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Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina (S.S., R.R., J.A.G.); and Department of Pathology, Northwestern University, the Feinberg School of Medicine, Chicago, Illinois (J.K.R.)
Received for publication May 19, 2008.
Accepted for publication June 13, 2008.
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Abstract |
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(HNF4)-interacting protein. CYP2C9 is an important enzyme that metabolizes both commonly used therapeutic drugs and important endogenous compounds. We have shown previously that constitutive androstane receptor (CAR) (a xenobiotic-sensing receptor) up-regulates the CYP2C9 promoter through binding to a distal site, whereas HNF4 transcriptionally up-regulates CYP2C9 via proximal sites. We demonstrate ligand-enhanced synergistic cross-talk between CAR and HNF4. We identify NCOA6 as crucial to the underlying mechanism of this cross-talk. NCOA6 was identified as an HNF4-interacting protein in this study using a yeast two-hybrid screen and GST pull-down assays. Furthermore, we identified NCOA6, CAR, and other coactivators as part of a mega complex of cofactors associated with HNF4 in HepG2 cells. Although the interaction of NCOA6 with CAR is specifically through the first LXXLL motif of NCOA6, both LXXLL motifs are involved in its interaction with HNF4. Silencing of NCOA6 abrogated the synergistic activation of the CYP2C9 promoter and the synergistic induction of the CYP2C9 gene by CAR-HNF4. Chromatin immunoprecipitation analysis revealed that NCOA6 can pull down both the proximal HNF4 and distal CAR binding sites of the CYP2C9 promoter and provides the basis for the recruitment of other cofactors. We conclude that the coactivator NCOA6 mediates the mechanism of the synergistic activation of the CYP2C9 gene by CAR and HNF4.
). Hepatic expression of CYP2C9 exhibits considerable interindividual variability in humans. Some of this interindividual variability is due to the up-regulation of CYP2C9 levels by prior exposure to drugs and xenobiotics such as rifampicin, hyperforin, phenobarbital, and paclitaxel (Taxol) (Raucy et al., 2002; Madan et al., 2003; Komoroski et al., 2004). Studies in primary hepatocytes and clinical studies in vivo in humans have confirmed that CYP2C9 levels and the clearances of CYP2C9 substrates are increased after the administration of drugs (Williamson et al., 1998; Henderson et al., 2002).
Recent studies have shown that the constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) both bind to responsive elements (REs) in the CYP2C9 promoter and are responsible for the transcriptional up-regulation of CYP2C9 by various drugs (Ferguson et al., 2002; Gerbal-Chaloin et al., 2002; Chen et al., 2004). There is considerable overlap between the two receptors for similar sets of responsive elements in the promoters of various genes (Xie et al., 2000; Goodwin et al., 2001; Smirlis et al., 2001). Each of these receptors is preferentially activated by a wide range of structurally unrelated compounds (Sueyoshi et al., 1999; Moore et al., 2000). Human CAR is activated preferentially by compounds such as phenobarbital, chlorpromazine, clotrimazole, methoxychlor, and CITCO (Timsit and Negishi, 2007), whereas PXR is activated preferentially by ligands such as rifampicin (rifampin) (Timsit and Negishi, 2007), paclitaxel, and hyperforin (Xie et al., 2000). The critical feature for activation of CAR by xenobiotics is its translocation from the cytoplasm to the nucleus, where it heterodimerizes with retinoid X receptor, which facilitates its binding to CAR REs within the DNA of various promoters (Honkakoski et al., 1998; Kawamoto et al., 1999; Sueyoshi et al., 1999; Suino et al., 2004; Xu et al., 2004; Moore, 2005). Activation of CAR elicits a pleiotropic response regulating diverse pathways including various cytochrome P450 enzymes, liver growth, and liver tumor promotion by phenobarbital (Yamamoto et al., 2004).
Regulation of various promoters by CAR is substantially influenced by other nuclear receptors and transcription factors. We have shown that the hepatic enriched transcriptional factor hepatic nuclear factor 4 (HNF4) transcriptionally up-regulates the CYP2C9 promoter after binding to at least two proximal direct repeats; furthermore, HNF4 and CAR synergistically activate the CYP2C9 promoter in HepG2 cells, and mutation of the HNF4 sites reduces or abolishes CAR-mediated induction of CYP2C9 (Chen et al., 2005). This suggests a potential cross-talk between a CAR site at -1839 bp and one of the two proximal HNF4 binding sites in the CYP2C9 promoter. The present study addresses the mechanism of this cross-talk.
