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Tumor Necrosis Factor- Enhances Neutrophil Adhesiveness: Induction of Vascular Cell Adhesion Molecule-1 via Activation of Akt and CaM Kinase II and Modifications of Histone Acetyltransferase and Histone Deacetylase 4 in Human Tracheal Smooth Muscle Cells

Department of Physiology and Pharmacology, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (C.-W.L., H.-C.L., I.-T.L., C.-M.Y.); Department of Anesthetics, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (C.-C.L.); Department of Internal Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (S.-F.L.); Graduate Institute of Natural Products, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (T.-L.H.); and Department of Molecular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts (W.C.A.)

Received May 12, 2007; accepted January 23, 2008

	Abstract

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Up-regulation of vascular cell adhesion molecule-1 (VCAM-1) involves adhesions between both circulating and resident leukocytes and the human tracheal smooth muscle cells (HTSMCs) during airway inflammatory reaction. We have demonstrated previously that tumor necrosis factor (TNF)--induced VCAM-1 expression is regulated by mitogen-activated protein kinases, nuclear factor-B, and p300 activation in HTSMCs. In addition to this pathway, phosphorylation of Akt and CaM kinase II has been implicated in histone acetyltransferase and histone deacetylase 4 (HDAC4) activation. Here, we investigated whether these different mechanisms participated in TNF--induced VCAM-1 expression and enhanced neutrophil adhesion. TNF- significantly increased HTSMC-neutrophil adhesions, and this effect was associated with increased expression of VCAM-1 on the HTSMCs and was blocked by the selective inhibitors of Src [4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]-pyrimidine (PP1)], epidermal growth factor receptor [EGFR; 4-(3'-chloroanilino)-6,7-dimethoxy-quinazoline, (AG1478)], phosphatidylinositol 3-kinase (PI3K) [2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride(LY294002) and wortmannin],calcium[1,2-bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester; BAPTA-AM], phosphatidylinositol-phospholipase C (PLC) [1-[6-[[17β-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione (U73122 [GenBank] )], protein kinase C (PKC) [12-(2-cyanoethyl)-6,7,12, 13-tetrahydro-13-methyl-5-oxo-5H-indolo(2,3-a)pyrrolo(3,4-c)-carbazole (Gö6976), rottlerin, and 3-1-[3-(amidinothio)propyl-1H-indol-3-yl]-3-(1-methyl-1H-indol-3-yl) maleimide (bisindolylmaleimide IX) (Ro 31-8220)], CaM (calmidazolium chloride), CaM kinase II [(8R^*,9S^*,11S^*)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-14-n-propoxy-2,3,9, 10-tetrahydro-8,11-epoxy, 1H,8H, 11H-2,7b,11a-triazadibenzo[a,g]cycloocta[cde]trinden-1-one (KT5926) and 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN62)], p300 (curcumin), and HDAC (trichostatin A) or transfection with short interfering RNAs for Src, Akt, PKC, PKCµ, and HDAC4. At gene regulation level, reverse-transcriptase polymerase chain reaction and promoter assays revealed that expression of VCAM-1 was also attenuated by these signaling molecule inhibitors. Moreover, TNF- induced Akt and CaM kinase II phosphorylation via cascades through Src/EGFR/PI3K and PLC/calcium/CaM, respectively. Finally, activation of Akt and CaM kinase II may eventually lead to the acetylation of histone residues and phosphorylation of histone deacetylase. These findings revealed that TNF- induced VCAM-1 expression via multiple signaling pathways. Blockade of these pathways may be selectively targeted to reduce neutrophil adhesion via VCAM-1 suppression and attenuation of the inflammatory responses in airway diseases.

Besides these specific transcription factor-mediated signaling axes, general protein acetylation has been shown to influence a broad set of cellular responses, including diverse aspects of transcriptional regulation through the recruitment of two classes of enzymes, in particular histone acetyltransferases (HAT) and histone deacetylases (HDACs) (Roth et al., 2001). In patients with asthma, there is a marked increase in HAT and a small reduction in HDAC activities compared with those of normal airways (Barnes et al., 2005). The p300/CBP proteins are endowed with HAT activity, which transfers an acetyl group to the core histones of a lysine residue, and the acetylation level of chromatin has been established to be a key mechanism in regulating inflammatory gene transcription (Chan and La Thangue, 2001), such as VCAM-1 (Lee et al., 2006a), cyclooxygenase-2 (Nie et al., 2003), and matrix metalloproteinase-9 (Ma et al., 2005). Many studies have reported the regulation of p300 or CBP by protein kinases, including PKCs (Yuan and Gambee, 2000), CaMKIV (Impey et al., 2002), and p38 (Poizat et al., 2005). In addition, p300/CBP consisting of an RXRXXpS/T consensus sequence in the C terminus, where X is any amino acid, R is arginine, and pS/T is phosphorylated serine or threonine, preferred by Akt has been involved in TNF--induced ICAM-1 expression (Huang and Chen, 2005). Our current findings also suggest that upon TNF- treatment, p300 could be recruited to the VCAM-1 promoter, which may be regulated by these kinases.

Conversely, gene repression is mediated via HDACs, which catalyze deacetylation by cleaving acetyl groups, resulting in tightening of nucleosomal integrity and suppression of transcription (de Ruijter et al., 2003). There are multiple mammalian HDACs, which fall into three classes on the basis of structural and biochemical characteristics: class I deacetylases (HDACs 1, 2, 3, and 8), class II deacetylases include HDACs 4, 5, 6, 7, 9, and 10; and the third class of HDACs is the conserved nicotinamide adenine dinucleotide-dependent Sir2 family of deacetylases (Lin et al., 2006). Many transcription factors have been shown to associate with HDAC to regulate gene transcription. For instance, corticosteroids seem to suppress inflammation in asthma by switching off these inflammatory genes by targeting HDAC2. HDAC2 is able to deacetylate acetylated NF-B and promotes its association with the inhibitor IB- within the nucleus leading to export into the cytoplasm and thus terminates the activity of NF-B (Barnes et al., 2005). It is most interesting that class II HDACs possess the capability of activating nucleocytoplasmic shuttling, which are suggested to be regulated by CaMK-dependent phosphorylation. For instance, phosphorylation of HDAC4 by CaMKII promotes nuclear export and prevents nuclear import of HDAC4, with consequent derepression of HDAC4 targeting genes and results in hypertrophic growth (Liu et al., 2005; Backs et al., 2006; Little et al., 2007). In analogy, another study has proposed a novel mechanism in which CaMKIV mediates the phosphorylation of HDAC4, thus allowing IL-5 gene transcriptional activation (Han et al., 2006). These findings reveal a central role for HDAC4 in CaMK signaling pathways and have implications in the control of gene expression by calcium signaling in a variety of cell types.

