with anti-Rac1 monoclonal antibody followed by detection with HRP-conjugated secondary antibody in accordance with the manufacturer's recommendations. Chemiluminescence was analyzed using a charge-coupled device camera in a UVP Epi-Chem II Darkroom imaging system. Results were expressed as percent fMLP-stimulated GTP Rac1 normalized to total Rac1 with the background subtracted.
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Paws were amputated 2 h after carrageenan injection to determine the number of neutrophils present in the edematous fluid. Paws were transferred to prepared Eppendorf tubes, and the exudates were collected by centrifugation for 2 min. The paws were removed and the tubes re-weighed to determine the mass of the exudates, which was then converted to volume. The volume in the contralateral untreated control paw was subtracted from the cardgeenan-treated paw volume. To remove erythrocytes by hypertonic lysis, the exudate was resuspended in 250 µl of 1x PBS to which 50 µl of water was added. At the conclusion of a 30-s incubation, 75 µl of 4.5x PBS was added to return the solution to normal salt levels. The number of neutrophils present was determined by manual counting, and the small number of neutrophils in the control contralateral paw was subtracted.
Isolation of Primary Human Neutrophils. Human blood obtained by venous puncture from consenting, healthy adult donors, in accordance with University of Rochester standards, and collected in sterile vacutubes containing sodium heparin (BD Biosciences, San Jose, CA). Neutrophils were layered over PolymorphprepT11 (Accurate Chemical and Scientific Co., Westbury, NY) and isolated by centrifugation (470g for 50 min at room temperature). Trace erythrocytes were removed by hypertonic treatment followed by centrifugation. Isolated neutrophils were stored in HEPES-buffered saline solution (146 mM NaCl, 5 mM KCl, 5.5 mM D-glucose, 10 mM HEPES, 1 µM CaCl2, and 1 mM MgSO4) at pH 7.4.
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Results |
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. Previous studies identified multiple compounds that blocked effector binding to Gβ. A lead compound, M119, inhibited Gβ-dependent PI3-kinase and PLCβ activation in vitro and blocked chemoattractant/PI3-kinase-dependent GFP-PH-Akt translocation to membranes as well as Ca2+ release (Bonacci et al., 2006). We identified a related compound, gallein, that differs from M119 by the substitution of a benzene carboxylic acid for cyclohexane carboxylic acid at the 9 position of the core xanthene (Fig. 1A). Gallein is commercially available at high purity as a single isomer and in quantities necessary for in vivo analysis, so the Gβ binding properties of gallein were compared with M119. Gallein effectively competed for binding of SIGK peptide to Gβ in a phage ELISA assay with an IC50 comparable with that of M119 (Fig. 1B). We have shown previously that compounds that block peptide binding in this assay are effective competitors of many Gβ protein-protein interactions.
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, surface plasmon resonance (SPR) measurements were performed with streptavidin-immobilized biotinylated Gβ12. Binding and dissociation of gallein was monitored as a function of concentration and time. Data were fit to one site association and dissociation models to calculate association and dissociation rate constants from which affinity constants were derived (Fig. 1C, Table 1). Based on the SPR analysis, gallein bound to Gβ12 (Fig. 1C) with a Kd value of approximately 400 nM (Table 1), in relatively close agreement with the IC50 of 200 nM observed in the competition ELISA assay. The control compound, fluorescein, which did compete for SIGK binding in the competition ELISA, did not have detectable binding by SPR (Table 1). It is noteworthy that binding and dissociation rates for gallein were relatively slow (Fig. 1C). These data confirm that gallein binds directly to Gβ, and resulting effects on competition for effector binding are likely to result from direct binding to Gβ with high affinity. Based on these data, gallein would be predicted to have similar effects to M119 when tested in in vitro and in vivo assays.