Cofactors interact with nuclear receptors in the presence of ligands to bring about successful completion of gene transcription (Rosenfeld and Glass, 2001; McKenna and O'Malley, 2002). These cofactors have been found to be associated as complexes; several such complexes have been purified: DRIP (Rachez et al., 1999), TRAP (Fondell et al., 1996), and PRIC (Surapureddi et al., 2002). Cofactors identified in such complexes include activators of the p160 family (Rosenfeld and Glass, 2001; McKenna and O'Malley, 2002), CREB binding protein/p300 (Chrivia et al., 1993; Eckner et al., 1994), and mediator proteins such as PBP (Zhu et al., 1997), PRIP (Zhu et al., 2000), and PGC-1 (Puigserver et al., 1999). These cofactors all contain one or more conserved LXXLL motifs, which have been found to be necessary for ligand-dependent interaction with the AF-2 domain (Heery et al., 1997). PGC-1 has been studied extensively, also as a coactivator of HNF4 (Lin et al., 2005). In the present study, we identify NCOA6 as a new interacting partner of HNF4. NCOA6 is reported to belong to a novel steady-state complex called ASCOM (ASC-2/PRIP complex) that contains a subset of trithorax group of proteins (Goo et al., 2003). The current model of NR-mediated transcription proposes that subsets of coactivator complexes contribute sequentially to the multiple subreactions of the transcription process (McKenna et al., 1999; Lonard and O'Malley, 2006).
The present study first identifies the cofactor NCOA6 as a new HNF4-interacting partner using yeast two-hybrid screens and protein-protein interaction studies. NCOA6, HNF4, and CAR were identified as part of a mega complex in HepG2 cells. Chromatin immunoprecipitation (ChIP) assays show that antibody to NCOA6 precipitated both the CAR and HNF4 binding sites of the CYP2C9 promoter. Finally, silencing NCOA6 abolished the synergistic activation of the CYP2C9 gene by CAR and HNF4 and reduced the recruitment of coactivators and methyltransferases to the HNF4 sites. These results strongly indicate that NCOA6 is crucial for the formation of a bridge between the CAR and HNF4 receptor sites in the CYP2C9 promoter and for the cross-talk between these two receptors.
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Materials and Methods |
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). Full-length HNF4 or partial domains were used as bait. Human liver was used to prepare "prey" libraries. Baits were mated with prey, and selection was based on the dropout media (-Trp, -Leu, -His, -Ade) plates. Interactions between bait and prey molecules were identified using His- and Ade-selections. Plasmids isolated were retransformed into yeast, and interactions were confirmed by liquid β-galactosidase assays. The identities of the prey were determined by DNA sequencing.
Plasmids and Adenovirus-Mediated Expression and RNA Interference. CAR, HNF4, NCOA6, PBP, and PGC-1 were cloned in pcDNA3.1 by PCR amplification. GST-CAR and GST-HNF4 were cloned in pGEX-4T1. NCOA6 domains, NCOA6 I (1-353 aa; NC-I), NCOA6 II (338-673 aa; NC-II), NCOA6 III (648-998 aa containing the first LXXLL motif at 851 aa; NC-III), NCOA6 IV (986-1327 aa; NC-IV), NCOA6 V (1292-1641 aa coding for the second LXXLL motif at 1491 aa; NC-V), NCOA6 VI (1625-2065 aa; NC-VI), and NCOA6 VII (648-1641 aa; NC-VII) were cloned in pGEX-4T1 and pcDNA3.1. CYP2C9-1.9Kb/pGL3 construct, described previously (Chen et al., 2005), was used for the transient transfection assay of CYP2C9 promoter expression. Adenovirus expressing full-length CAR and HNF4 were made with AdEasy XL Adenoviral Vector system (Stratagene, La Jolla, CA). Virus particles were purified on continuous cesium chloride, dialyzed, and stored in Tris-buffered sucrose. Small interfering RNA (siRNA) targets for NCOA6 were identified using Genscript's target finder, construct builder, and sequence scrambler for construction of negative control siRNA. The following siRNAs were designed to silence NCOA6 mRNA coding sequence: NC-I, @146 bp: 5'-TTGTGGCCTTCAAAGGAAATA-3'; NC-II, @500 bp: 5'-TGGCAAGTGGTCCAGGAATAA-3'; NC-III, @2241 bp: 5'-CTCCGAACATGCAAGGAAATA-3'; NC-IV, @2451 bp: 5'-GATGCCTGATGTTAGCATTCA-3'; and NC-V, @3262 bp: 5'-GTGCCACCATCACCTGATAAA-3'. Using the construct builder, double-stranded short hairpin RNA oligonucleotides were designed for pRNAT-H1.1/Adeno (SD-1219) with H1 promoter and cGFP as the marker. NC-III siRNA target sequence was used to design the scrambled siRNA 5'-CACTGAAGTATACCAAGAGCA-3'. Adenoviruses expressing each short hairpin RNA were prepared and purified. HepG2 cells were routinely infected with 2.5 x 109 virus particles (VP).