In this presentation, we investigated the different signal intermediates that coupled TNF- to distinct subsets of target genes such as VCAM-1 in HTSMCs. We showed that TNF--mediated VCAM-1 induction was indeed governed by transcript-specific signaling pathways involving the Src/EGFR/PI3K and PLC/Ca²⁺/CaMK. Both pathways activate Akt and CaM kinase II and may eventually lead to the acetylation of histone residues and phosphorylation of histone deacetylase. Blockade of these pathways may selectively target to reduce neutrophil adhesion via VCAM-1 suppression and attenuation of the inflammatory response in airway diseases.

	Materials and Methods

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Reagents. DMEM/F-12 medium, fetal bovine serum, and TRIzol were purchased from Invitrogen (Carlsbad, CA). [-³²P]ATP (6000 Ci/mmol), Hybond C membrane, enhanced chemiluminescence (ECL) Western blotting detection system, and Hyperfilms were from GE Healthcare (Chalfont St. Giles, Buckinghamshire, UK). Polyclonal antibodies VCAM-1, HDAC4, and NF-B (p65) Ab were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-GAPDH antibody was from Biogenesis (Boumemouth, UK). Specific CaM Kinase II Assay kit was from Millipore (Billerica, MA). CaM kinase II, phospho CaM kinase II (Thr²⁸⁶), and phospho Akt Ab kits were from Cell Signaling Technology (Danvers, MA). PP1, AG1478, LY294002, wortmannin, curcumin, U73122 [GenBank] , BAPTA, KT5926, KN62, calmidazolium chloride, trichostatin A (TSA), Gö6976, Ro 31-8220, rottlerin, and MG132 were from BIOMOL Research Laboratories (Plymouth Meeting, PA). Bicinchoninic acid protein assay kit was from Pierce Chemical (Rockford, IL). Enzymes and other chemicals were from Sigma (St. Louis, MO).

Cell Culture. HTSMCs were purchased from ScienCell Research Lab (San Diego, CA) and cultured as described previously (Lee et al., 2004). When the cultures reached confluence, cells were treated with 0.05% (w/v) trypsin/0.53 mM EDTA for 5 min at 37°C. The cell suspension was diluted with DMEM/Ham's F-12 containing 10% fetal bovine serum to a concentration of 2 x 10⁵ cells/ml. The cell suspension was plated onto (1 ml/well) 12-well culture plates and (10 ml/dish) 10-cm culture dishes for the measurement of protein expression and mRNA accumulation, respectively. Experiments were performed with cells from passages three to eight.

Preparation of Cell Extracts and Western Blot Analysis. Growth-arrested HTSMCs were incubated with TNF- at 37°C for the indicated time. The cells were washed with ice-cold PBS, scraped, collected, and centrifuged at 45,000g for 1 h at 4 °C to yield the whole-cell extract, as described previously (Lee et al., 2004). Samples were denatured, subjected to SDS-PAGE using a 10% running gel, and transferred to nitrocellulose membrane. Membranes were incubated overnight at 4°C with an anti-VCAM-1, anti-HDAC4, anti-p65, anti-p50, or anti-GAPDH antibody used at a dilution of 1:1000 in 5% (w/v) BSA in Tween/Tris-buffered saline [(50 mM Tris-HCl, 150 mM NaCl, and 0.05% (w/v) Tween 20, pH 7.4)]. Membranes were incubated with a 1:2000 dilution of anti-rabbit or anti-mouse horseradish peroxidase antibody for 1 h. The immunoreactive bands detected by ECL reagents were developed by Hyperfilm-ECL.

Transfection of siRNAs for Src, Akt, PKC and PKCµ, p300, and HDAC4. Smartpool RNA duplexes corresponding to Src kinase, Akt kinase, PKC and PKCµ kinase, p300, HDAC4, and scrambled control 2 siRNA were purchased from Dharmacon Research Inc. (Lafayette, CO). HTSMCs (P4 or P5) were cultured onto 12-well plates. At 70 to 80% confluence, transient transfection of siRNA was carried out using Metafectene Transfection Reagent (Biontex Laboratories GmbH, Martinsried, Germany). In brief, siRNA (100 nM) was formulated with DNA-Metafectene Transfection according to the manufacturer's instructions. The transfection complex was diluted into 900 µl of DMEM/F-12 medium and added directly to the cells. The medium was replaced with complete basal essential growth medium after 3 h. Cells were analyzed at 72 h after transfection by Western blotting.

Total RNA Extraction and RT-PCR Analysis. Total RNA was isolated from HTSMCs treated with TNF- in 10-cm culture dishes with TRIzol. RNA concentration was spectrophotometrically determined at 260 nm. First-strand cDNA synthesis was performed with 2 µg of total RNA using random hexamers as primers in a final volume of 20 µl(5 µg/µl random hexamers, 1 mM dNTPs, 2 U/µl RNasin, and 10 U/µl Moloney murine leukemia virus reverse transcriptase). The reaction was carried out at 37°C for 60 min. cDNAs encoding β-actin or VCAM-1 was amplified from 3 to 5 µl of the cDNA reaction mixture using specific gene primers. Oligonucleotide primers for β-actin and VCAM-1 were as follows: β-actin, 5'-TGACGGGGTCACCCACACTGTGCCCATCTA-3' (sense) and 5'-CTAGAAGCATTTGCGGTGGACGATG-3' (antisense); and VCAM-1, 5'-GGAACCTTGCAGCTTACAGTGACAGAGCTCCC-3' (sense) and 5'-CAAGTCTACATATCACCCAAG-3' (antisense). The amplification profile includes 1 cycle of initial denaturation at 94°C for 5 min, 30 cycles of denaturation at 94°C for 1 min, primer annealing at 62°C and extension at 72°C for 1 min, and then 1 cycle of final extension at 72°C for 5 min. The expression of β-actin was used as an internal control.