Inhibition of G Protein-Dependent Chemotactic Peptide Signaling with Small Molecules. A key molecule involved in chemotactic peptide signaling is PI3-kinase . To determine whether gallein, like M119, modulates the receptor-dependent activation of PI3-kinase, HL60 cells expressing GFP-PH-Akt were pretreated with compound and challenged with fMLP. Gallein inhibited fMLP-dependent GFP-PH-Akt translocation in differentiated HL60 cells with an efficacy comparable with that of M119 (Fig. 2A).
Activation of Rac in neutrophils is critical for fMLP-dependent activation of NADPH-oxidase and subsequent superoxide production and is dependent on Gβ- and PIP3-dependent activation of the Rac-specific exchange factor P-Rex. Other previous work showed that M119 inhibited P-Rex1 activation by fMLP suggesting the compounds could inhibit Rac activation (Zhao et al., 2007). We determined whether Gβ-binding compounds would inhibit receptor-dependent activation of Rac1. Rac1GTP levels were measured in cytosolic extracts of HL60 cells that had been pretreated with compounds before stimulation with fMLP. M119 and gallein inhibited fMLP-induced Rac1 activation in differentiated HL60 cells, whereas the negative control compound M119B had little effect (Fig. 2B).
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Inhibitors Blocked fMLP-Dependent Superoxide Production and Neutrophil Chemotaxis. The ability of these small molecules to block fMLP-induced chemotaxis and superoxide production was assessed to determine whether the compounds could block relevant cellular functions downstream of PI3-kinase and Rac. Both of these functions are critical to the inflammatory process and inhibition of both processes could contribute to anti-inflammatory effects of Gβ inhibitors.
M119 and gallein both significantly inhibited fMLP-dependent superoxide production. Control compounds M119B and fluorescein did not significantly affect this response (Fig. 2C). Wortmannin also blocked fMLP-dependent superoxide production, indicating that the pathway was a PI3-kinase-dependent pathway. We also examined PMA-dependent superoxide production, a process not dependent on Gβ. PMA-dependent superoxide production was not inhibited by any of the Gβ binding compounds or controls (Fig. 2D), indicating that the compounds specifically inhibited the Gβ-dependent pathway to superoxide production.
fMLP-dependent chemotaxis was assessed in a Boyden Chamber. M119 and gallein inhibited fMLP-induced chemotaxis in differentiated HL60 cells with similar efficacy (Fig. 3A). Neither M119 nor gallein had any effects on chemokinesis measured in the transwell assay with fMLP in both the upper and lower chambers (data not shown). To support the idea that the mode of action of these compounds is dependent on Gβ heterodimers liberated from Gi-coupled chemokine receptors, cells were challenged with GM-CSF. Chemotaxis induced by GM-CSF is partly dependent on PI3-kinase activity in human neutrophils but is independent of Gβ-stimulation of PI3-kinase (Gomez-Cambronero et al., 2003). If the small molecules were acting directly on PI3-kinase or by a nonspecific mechanism, they would be expected to block GM-CSF-induced chemotaxis. Neither M119 nor gallein blocked GM-CSF-induced chemotaxis in differentiated HL60 cells (Fig. 3B). These data demonstrate two key points: 1) the general chemotaxis machinery in HL60 cells is not affected by these compounds and 2) the compounds selectively inhibited GPCR-dependent chemotaxis, consistent with inhibition of Gβ signaling.
The inhibitory properties of these compounds in potentially more clinically relevant isolated primary human neutrophils were also evaluated. Again, both M119 and gallein significantly inhibited fMLP-induced chemotaxis (Fig. 3C). Wortmannin, a general PI3-kinase inhibitor (Okada et al., 1994), also blocked chemotaxis (Fig. 3C). Conversely, M119B binds only weakly to the Gβ "hot spot" (Table 1 in Bonacci et al., 2006) and had no affect on cell motility (Fig. 3C), suggesting that the observed effects on chemotaxis are dependent on small-molecule-binding to the Gβ "hot spot." IL-8 regulated chemotaxis was also blocked by M119 and gallein (Fig. 3C) extending our findings to other Gi coupled chemoattractants and supporting the idea that the mechanism of action of these compounds is to inhibit Gβ subunit signaling down-stream of chemoattractant/chemokine receptors.