Cell Culture, Transient Transfections, and Ligands. HepG2 cells were maintained in the Eagle's minimal essential medium supplemented with 10% fetal bovine serum and penicillin-streptomycin at 37°C under 5% CO2. All transient transfections were carried out as described in Lipofectamine 2000 protocol (Invitrogen, Carlsbad, CA). In brief, 0.2 µg of CYP2C9 luciferase construct, 0.1 µg of each receptor construct, and 0.1 µg of coactivator construct (2:1:1) with 0.02 µg of pRL-TK vector as internal control, and pcDNA 3.1 as the empty vector to make the total amount of DNA transfected to 0.8 µg were combined in 50 µl of OPTI-MEM, mixed with transfection reagent as suggested, and overlaid on 80 to 90% confluent HepG2 cells in serum-containing media. Twenty-four hours later, medium was replaced, and ligands were added at the appropriate concentrations (0.1% of dimethyl sulfoxide and 0.1 µM CITCO). Ligands were incubated with the HepG2 cells for 24 h and assayed for promoter activity using a dual luciferase assay kit (Promega, Madison, WI). Firefly luciferase readings were normalized with Renilla reniformis readings to calculate promoter activity.
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Expression HepG2 Nuclear Extracts, Isolation of Interacting Proteins, and Immunoblotting. Ten plates (15 cm) of 90% confluent HepG2 cells were infected with 2.5 x 1011 VP for 48 h with either AdCAR or AdHNF4. The cells were harvested after a quick wash in ice-cold phosphate-buffered saline; nuclear extracts were prepared according Dignam et al.'s method (Dignam et al., 1983). GST and GST-HNF4 fusion proteins immobilized on GSH-Sepharose beads were incubated with 5 to 10 mg of AdCAR-overexpressing HepG2 nuclear extracts (AdCAR-NEs) overnight. The beads were extensively (100 volumes) washed after incubation with TEGN buffer (20 mM Tris, pH 8.0, 0.2 mM EDTA, 10% glycerol, and 0.1% Nonidet P-40) containing 180 mM NaCl and once with TEGN buffer containing no salt. The bound proteins were eluted in SDS-PAGE sample buffer and heat-denatured. The proteins were separated ona4to20% gradient gels and silver stained for visualization. GST and GST-HNF4 bound proteins after separation on 4 to 20% gradient gels were transferred onto nitrocellulose membrane, blocked with 5% milk in Tris-buffered saline/Tween 20, and immunoblotted with antibodies for CAR (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) and reconfirmed with a monoclonal CAR antibody from R&D Systems (Minneapolis, MN), HNF4, CBP, PIMT, PGC-1 (all from Santa Cruz Biotechnology), and NCOA6 (Bethyl Laboratories, Montgomery, TX).
The Protein Microcharacterization Facility at NIEHS provided protein sequencing-mass spectrometry. Protein bands of GST and GST-HNF4 lanes were sliced in 2-mm thickness and tryptic-digested. Coomassie blue-stained SDS-PAGE gels were cut, and proteins were digested with trypsin essentially as described in Choi et al. (2007). Resulting peptide digests were then analyzed by nano-LC electrospray ionization-mass spectrometry and MS/MS on and Agilent XCT Ultra ion trap mass spectrometer (Agilent Technologies, Palo Alto, CA), and data were processed and searched against the National Center for Biotechnology Information nonredundant database as described previously (Choi et al., 2007).