Nuclear Extract. HTSMCs were seeded in a 10-cm dish. After they reached 90% confluence, the cells were starved for 24 h in serum-free DMEM/Ham's F-12 medium. After stimulation with 30 ng/ml TNF-, the cells were washed, scraped, and centrifuged to prepare cytosolic and nuclear fractions, as described previously (Wang et al., 2005). The nuclear export of HDAC4 and translocation of p65 or p50 was identified by Western blot analysis using HDAC4, p65, and p50 antibody.

Measurement of VCAM-1 Luciferase Activity. For construction of the VCAM-1-luc plasmid, human VCAM-1 promoter, a region spanning -1716 to -119 base pairs (kindly provided by Dr. W. C. Aird, Department of Molecular Medicine, Beth Israel Deaconess Medical Center, Boston, MA) was cloned into pGL3-basic vector. VCAM-1-luc plasmid was transiently transfected into HTSMCs using Genejammer Transfection Reagent (Stratagene, La Jolla, CA). In brief, plasmids (1.8 µg) and β-galactosidase (0.2 µg) were formulated with Genejammer transfection according to the manufacturer's instruction. The transfection complex was diluted into 900 µl of DMEM/F-12 medium and added directly to the cells. The medium was replaced with complete basal essential growth medium after 5 h. To assess promoter activity, cells were collected and disrupted by sonication in lysis buffer (25 mM Tris, pH 7.8, 2 mM EDTA, 1% Triton X-100, and 10% glycerol). After centrifugation, aliquots of the supernatants were tested for luciferase activity using the luciferase assay system. Firefly luciferase activities were standardized for β-galactosidase activity.

Chromatin Immunoprecipitation Assay. To detect the in vivo association of nuclear proteins with human VCAM-1 promoter, chromatin immunoprecipitation (ChIP) analysis was conducted as described previously (Nie et al., 2003) with some modifications. HTSMCs in 100-mm dishes were grown to confluence and serum-starved for 24 h. After treatment with TNF-, protein-DNA complexes were fixed using 1% formaldehyde in PBS. The fixed cells were washed and lysed in SDS-lysis buffer (1% SDS, 5 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, and 50 mM Tris-HCl, pH 8.1) and sonicated on ice until the DNA size became approximately 200 to 1000 base pairs. The samples were centrifuged, and the soluble chromatin was precleared by incubation with sheared salmon sperm DNA-protein agarose A slurry (Upstate Biotechnology) for 30 min at 4°C with rotation. After centrifugation at 800 rpm for 1 min, one portion of the precleared supernatant was used as DNA input control, and the remains were subdivided into aliquots and then incubated with a nonimmune rabbit IgG (Upstate Biotechnology), anti-p300 Ab (Santa Cruz Biotechnology), antiacetylated histone H4 Ab (Upstate Biotechnology), or anti-HDAC4 Ab (Santa Cruz Biotechnology), respectively, overnight at 4°C. The immunoprecipitated complexes of Ab-protein-DNA were collected using protein A agarose and washed successively with low-salt buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl, pH 8.1, and 150 mM NaCl), high-salt buffer (same as the low-salt buffer but with 500 mM NaCl), LiCl buffer (0.25 M LiCl, 1% Nonidet P-40, 1% deoxycholate, 1 mM EDTA, and 10 mM Tris-HCl, pH 8.1), and Tris-EDTA, pH 8.0, and then eluted with elution buffer (1% SDS and 100 mM NaHCO₃). The cross-linking of protein DNA complexes was reversed by incubation with 5 M NaCl at 65°C for 4 h, and DNA was digested with 10 µg/ml proteinase K (Sigma) for 1 h at 45°C. The DNA was then extracted with phenol-chloroform, and the purified DNA pellet was precipitated with isopropanol. The DNA pellet was resuspended in H₂O and subjected to PCR amplification with the forward primer 5'-AAATCAATTCACATGGCATA-3' and the reverse primer 5'-AAGGGTCTTGTTGCAGAGG-3', which were specifically designed from the VCAM-1 promoter region (-403 to -30). PCR products were analyzed on ethidium bromide-stained agarose gels.

CaM Kinase Activity. HTSMCs in 10-cm dishes were grown to confluence and serum-starved for 24 h. Cells were treated with 30 ng/ml TNF- for the indicated times or pretreated with 10 µM KN62 for 1 h and then incubated with TNF- for 10 min. Whole-cell lysates were immunoprecipitated with an anti-CaM kinase II Ab overnight at 4°C. The immunoprecipitated proteins were diluted with kinase buffer and analyzed by CaM Kinase II Assay Kit. In brief, 10 µl of sample was added to an Eppendorf vial (Eppendorf North America, New York, NY) containing 10 µl of assay dilution buffer II, 10 µl of CaM kinase II substrated cocktail, 10 µl of protein kinase A and PKC inhibitor cocktail, and 10 µl of [-³²P]ATP (10 mCi/ml), and then incubated for 10 min at 30°C. After incubation, 25 µl of reaction mixture was spotted on numbered P81 paper, which was rinsed sequentially using 0.75% phosphoric acid for six times and once with acetone. The P81 paper was then transferred to a scintillation vial and added scintillation cocktail. The radioactivity was counted using a scintillation counter (LS5000TA; Beckman Coulter, Fullerton, CA). The specific activity of CaM kinase II was calculated according to the directions of manufacturer.

View larger version (23K): [in this window] [in a new window]   Fig. 1. Inhibition of Src, EGFR, and PI3K/Akt prevents TNF--induced neutrophil adhesion to HTSMCs and VCAM-1 expression. HTSMCs (80% confluence in 12-well plates) were transfected with control siRNA or Src and Akt siRNA (100 nM) for 72 h as described under Materials and Methods or were pretreated with inhibitors of Src (PP1), EGFR (AG1478), and PI3K (LY294002 and wortmannin) at 10 µM for 1 h and then challenged with TNF- for 24 h (A) and 4 h (B). A, the cell lysates were subjected to 12% SDS-PAGE and transferred to nitrocellulose membrane and then blotted using an antiserum reactive with VCAM-1 or GAPDH (as an internal control) antibody as described under Materials and Methods. B, the isolated RNA samples were analyzed by RT-PCR using the primers specific for VCAM-1 and β-actin, respectively. C, TNF--stimulated Akt phosphorylation, cells were pretreated with PP1, AG1478, and LY294002 for 1 h or transfected with siRNAs (Src and Akt) and then stimulated with 30 ng/ml TNF- for 30 min. Whole-cell lysates were subjected to 12% SDS-PAGE, transferred to nitrocellulose membrane, and then blotted using an antiserum reactive with anti-phospho-Akt or GAPDH (as an internal control) antibody. Results are one representative of three independent experiments. D, neutrophils, labeled with BCECF, were added to HTSMCs incubated with TNF- for 24 h in the presence of PP1, AG1478, and LY294002, and then the adhesion was measured in the absence or presence of anti-VCAM-1 antibody (1:100 dilution) as described in Fig. 1A. Data are expressed as mean ± S.E.M. of three independent experiments. #, p < 0.01 compared with the cells exposed to TNF- alone.