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and block Gβ-dependent PI3-kinase regulation in vitro and in HL60 cells potently inhibit GPCR-dependent neutrophil chemotaxis with an IC50 comparable with what has been published for direct PI3-kinase catalytic inhibitors on chemokine-dependent monocyte chemotaxis (Camps et al., 2005).
Gallein Attenuates Inflammation and Neutrophil Recruitment in Vivo. Inhibition of chemoattractant-dependent chemotaxis of neutrophils would be predicted to inhibit neutrophil-dependent inflammation based on data from PI3-kinase knock-out mice (Hirsch et al., 2000; Li et al., 2000). To assess the in vivo efficacy of Gβ-binding small molecules in blocking neutrophil chemotaxis we tested gallein in the carrageenan paw edema model. Carrageenan, when injected into the glabrous tissue of the hind paw, leads to rapid acute inflammation characterized by infiltration of neutrophils (Siqueira-Junior et al., 2003). Gallein or vehicle control was delivered by intraperitoneal injection 1 h before injection of carrageenan into the paw. Peak paw edema was observed 2 h after carrageenan injection (Fig. 4A). Indomethacin, a nonselective nonsteroidal anti-inflammatory drug that inhibits cyclooxygenases 1 and 2 (Siqueira-Junior et al., 2003), is the drug of choice for comparative in vivo efficacy studies. Pretreatment with indomethacin sharply reduced paw edema. It is noteworthy that prophylactic administration of 100 mg/kg gallein reduced paw edema to levels comparable with that of indomethacin. Injection with gallein in the absence of carrageenan had no effect on paw thickness (data not shown). Additional experimentation determined that the ED50 value of gallein for inhibiting paw edema to be approximately 20 mg/kg (Fig. 4B). Acute phase inflammation, as seen in the carrageenan-induced paw edema model, is characterized by neutrophil infiltration (Siqueira-Junior et al., 2003; Posadas et al., 2004). To confirm that neutrophil infiltration was inhibited, the number of neutrophils in paw exudates was quantified. Pretreatment with 100 mg/kg gallein reduced the number of neutrophils in the edematous fluid by approximately half to levels comparable with that seen with indomethacin (Fig. 4C). These data correlated well with the actual volume of exudate fluid. Pretreatment with indomethacin and gallein also reduced exudate volume by approximately 75% (Fig. 4D).
Oral administration of gallein (30 mg/kg) in mice 1 h before carrageenan challenge also significantly reduced paw swelling by 40% comparable with that of indomethacin (Fig. 5). These data demonstrate that gallein is absorbed into systemic circulation, is bioavailable, and systemically impairs neutrophil recruitment. To support the conclusion that the observed reduction in paw swelling was Gβ-dependent, we tested the M119B-like compound fluorescein under identical experimental conditions. Fluorescein differs from M119B only by the substitution of an aromatic benzene ring for a cyclohexane at the 9 position of the core xanthene (Fig. 1A) and binds Gβ very weakly if at all (Table 1). Fluorescein did not reduce paw swelling under identical experimental conditions (Fig. 5), which indicates that the observed reduction in paw swelling and neutrophil infiltration was correlated with the ability of the small molecules to bind to Gβ.
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Discussion |
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subunits and effectors that are critical for neutrophil migration in response to activation of chemoattractant receptors. Gβ-responsive PI3-kinase production of PIP3 is critical to neutrophil functions, including superoxide production, chemotaxis, and cellular polarization (Hirsch et al., 2000; Li et al., 2000). We have shown that Gβ-binding small molecules inhibit interactions between Gβ and PI3-kinase . These same molecules block PI3-kinase and Rac1 activation in HL60 cells, chemoattractant-dependent superoxide production and chemotaxis in differentiated HL60 cells. These findings were extended to inhibition of chemoattractant-dependent chemotaxis in primary human neutrophils and ultimately neutrophil-dependent inflammation in vivo.