Neutrophil Adhesion Assay. Peripheral blood polymorphonuclear cells (PMNs) were isolated from whole venous blood by dextran sedimentation followed by density separation over Ficoll-Hypaque and hypotonic lysis. The PMN were then resuspended in Tyrode-HEPES buffer (128 mM NaCl, 2.7 mM KCl, 0.5 mM MgCl₂, 0.36 mM NaH₂PO₄, 2 mM CaCl₂, 12 mM NaHCO₃, and 10 mM HEPES, pH 7.4) and adjusted to 1 x 10⁷ cells/ml. PMNs were used within 4 h of purification.

Neutrophils were labeled with 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) and added to an HTSMC monolayer. Nonadherent cells were removed by gentle washing with PBS, and the number of adherent cells was determined by measuring the fluorescence intensity using a CytoFluor 2300 (Millipore, Bedford, MA).

Statistical Analysis. Data were analyzed with Prism software (GraphPad Software Inc., San Diego, CA) and expressed as the mean ± S.E.M. and analyzed with a two-tailed Student's t test at a P < 0.05 level of significance.

	Results

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TNF--Enhanced Neutrophil Adhesion Was Inhibited by PP1, AG1478, LY294002, and VCAM-1 Antibody. Recently, we have reported that TNF--stimulated VCAM-1 expression in HTSMCs is dependent on mitogen-activated protein kinases and NF-B (Lee et al., 2006a). To further investigate whether an alternative pathway of Src/EGFR/PI3K/Akt was required for TNF--induced VCAM-1 expression, HTSMCs were pretreated with specific inhibitors or transfected with siRNAs. Transfection of HTSMCs with Akt or Src siRNAs for 72 h down-regulated the expression of respective proteins by 90% compared with control siRNA-transfected cells. As shown in Fig. 1, A and B, TNF--induced VCAM-1 protein and mRNA expression was significantly inhibited by pretreatment with 10 µM PP1, 10 µM AG1478, 10 µM LY294002, and 10 µM wortmannin as well as transfection with siRNA for Src and Akt. Treatment with vesicle alone had no effect on the basal level of VCAM-1 (data not shown). These findings suggested that TNF--induced VCAM-1 expression may be mediated through Src, EGFR, and PI3K/Akt cascades. Moreover, pretreatment of HTSMCs with inhibitors of Src (PP1), EGFR (AG1478), and PI3K (LY294002) for 1 h or transfection with siRNA of Src and Akt before exposure to TNF- for 30 min caused an attenuation of Akt phosphorylation (Fig. 1C). However, TNF--stimulated phosphorylation of Akt was not inhibited by calmodulin inhibitor (calmidazolium chloride) and CaMKII inhibitor (KT5926) (data not shown). To further determine the functional consequence of selective signaling inhibitors, we performed cell adhesion assays using human neutrophils adhered to HTSMCs pretreated with TNF- for 24 h. The number of adherent neutrophils dramatically increased, which was attenuated by pretreatment with PP1, AG1478, and LY294002, respectively (Fig. 1D). In particular, the adhesion activity was also blocked by an anti-VCAM-1 antibody. These results indicated that TNF--induced VCAM-1 expression was mediated through Src, EGFR, and PI3K/Akt in HTSMCs and enhanced neutrophil-HTSMC interactions.

View larger version (55K): [in this window] [in a new window]   Fig. 2. Recruitment of NF-B, p300, and Akt to VCAM-1 promoter in response to TNF-. A, neutrophils, labeled with BCECF, were added to HTSMCs pretreated with 10 µM curcumin for 1 h before incubation with TNF- for 24 h, and then the incubation was continued at 37°C for 1 h. The adhesion was measured as described in Fig. 1A. Data are expressed as mean ± S.E.M. of three independent experiments. *, p < 0.05 compared with the cells exposed to TNF- alone. B, cells were pretreated with 10 µM curcumin or MG132 for 1 h and then incubated with TNF- for 4 h. The isolated RNA samples were analyzed by RT-PCR as described in Fig. 1. C, HTSMCs (80% confluence in 12-well plates) were transfected with p300 siRNA (100 nM) for 72 h as described under Materials and Methods and then challenged with TNF- for 24 h. D, nuclear Akt and p65 coimmunoprecipitated with anti-p300 antibody. Cells were pretreated with LY294002, curcumin, and helenalin at 10 µM for 1 h before incubation with TNF- for 2 h. Equal amounts (1 mg) of nuclear extracts were immunoprecipitated (IP) with an anti-p300 Ab, separated by 10% SDS-PAGE, and immunoblotted with anti-phospho-Akt, anti-p50, or anti-p65 Ab as indicated. E and F, in ChIP assay, the sequence of the human VCAM-1 promoter region (-403/-30) was amplified by PCR primer pairs. An enrichment of the VCAM-1 promoter DNA was shown after PCR amplification of immunoprecipitates of p300- and histone H4-associated DNA from cells treated with TNF- for various times. Confluent and serum-starved HTSMCs in 10-cm dishes were pretreated with LY294002 or helenalin (HLN) for 1 h before incubation with 30 ng/ml TNF- for 2 h. The in vivo protein-DNA complexes were cross-linked by formaldehyde treatment and chromatin pellets were extracted and sonicated. The associated VCAM-1 promoter DNA was amplified by PCR as described under Materials and Methods. The input represents PCR products from chromatin pellets before immunoprecipitation.

It has been reported that p300 act as protein bridges, thereby connecting different transcriptional factors or activators via protein-protein interactions to the transcriptional machinery, such as TFIIB, RNA polymerase II complex, activator protein-1, and NF-B (Chan and La Thangue, 2001; Deng et al., 2003). In the present study, to further confirm the involvement of p300 in TNF--induced VCAM-1 expression, HTSMCs were transfected with p300 siRNA. As shown in Fig. 2C, transfection of HTSMCs with p300 siRNA down-regulated p300 protein and attenuated TNF--induced VCAM-1 expression determined by Western blotting against an anti-p300 or VCAM-1 antibody, respectively. Moreover, we presented evidence that NF-B interacted with p300 and histone H4 in vitro, immunoprecipitation of nuclear lysates with a p300 Ab, followed by immunoblot analysis using anti-p65, anti-p50, and anti-phospho-Akt Ab. As shown in Fig. 2D, p300 and histone H4 formed a complex with NF-B after TNF- stimulation, which was blocked by LY294002, helenalin (an NF-B inhibitor; Lyss et al., 1998), and curcumin. Furthermore, the roles of these transcription factors and the VCAM-1 promoter regulatory elements in TNF--induced gene transcription, and in vivo association of p300 with the VCAM-1 promoter was evaluated by a ChIP assay. To determine whether p300 could associate with the VCAM-1 promoter, PCR amplifications were conducted on an equal amount of immunoprecipitated DNA, followed by 45 cycles of PCR with the specific primer pairs encompassing position -403 to -30 regions of human VCAM-1 promoter, which contains NF-B binding sites. After TNF- treatment, an enrichment of p300- and histone H4-associated VCAM-1 promoter DNA appeared within 30 min and sustained up to 2 h, compared with the nonimmune IgG immunoprecipitated control. Association of p300 and histone H4 with the VCAM-1 promoter was blocked by pretreatment with LY294002 (Fig. 2E) or helenalin (Fig. 2F). These data indicated that NF-B, p300, and histone H4 were involved in TNF--induced VCAM-1 transcription and were regulated by a PI3K/Akt- and NF-B-dependent pathway in HTSMCs.

CaM and CaMKII Regulated TNF--Induced VCAM-1 Expression and Neutrophil Adhesion. Min et al. (2005) demonstrated that TNF-related activation-induced cytokine-induced expression of ICAM-1 and VCAM-1 in endothelial cells is regulated by PLC and calcium-dependent pathway. To determine whether PLC and Ca²⁺ mediated TNF--induced VCAM-1 expression, HTSMCs were treated with 30 ng/ml TNF- in the presence of various signaling inhibitors and revealed expression of VCAM-1 by Western blotting and semiquantitative RT-PCR. As shown in Fig. 3, A and B, pretreatment with inhibitors of PLC (U73122 [GenBank] ), Ca²⁺ chelator (BAPTA-AM), calmodulin (calmidazolium chloride), and CaMKII (KT5926) suppressed TNF--induced VCAM-1 expression at both protein and mRNA levels. In addition, KN-62 (a CaMKII inhibitor) was used to confirm the involvement of CaMKII in TNF--induced responses. As shown in Fig. 3C, pretreatment with KN62 inhibited TNF--induced VCAM-1 expression in a concentration-dependent manner.

View larger version (44K): [in this window] [in a new window]   Fig. 3. Inhibition of PLC, Ca2+/calmodulin, and CaMKII prevents TNF--induced neutrophil adhesion to HTSMCs and VCAM-1 expression. Cells were preincubated with inhibitors of phosphatidylinositol-PLC (U73122, 1 µM), Ca2+ (BAPTA-AM, 0.3 µM), CaMKII (KT5926, 1 µM), and CaM (calmidazolium chloride, 1 µM) for 1 h and then incubated with TNF- for 24 h (A) and 4 h (B). A, the cell lysates were subjected to 12% SDS-PAGE and transferred to nitrocellulose membrane to determine the level VCAM-1 protein expression as described in Fig. 1. Data are expressed as mean ± S.E.M. of three independent experiments. #, p < 0.01 compared with the cells exposed to TNF- alone. B, the isolated RNA samples were analyzed by RT-PCR using the primers specific for VCAM-1 and β-actin, respectively. C, cells were pretreated with various concentrations of KN62 (a CaMKII inhibitor) for 1 h and then incubated with TNF- for 24 h. D, TNF--stimulated CaMKII phosphorylation. Cells were pretreated with U73122, BAPTA-AM, calmidazolium chloride, and KN62 for 1 h and then stimulated with 30 ng/ml TNF- for the indicated time. Whole-cell lysates were subjected to 12% SDS-PAGE, transferred to nitrocellulose membrane, and then blotted using an antiserum reactive with anti-phospho-CaMKII Ab. Membranes were stripped and reprobed with anti-GAPDH Ab as an internal control. Results are one representative of three independent experiments. E, HTSMCs were treated with 30 ng/ml TNF- for the indicated times or pretreated with KN62 (10 µM) for 1 h and then stimulated with 30 ng/ml TNF- for 10 min. Whole-cell lysates were immunoprecipitated with an anti-CaMKII Ab overnight at 4°C. The immunoprecipitated proteins were diluted with kinase buffer and analyzed by a CaMKII assay kit (Upstate Biotechnology). Data are expressed as mean ± S.E.M. of three independent experiments. #, p < 0.05, compared with untreated basal. *, p < 0.05, compared with the cells exposed to TNF- alone for 10 min. The basal level activity of CaMKII ranged from 1.58 to 3.08 x 102 pmol/min/mg protein. F, neutrophils, labeled with BCECF, were added to HTSMCs pretreated with KT5926 and calmidazolium chloride for 1 h before incubation with TNF- for 24 h and continued incubation at 37°C for 1 h. The adhesion was measured as described under Materials and Methods. Data are expressed as mean ± S.E.M. of three independent experiments. #, p < 0.01 compared with the cells exposed to TNF- alone.

Nuclear Export of HDAC4 Was Induced by CaMKII. Recent study has demonstrated that TSA (a class I and II HDAC inhibitor) and other HDAC inhibitors block TNF--induced VCAM-1 expression and VCAM-1-dependent leukocyte adhesion under in vitro and in vivo conditions (Inoue et al., 2006). In addition, as a member of the class II HDACs, HDAC4 possesses the capability of nucleocytoplasm shuttling. The subcellular localization of HDAC4 is suggested to be regulated by CaMK. Upon phosphorylation, HDAC4 binds to a partner protein 14-3-3, which leads to an efficient nuclear export (Backs et al., 2006; Han et al., 2006). To further confirm whether TNF- induced nuclear export of HDAC4 through CaMKII, cells were stimulated by 30 ng/ml TNF- for the indicated times. The cytosolic and nuclear fractions were then used for determination of HDAC4 translocation. As shown in Fig. 4A, TNF--induced an exportation of HDAC4 out of nucleus into cytoplasm in a time-dependent manner, with a maximal effect within 60 min, sustained up to 4 h, and then declined to the basal level. Such effect can be reversed by pretreatment with calmidazolium chloride and KT5926 (Fig. 4B). In addition, the involvement of CaMK in HDAC4 translocation was assessed by an immunofluorescence staining. As shown in Fig. 4C, TNF--induced an exportation of HDAC4 out of nucleus in a time-dependent manner, consistent with the data presented in Fig. 4A. Pretreatment with KN62 (a CaMKII inhibitor) also caused an inhibition of HDAC4 exportation.