PI3-kinases play diverse roles in normal cellular physiology, including cell motility and survival (Cantley, 2002). Camps and colleagues identified and characterized small-molecule inhibitors of PI3-kinase that are competitive with ATP (Camps et al., 2005). These small molecules were efficacious in mouse models of rheumatoid arthritis and systemic lupus, providing further rationale for pharmacological targeting of the pathway as therapeutic strategy (Barber et al., 2005; Camps et al., 2005). However, a concern with inhibitors of this class is that because many kinases of the same family have significant homology, there may be difficulty in developing inhibitors that are selective for PI3-kinase , although some progress has been made in this area (Rückle et al., 2006). The strategy presented here selects for a single isoform of the PI3-kinase family because PI3-kinase is the only PI3-kinase isoform that uses Giβ-dependent activation as a major mechanism for regulation (Stephens et al., 1994, 1997; Hirsch et al., 2000).
Compounds such as M119 and gallein also inhibit interactions between Gβ and other targets that are critical for chemoattractant-dependent directed migration or reactive oxygen species production by neutrophils or other monocytes. For example, M119 blocks membrane translocation of P-Rex, a PIP3- and Gβ-regulated Rac2 exchange factor, in human neutrophils, which could be due in part to directly blocking P-Rex binding to Gβ in addition to blocking PIP3 production by PI3-kinase (Zhao et al., 2007). It has recently been shown that Ras is required for full PI3-kinase activation in neutrophils (Suire et al., 2006). It is possible that the inhibitors block Gβ-dependent activation of Ras and in part act to block PI3-kinase activation in cells indirectly through inhibition of Ras activation. In Dictyostelium discoideum, both PI3-kinase and phospholipase A2 activities downstream of G protein activation are important for chemotaxis (Chen et al., 2007). Other studies have shown chemoattractant-dependent chemotaxis becomes PI3-kinase-independent under certain conditions (Ferguson et al., 2007). Thus, a broad-spectrum Gβ inhibitor could be more effective, in some cases, than selective PI3-kinase inhibitors because they can block other Gβ interactions besides PI3-kinase-, a property that could contribute to therapeutic efficacy. Nevertheless, because of the wide roles of Gβ in regulation of cell physiology, development of Gβ binding small molecules that are more selective for PI3-kinase inhibition relative to other Gβ-dependent pathways is an important direction.
Inflammation is a central mediator of many human conditions, including atherosclerosis, allergic reactions, psoriasis, virus-induced myocarditis, ischemia-reperfusion injury, and rheumatoid arthritis. The inflammatory process involves complex signaling cascades partly coordinated by chemokines, which recruit leukocytes, including large numbers of neutrophils, to sites of inflammation (Wu et al., 2000). Targeting chemokines with antibodies or binding proteins as well as targeting chemokine receptors has been attempted as a therapeutic strategy (Gong et al., 1997; Ogata et al., 1997; Plater-Zyberk et al., 1997; Barnes et al., 1998; Halloran et al., 1999; Matthys et al., 2001; Podolin et al., 2002; Yang et al., 2002) However, the overwhelming complexity of these signaling molecules (multiple chemokines, chemokine receptors, and redundancy) is a significant hurdle. Polychemokine (Carter, 2002) or combinations of different chemokine (al Mughales et al., 1996) antagonists have been suggested, but there may be chemokines that act as an agonist at one receptor and an antagonist at another (Xanthou et al., 2003). Despite this complexity, these chemokine receptors and ligands represent a tantalizing therapeutic target because of their integral role in the inflammatory process. Targeting PI3-kinase has been suggested as strategy for blocking a common signal downstream of chemokine receptors (Camps et al., 2005; Rückle et al., 2006). The data presented here indicate that targeting Gβ could also circumvent the redundancy of the chemokine system and may offer some advantages to direct targeting of PI3-kinase .