View larger version (51K): [in this window] [in a new window]   Fig. 4. HDAC4 is recruited to the VCAM-1 promoter regions and its nucleocytoplasm shuttling is regulated by CaMKII. Time-dependence of TNF--induced HDAC4 exportation from nucleus to cytosol, cells were treated with 30 ng/ml TNF- for various times (A) or in the presence of calmidazolium chloride and KT5926 at 10 µM for 1 h and then treated with TNF- for 60 min (B). Cells were harvested and centrifuged to prepare cytosolic and nuclear fractions. The resultant fractions were subjected to 10% SDS-PAGE and analyzed using an anti-HDAC4 antibody as described in Fig. 1. C, cells were stimulated with 30 ng/ml TNF- for various times or pretreated with KN62 for 1 h before incubation with TNF- for 1 h. Cells were then fixed and labeled with anti-HDAC4 Ab and an fluorescein isothiocyanate-conjugated secondary Ab. Individual cells were imaged as described under Materials and Methods. Image represents one of three individual experiments. D, an enrichment of the VCAM-1 promoter DNA was shown after PCR amplification of immunoprecipitates of HDAC4-associated DNA from cells treated with TNF- for various times. Confluent and serum-starved HTSMCs in 10-cm dishes were incubated with TNF- (30 ng/ml) for the indicated times or pretreated with KT5926 for 1 h before incubation with TNF- for 1 h. The ChIP assay was performed as described in Fig. 2. E, cells at 80% confluence in 12-well plates were transfected with control siRNA and HDAC4 siRNA (100 nM) for 72 h as described under Materials and Methods or pretreated with TSA for 1 h and then incubated with TNF- for 24 h. The cell lysates were subjected to 10% SDS-PAGE and transferred to nitrocellulose membrane to determine the level VCAM-1 protein expression as described in Fig. 1. Results represent one of three individual experiments. F, neutrophils, labeled with BCECF, were added to HTSMCs pretreated with KT5926 and TSA for 1 h before incubation with TNF- for 24 h and continued incubation at 37°C for 1 h. The adhesion was measured as described under Materials and Methods. Data are expressed as means ± S.E.M. of three independent experiments. #, p < 0.01 compared with the cells exposed to TNF- alone.

Involvement of PKC Isoforms in VCAM-1 Expression Induced by TNF-. As described in previous study, activation of PKC isoforms may be mediated through a Ca²⁺-dependent signaling. Hence, TNF--activated PKC isoforms linked to the expression of VCAM-1 was investigated in HTSMCs. Cells were incubated with 150 pg/ml TNF- in the presence of PKC inhibitors, TNF--induced VCAM-1 protein expression was revealed by Western blotting. As shown in Fig. 5A, pretreatment of HTSMCs with Gö6976 (a Ca²⁺-dependent PKC inhibitor), rottlerin (a selective of PKC- inhibitor) and Ro 31-8220 (a nonselective PKC inhibitor), significantly attenuated TNF--induced VCAM-1 expression, respectively. Moreover, it has been well established that activation of PKC could be translocated to the cell membrane. To further confirm direct PKC activation upon TNF- treatment, the cytosolic and membrane fractions were then used to determine the amount of PKC isoforms using Western blotting analysis. As shown in Fig. 5B, treatment with TNF- induced the translocation of PKC, µ, and from the cytosol to the membrane fractions, which indicated the activation of these isoforms. Long-term treatment with PMA caused a down-regulation of PKC served as negative controls. Furthermore, transfection with dominant-negative mutants of PKC and PKC significantly blocked both VCAM-1 protein and mRNA expression (Fig. 5, C and D). The involvement of these PKC isoforms in TNF--induced responses was ensured by transfection with siRNAs for PKC genes including PKC and PKCµ. Transfection of HTSMCs with PKC and PKCµ siRNAs for 72 h down-regulated total proteins expression by 80% compared with control siRNA-transfected cells and thus attenuated the TNF--induced VCAM-1 expression (Fig. 5E). These results indicated that TNF--induced VCAM-1 expression was mediated through activation of PKC, µ, and isoforms in HTSMCs.

View larger version (43K): [in this window] [in a new window]   Fig. 5. Involvement of PKC isoforms in TNF--induced VCAM-1 expression. A, HTSMCs were pretreated with Gö6976, rottlerin, and Ro 31-8220 at 10 µM for 1 h and then incubated with 150 pg/ml TNF- for 24 h. The cell lysates were subjected to 10% SDS-PAGE and transferred to nitrocellulose membrane to determine the level of VCAM-1 expression as described in Fig. 1. Data are expressed as mean ± S.E.M. of three independent experiments. #, p < 0.01 compared with the cells exposed to TNF- alone. B, time-dependence of TNF--stimulated translocation of PKC isoforms from cytosol to cell membrane. Cells were treated with 30 ng/ml TNF- for various times or with PMA for 24 h. Cells were harvested and centrifuged to prepare cytosolic and membrane fractions. The resultant fractions were subjected to 10% SDS-PAGE and analyzed with anti-PKC isoforms antibodies as described in Fig. 1. To confirm the involvement of PKC isoforms in VCAM-1 expression, cells were transfected with dominant-negative mutants of PKC and PKC and then incubated with TNF- for 24 h (C) and 4 h (D). C, the cell lysates were subjected to 12% SDS-PAGE and transferred to nitrocellulose membrane to determine the level of VCAM-1 expression as described in Fig. 1. Results represent one of three individual experiments. D, the isolated RNA samples were analyzed by RT-PCR using the primers specific for VCAM-1 and β-actin, respectively. E, HTSMCs (80% confluence in 12-well plates) were transfected with siRNA for PKC and PKCµ (100 nM) for 72 h as described under Materials and Methods and then challenged with 150 pg/ml TNF- for 24 h. The cell lysates were subjected to 10% SDS-PAGE and transferred to nitrocellulose membrane to determine the level of VCAM-1, PKC, and PKCµ expression as described in Fig. 1. Results represent one of three individual experiments.

TNF--Induced VCAM-1 Promoter Activity. This regulation of VCAM-1 gene transcription through Src/EGFR/PI3K/Akt and PLC/Ca²⁺/calmodulin/CaMKII pathways induced by TNF- was further confirmed by gene luciferase activity assay. HTSMCs were transfected with VCAM-1 luciferase reporter gene and then stimulated with TNF- (30 ng/ml) for the indicated time. Data in Fig. 6A showed that TNF--stimulated VCAM-1 luciferase activity reached a maximal response within 60 min and sustained up to 4 h. Moreover, TNF--induced VCAM-1 promoter activation was inhibited by selective inhibitors, including AG1478, LY294002, U73122 [GenBank] , BAPTA-AM, calmidazolium chloride, and KT5926 for EGFR, PI3K, PLC, Ca²⁺, calmodulin, and CaMKII, respectively (Fig. 6B). These results confirmed that TNF--stimulated VCAM-1 luciferase gene activity involved at a transcription level mediated through activation of Src/EGFR/PI3K/Akt and PLC/Ca²⁺/calmodulin/CaMKII pathways in HTSMCs.

View larger version (24K): [in this window] [in a new window]   Fig. 6. Inhibition of Src/EDGFR/PI3K/Akt and PLC/Ca2+/camodulin/CaMKII pathways attenuates TNF--induced VCAM-1 promoter activity. Cells transiently transfected with VCAM-1 reporter gene were treated with TNF- (30 ng/ml) for various times (A) and pretreated with PP1, AG1478, LY294002, U73122, BAPTA-AM, calmidazolium chloride, KT5926, and GM6001 for 1 h before incubation with TNF- for 4 h (B). Luciferase activity was assayed as described under Materials and Methods. Data are expressed as mean ± S.E.M. of three independent experiments. *, p < 0.05; #, p < 0.01 compared with the cells exposed to vesicle (A) or TNF- alone (B).

	Discussion

Top Abstract Materials and Methods Results Discussion References

 
Many of the common airway diseases, including asthma and chronic obstructive pulmonary disease, involve inflammation, with the coordinate expression of multiple inflammatory genes in the airway tissues. These inflammatory genes encode for the expression of cytokines, chemokines, enzymes, and adhesion molecules (Barnes et al., 2005; Yang et al., 2005). Chromatin remodeling initiated by reversible acetylation of core histones by HAT and HDACs is considered to be a key element in the dynamic regulation of all gene expressions (Roth et al., 2001). Alterations in balance between histone acetylation and deacetylation could modulate cellular function, including cell growth, differentiation, cell death, cell-cell and cell-matrix interactions, and inflammatory responses. In fact, there is growing evidence in the physiological and pathological relevance of HAT and HDAC in the respiratory system: HDAC activity is decreased in patients with asthma, whereas HAT activity is increased in biopsy specimens (Ito et al., 2005). Thus, HDAC and HAT can be therapeutic targets for airway inflammatory diseases.

The inflammatory responses may be mediated by complex interactions between both circulating PMNs and endothelium. Others and our recent studies have been well documented that up-regulation of adhesive molecules on the cell surface of airway or lung cells plays a critical role in the inflammation action (Huang et al., 2003; Lee et al., 2003; Wang et al., 2005; Lee et al., 2006a). In the present study, we have demonstrated a novel regulatory action of TNF- and its underlying signaling mechanisms between HAT and HDAC associated with VCAM-1 gene expression in HTSMCs. At first, we showed that TNF- enhanced HTSMC-neutrophil interaction, correlated with expression of the VCAM-1 in HTSMCs. In addition, we also demonstrated a critical role of the inflammatory transcription factor NF-B and p300 (a histone acetyltransferase) in the TNF--induced transcription of VCAM-1. Furthermore, our results clearly demonstrated that Akt played an important role in mediating the TNF--dependent production of VCAM-1 in HTSMCs through the downstream activation of p300 but not NF-B activation. Induction of VCAM-1 and phosphorylation of Akt in response to TNF- were significantly attenuated in parallel by the inhibition of phosphorylation of Src, EGFR, and PI3K by their respective inhibitors of PP1, AG1478, and LY294002. Moreover, we used transfection of siRNAs for Src and Akt specifically down-regulated the expression of either Src or Akt protein. Knockdown expression of these proteins also attenuated TNF--induced Akt activation and VCAM-1 expression in HTSMCs (Fig. 1). These results suggested that TNF- stimulated p300 associated with VCAM-1 promoter (containing NF-B binding sites) via the Src/EGFR/PI3K/Akt-dependent signaling cascades triggered upon receptor engagement.

Under normal conditions, HAT transfers an acetyl group to the core histones of a lysine residue, and the acetylation level of chromatin has been established to be a key mechanism in derepressing gene transcription. Conversely, gene repression is mediated via HDACs. Inoue et al. (2006) have demonstrated that TNF--induced VCAM-1 expression and VCAM-1-dependent endothelial-leukocyte adhesion were inhibited by pretreatment with TSA (a class I and II HDAC inhibitor) and transfection with siRNAs for HDAC3. In contrast, recent study reveals that TSA and NaBu (HDAC inhibitors) enhance the IL-5 promoter activity. Induction of IL-5 can be repressed by transfection with wild-type plasmid of the HDACs (Han et al., 2006). Moreover, HDAC, which removes the acetyl groups from hyperacetylated histones, counteracts the effects of HAT, and returns histone to its basal state, finally leads to the suppression of gene transcription. Moreover, aberrant regulation of HDACs plays an important role in human carcinogenesis. It has been shown that TSA inhibits HDAC and has potent antitumor activity in many human cancers (Huang, 2006; Lin et al., 2006). In contrast, TSA increases histone H3 acetylation of VCAM-1 and ICAM-1 promoters in lung endothelial cells (Rose et al., 2005). However, in our study, we provided the evidence of HDAC inhibitors to exert as a negative regulator in VCAM-1 expression. Pretreatment with TSA or transfection with siRNA-mediated knockdown of HDAC4 in HTSMCs and resulted in a significant reduction in TNF--enhanced neutrophil adhesion and VCAM-1 expression (Fig. 4, E and F). These differences may be due to cell-specific or different experimental conditions.