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Footnotes |
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ABBREVIATIONS: GPCR, G protein-coupled receptor; PI3, phosphoinositide 3; PLC, phospholipase C; P-Rex, phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchange factor; PIP3, phosphatidylinositol 3,4,5-trisphosphate; bGβ, N terminally biotinylated Gβ; fMLP, formyl-Met-Leu-Phe; ELISA, enzyme-linked immunosorbent assay; DMSO, dimethyl sulfoxide; HRP, horseradish peroxidase; HBSS, Hanks' balanced saline solution; GM-CSF, granulocyte-macrophage-colony stimulating factor; IL, interleukin; NBT, nitro blue tetrazolium; PMA, phorbol 12-myristate 13-acetate; GFP-PH-Akt, Green fluorescent protein fusion with the pleckstrin homology domain from Akt protein kinase; ANOVA, analysis of variance.
Address correspondence to: A. V. Smrcka, University of Rochester, Department of Pharmacology and Physiology, 601 Elmwood Ave., Box 711, Rochester, NY 14642. E-mail: alan_smrcka{at}urmc.rochester.edu
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References |
|---|
|
TopAbstractMaterials and MethodsResultsDiscussionReferences |
|---|
Barber DF, Bartolome A, Hernandez C, Flores JM, Redondo C, Fernandez-Arias C, Camps M, Rückle T, Schwarz MK, Rodriguez S, et al. (2005) PI3K inhibition blocks glomerulonephritis and extends lifespan in a mouse model of systemic lupus. Nat Med 11: 933-935.[Medline]
Barnes DA, Tse J, Kaufhold M, Owen M, Hesselgesser J, Strieter R, Horuk R, and Daniel Perez H (1998) Polyclonal antibody directed against human RANTES ameliorates disease in the Lewis rat adjuvant-induced arthritis model. J Clin Invest 101: 2910-2919.[Medline]
Bonacci TM, Mathews JL, Yuan C, Lehmann DM, Malik S, Wu D, Font JL, Bidlack JM, and Smrcka AV (2006) Differential targeting of Gβ-subunit signaling with small molecules. Science 312: 443-446.
Camps M, Rückle T, Ji H, Ardissone V, Rintelen F, Shaw J, Ferrandi C, Chabert C, Gillieron C, Francon B, et al. (2005) Blockade of PI3K suppresses joint inflammation and damage in mouse models of rheumatoid arthritis. Nat Med 11: 936-943.[Medline]
Cantley LC (2002) The phosphoinositide 3-kinase pathway. Science 296: 1655-1657.
Carter PH (2002) Chemokine receptor antagonism as an approach to anti-inflammatory therapy: `just right' or plain wrong? Curr Opin Chem Biol 6: 510-525.[CrossRef][Medline]
Chen L, Iijima M, Tang M, Landree MA, Huang YE, Xiong Y, Iglesias PA, and Devreotes PN (2007) PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis. Developmental Cell 12: 603-614.[CrossRef][Medline]
Ferguson GJ, Milne L, Kulkarni S, Sasaki T, Walker S, Andrews S, Crabbe T, Finan P, Jones G, Jackson S, et al. (2007) PI(3)K has an important context-dependent role in neutrophil chemokinesis. Nat Cell Biol 9: 86-91.[CrossRef][Medline]
Gilman AG (1987) G proteins: transducers of receptor-generated signals. Annu Rev Biochem 56: 615-649.[CrossRef][Medline]
Gomez-Cambronero J, Horn J, Paul CC, and Baumann MA (2003) Granulocyte-macrophage colony-stimulating factor is a chemoattractant cytokine for human neutrophils: involvement of the ribosomal P70 S6 kinase signaling pathway. J Immunol 171: 6846-6855.
Gong JH, Ratkay LG, Waterfield JD, and Clark-Lewis I (1997) An antagonist of monocyte chemoattractant protein 1 (MCP-1) inhibits arthritis in the MRL-Lpr mouse model. J Exp Med 186: 131-137.
Halloran MM, Woods JM, Strieter RM, Szekanecz Z, Volin MV, Hosaka S, Haines GK, III, Kunkel SL, Burdick MD, et al. (1999) The role of an epithelial neutrophil-activating peptide-78-like protein in rat adjuvant-induced arthritis. J Immunol 162: 7492-7500.