View larger version (25K): [in this window] [in a new window]   Fig. 7. Schematic pathways illustrated that TNF--mediated VCAM-1 induction was indeed governed by transcript-specific signaling pathway involving Src/EGFR/PI3K and PLC/Ca2+/CaM. Phosphorylation of Akt and CaM kinase II by these two pathways may eventually lead to enhancing the activity of HAT and HDAC. Finally, the elevated VCAM-1 expression on HTSMCs is involved in causing PMN migration and airway inflammatory responses.

On the other hand, it is known that class II HDAC is activated by nucleocytoplasmic shuttling through CaMK-dependent phosphorylation (Huang and Chen, 2005; Backs et al., 2006; Sato et al., 2006; Little et al., 2007). To further verify the nuclear exportation of HDAC4 induced by TNF-, the ChIP assay and cytosolic/nuclear fractions were used for this purpose. Our data revealed that TNF- stimulation caused HDAC4 exported from the nucleus to the cytoplasm to be reversed by adding calmidazolium chloride (an inhibitor of calmodulin) and KT5926 (an inhibitor of CaMKII). Taken together, these data strongly suggest that CaMKII-dependent HDAC4 activation may play important roles in transcriptional regulation of the VCAM-1 gene, consistent with HDAC4 as a specific downstream substrate of CaMKIIBin cardiac cells (Little et al., 2007). These results suggest that TNF--induced VCAM-1 expression was, at least in part, mediated through activation of CaMKII. However, a role of CaMKII isoforms in TNF--induced responses needs to be investigated in HTSMCs.

It is worth noting that the inhibitors of Src, EGFR, PI3K, and phosphatidylinositol-PLC seemed to be able to completely inhibit TNF--induced VCAM-1 expression and neutrophil adhesion when used alone, suggesting that these pathways were interconnected. These protein kinases may converge at a step downstream to activate transcription factors such as NF-B and p300 binding to VCAM-1 promoter. This interaction between p300 and NF-B may be required for the induction of VCAM-1 by TNF-. Therefore, inhibition of one of these components was able to interrupt the signaling transduction associated with VCAM-1 expression. However, the complicated regulatory mechanisms underlying TNF--induced VCAM-1 expression need to be investigated in the future.

Next, Min et al. (2005) have shown that PLC, PI3K, and PKC are crucial downstream signals of TRAFs, leading to the expression of adhesion molecules. Our previous studies have also reported that activation of PKC is required for the induction of cyclooxygenase-2 and cytosolic phospholipase A₂ in rat brain astrocytes (Hsieh et al., 2005, 2007). Furthermore, PKC-stimulated phosphorylation of c-Src may eventually result in NF-B activation and ICAM-1 expression (Huang et al., 2003). Herein, three PKC isoforms, namely PKC-, PKC-µ, and PKC-, have been activated in response to TNF- in HTSMCs. Moreover, TNF--induced expression of VCAM-1 was significantly attenuated by pretreatment with PKC inhibitors or transfection with dominant-negative mutants of PKC and PKCµ.

In summary, the overall pathway by which TNF- induces VCAM-1 expression is illustrated in Fig. 7. TNF- is an airway inflammatory mediator that increases the expression of inflammatory molecule VCAM-1 via sequential activation of PKCs, Src/EGFR/PI3K/Akt/p300, and PLC/Ca²⁺/calmodulin/CaMKII/HDAC4 signaling pathways in HTSMCs. Our findings, together with previous observations, suggest that elevated VCAM-1 expression is involved in causing PMN migration and inflammation responses.

	Acknowledgements

 
We greatly appreciate Dr. P. Parker (Cancer Research UK, London, UK) for providing dominant-negative plasmids of PKC and PKC for this study.

	Footnotes

 
This work was supported by grants from National Science Council (NSC96-2320-B-182-003 and NSC96-2314-B-182-011, and NSC95-2320-B-182-047-MY3) and Chang Gung Medical Research Foundation (CMRPD-32043, CM-RPG-350651, and CMRPG-33028).

ABBREVIATIONS: TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule; CBP, cAMP response element-binding protein binding protein; ChIP, chromatin immunoprecipitation; DMEM, Dulbecco's modified Eagle's medium; EGFR, epidermal growth factor receptor; HAT, histone acetyltransferase; HTSMC, human tracheal smooth muscle cell; PI3K, phosphatidylinositol 3-kinase; CaMK, calmodulin-dependent kinase; NF-B, nuclear factor B; HDAC, histone deacetylase; PCR, polymerase chain reaction; RT-PCR, reverse-transcriptase polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PMA, phorbol 12-myristate 13-acetate; PMNs, polymorphonuclear cells; PKC, protein kinase C; PBS, phosphate-buffered saline; PAGE, polyacrylamide gel electrophoresis; BSA, bovine serum albumin; siRNA, short interfering RNA; IL, interleukin; ICAM, intercellular adhesion molecule; Sp1, Simian virus 40 promoter factor 1; Ab, antibody; ECL, enhanced chemiluminescence; BAPTA-AM, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester; TSMC, tracheal smooth muscle cell; TSA, trichostatin A; BCECF, 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; PLC, phospholipase C; PP1, 4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]-pyrimidine; AG1478, 4-(3'-chloroanilino)-6,7-dimethoxy-quinazoline; LY294002, 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride; U73122 [GenBank] , 1-[6-[[17β-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione; Gö6976, 12-(2-cyanoethyl)-6,7,12,13-tetrahydro-13-methyl-5-oxo-5H-indolo(2,3-a)pyrrolo(3,4-c)-carbazole; Ro 31-8220, 3-1-[3-(amidinothio)propyl-1H-indol-3-yl]-3-(1-methyl-1H-indol-3-yl) maleimide (bisindolylmaleimide IX); KT5926, (8R^*,9S^*,11S^*)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-14-n-propoxy-2,3,9, 10-tetrahydro-8,11-epoxy, 1H,8H,11H-2,7b,11a-triazadibenzo[a,g]cycloocta[cde]trinden-1-one; KN62, 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine; MG132, N-benzoyloxycarbonyl (Z)-Leu-Leu-leucinal.

Address correspondence to: Dr. Chuen-Mao Yang, Department of Physiology and Pharmacology, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-San, Tao-Yuan, Taiwan. E-mail: chuenmao{at}mail.cgu.edu.tw

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