Hirsch E, Katanaev VL, Garlanda C, Azzolino O, Pirola L, Silengo L, Sozzani S, Mantovani A, Altruda F, and Wymann MP (2000) Central role for G protein-coupled phosphoinositide 3-kinase in inflammation. Science 287: 1049-1053.
Karlsson R, Katsamba PS, Nordin H, Pol E, and Myszka DG (2006) Analyzing a kinetic titration series using affinity biosensors. Anal Biochem 349: 136-147.[CrossRef][Medline]
Li Z, Jiang H, Xie W, Zhang Z, Smrcka AV, and Wu D (2000) Roles of PLCβ-2 and β-3 and PI3K in chemoattractant-mediated signal transduction. Science 287: 1046-1049.
Matthys P, Hatse S, Vermeire K, Wuyts A, Bridger G, Henson GW, De Clercq E, Billiau A, and Schols D (2001) AMD3100, a potent and specific antagonist of the stromal cell-derived factor-1 chemokine receptor CXCR4, inhibits autoimmune joint inflammation in IFN- receptor-deficient mice. J Immunol 167: 4686-4692.
Neptune ER and Bourne HR (1997) Receptors induce chemotaxis by releasing the β subunit of Gi, not by activating Gq or Gs. Proc Natl Acad Sci U S A 94: 14489-14494.
Ogata H, Takeya M, Yoshimura T, Takagi K, and Takahashi K (1997) The role of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of collagen-induced arthritis in rats. J Pathol 182: 106-114.[CrossRef][Medline]
Okada T, Sakuma L, Fukui Y, Hazeki O, and Ui M (1994) Blockage of chemotactic peptide-induced stimulation of neutrophils by wortmannin as a result of selective inhibition of phosphatidyl inositol 3-kinase. J Biol Chem 269: 3563-3567.
Oldham WM and Hamm HE (2006) Structural basis of function in heterotrimeric G proteins. Q Rev Biophys 39: 117-166.[CrossRef][Medline]
Plater-Zyberk C, Hoogewerf AJ, Proudfoot AEI, Power CA, and Wells TNC (1997) Effect of a CC chemokine receptor antagonist on collagen induced arthritis in DBA/1 mice. Immunol Lett 57: 117-120.[CrossRef][Medline]
Podolin PL, Bolognese BJ, Foley JJ, Schmidt DB, Buckley PT, Widdowson KL, Jin Q, White JR, Lee JM, Goodman RB, et al. (2002) A potent and selective nonpeptide antagonist of CXCR2 inhibits acute and chronic models of arthritis in the rabbit. J Immunol 169: 6435-6444.
Posadas I, Bucci M, Roviezzo F, Rossi A, Parente L, Sautebin L, and Cirino G (2004) Carrageenan-induced mouse paw oedema is biphasic, age-weight dependent and displays differential nitric oxide cyclooxygenase-2 expression. Br J Pharmacol 142: 331-338.[CrossRef][Medline]
Rückle T, Schwarz MK, and Rommel C (2006) PI3K inhibition: towards an `aspirin of the 21st century'? Nat Rev Drug Discov 5: 903-918.[CrossRef][Medline]
Sasaki T, Irie-Sasaki J, Jones RG, Oliveira-dos-Santos AJ, Stanford WL, Bolon B, Wakeham A, Itie A, Bouchard D, Kozieradzki I, et al. (2000) Function of PI3K in thymocyte development, T cell activation, and neutrophil migration. Science 287: 1040-1046.
Scott JK, Huang SF, Gangadhar BP, Samoriski GM, Clapp P, Gross RA, Taussig R, and Smrcka AV (2001) Evidence that a protein-protein interaction `hot spot' on heterotrimeric G protein β subunits is used for recognition of a subclass of effectors. EMBO J 20: 767-776.[CrossRef][Medline]
Servant G, Weiner OD, Hezmark P, Balla T, Sedat JW, and Bourne HR (2000) Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science 287: 1037-1040.
Siqueira-Junior JM, Peters RR, Brum-Fernandes AJ, and Ribeiro-do-Valle RM (2003) Effects of valeryl salicylate, a COX-1 inhibitor, on models of acute inflammation in mice. Pharmacol Res 48: 437-443.[CrossRef][Medline]
Stephens L, Smrcka A, Cooke FT, Jackson TR, Sternweis PC, and Hawkins PT (1994) A novel, phosphoinositide 3-kinase activity in myeloid-derived cells is activated by G-protein β-subunits. Cell 77: 83-93.[CrossRef][Medline]
Stephens LR, Erdjument-Bromage H, Lui M, Cooke F, Coadwell J, Smrcka AV, Thelen M, Cadwallader K, Tempst P, and Hawkins PT (1997) The Gβ sensitivity of a PI3K is dependent upon a tightly associated adaptor, P101. Cell 89: 105-114.[CrossRef][Medline]
Stoyanov B, Volinia S, Hanck T, Rubio I, Loubchenkov M, Malek D, Stoyanova S, Vanhaesebroeck B, Dhand R, Nurnberg B, et al. (1995) Cloning and characterization of g protein-activated human phosphoinositide-3 kinase. Science 269: 690-693.
Suire S, Condliffe AM, Ferguson GJ, Ellson CD, Guillou H, Davidson K, Welch H, Coadwell J, Turner M, Chilvers ER, et al. (2006) Gβs and the ras binding domain of P110 are both important regulators of PI3K Signalling in Neutrophils. Nat Cell Biol 8: 1303-1309.[CrossRef][Medline]
Welch HCE, Coadwell WJ, Ellson CD, Ferguson GJ, Andrews SR, Erdjument-Bromage H, Tempst P, Hawkins PT, and Stephens LR (2002) P-Rex1, a PtdIns(3,4,5)P3- and Gβ-regulated guanine-nucleotide exchange factor for Rac. Cell 108: 809-821.[CrossRef][Medline]
Wu D, Huang CK, and Jiang H (2000) Roles of phospholipid signaling in chemoattractant-induced responses. J Cell Sci 113: 2935-2940.[Abstract]
Xanthou G, Duchesnes CE, Williams TJ, and Pease JE (2003) CCR3 functional responses are regulated by both CXCR3 and its ligands CXCL9, CXCL10 and CXCL11. Eur J Immunol 33: 2241-2250.[CrossRef][Medline]
Yang YF, Mukai T, Gao P, Yamaguchi N, Ono S, Iwaki H, Obika S, Imanishi T, Tsujimura T, Hamaoka T, et al. (2002) A non-peptide CCR5 antagonist inhibits collagen-induced arthritis by modulating T cell migration without affecting anti-collagen T cell responses. Eur J Immunol 32: 2124-2132.[CrossRef][Medline]
Zhao T, Nalbant P, Hoshino M, Dong X, Wu D, and Bokoch GM (2007) Signaling requirements for translocation of P-Rex1, a key Rac2 exchange factor involved in chemoattractant-stimulated human neutrophil function. J Leukoc Biol 81: 1127-1136.
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125 cells fMLP + vehicle), and represented as the percentage of fMLP-treated control cells. ***, P < 0.001 ANOVA is statistically different from control. Data shown pooled from three independent experiments, each in duplicate, ± S.E.M. B, neither M119 nor gallein blocks 1 µM GM-CSF-induced chemotaxis in a Boyden chamber. Chemotaxis was quantified as above by subtracting out background cells (0-10 cells) in the absence of chemoattractant to obtain total transmigrated cells (
80% purity. Neutrophils (2 x 105) were pretreated with gallein (10 µM), M119 (10 µM), M119B (10 µM), or wortmannin (wtmn.) (1 µM) and then challenged with 250 nM fMLP or 10 nM IL-8 to evaluate chemotaxis in a Boyden chamber for 1 h at 37°C. Chemotaxis was quantified as above by subtracting out background cells (0-10 cells) to obtain total transmigrated cells (fMLP 