Expression and Purification of Recombinant Proteins, GST Pull-Down Assay. Full-length recombinant proteins of CAR and HNF4 were expressed as GST fusion proteins in Escherichia coli BL21 (DE3). GST pull-down assays were performed by incubating 5 µl of [35S]methionine-labeled proteins in a 500 µl of NETN buffer containing 1 mg/ml fatty acid-free bovine serum albumin in the presence and absence of respective ligands. The bound proteins were washed three times with NETN buffer and were heat-denatured in SDS sample buffer. These proteins were separated on 4 to 20% gradient gels, and after fixing and amplifying the signals, the gels were dried and autoradiographed.
qPCR-Total RNA was extracted using RNeasy MiniPrep system (QIAGEN, Valencia, CA). Reverse transcription PCR analysis was performed in two steps by initial reaction with Superscript II (Invitrogen, Carlsbad, CA) reverse transcriptase. PCR with Taqman Universal PCR Master Mix (Applied Biosystems, Foster City, CA) was then performed with gene-specific primers using relative quantification methods (2-
CT) and measured on an Applied Biosystems Geneamp PCR System 9700 using Taqman probes for CYP2C9, NCOA6, PGC-1, PBP, SRC-1, GRIP-1, and HNF4 with TBP as the internal control.
ChIP Analysis. Five plates (15 cm) of 90% confluent HepG2 cells were infected with 2.5 x 1011 VP for 48 h each with adenovirus-expressing lacZ, CAR, HNF4, CAR-HNF4, CAR-HNF4 with siRNA for NCOA6 (NC-III), and CAR-HNF4 with siRNA for NCOA6 (NC-IV) individually. After 48 h, the cells were cross-linked with 1% formaldehyde directly in the media for 10 min, and the chromatins were prepared (Qi et al., 2003). Chromatin extracts were precleared by incubating with rabbit serum for 3 h, and the IgG-bound proteins were pulled down by incubating with preswollen Protein A beads. The supernatant was stored or used for immunoprecipitations. All of the chromatins used in the immunoprecipitations were checked to contain equal amounts of the target gene by PCR amplification and adjusted to 100 µl, which was further diluted to 500 µl to be used in the immunoprecipitation with 2 µg of each antibody. CAR, CBP, NCOA6, and PIMT immunoprecipitates were washed twice with buffer C and four times with buffer D. HNF4 antibody precipitates were washed three times with buffer C and six times with buffer D (buffers described previously) (Qi et al., 2003). The DNA binding proteins bound cognate cis-acting elements, and DNA fragments from the chromatin extracts (inputs) were purified and used as control for PCR reactions. The primers used for amplification of the human CYP2C9 promoter are 5'-TAAAGACAGCAACCGAGC-3' and 5'-TACAATGATTCAGGATTTCG-3' spanning for the CAR-response element and 5'-ATATACAAGGCATAGAATATGGCC-3' and 5'-GACCAATCACCTAGGTCCAC-3' spanning the HNF4-response elements. Negative control primers are 5'-ATGGTTGCCACTGGGGATCT-3' and 5'-TGCCAAAGCCTAGGGGAAGA-3'.
Statistical Comparisons. Results were analyzed by two-way or one-way analysis of variance, and pair-wise comparisons were made using Bonferroni t test.
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Results |
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TopAbstractMaterials and MethodsResultsDiscussionReferences |
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as the bait. Two of 11 HNF4 baits (93-352 and 165-355 aa) identified several strong interacting proteins that could pass two rounds of screening. One of the prey proteins identified by both of the bait molecules was NCOA6 (1993-2726 and 2322-2724 bp) coding for the first LXXLL motif. This is the first time NCOA6 has been identified as an HNF4-interacting protein. Other known coactivators of HNF4 identified by the screen include PGC-1. The complete coding sequence of human NCOA6 protein was assembled in pcDNA3.1(+) using IMAGE and EST clones (Invitrogen).
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. Nuclei were isolated from HepG2 cells infected with AdCAR for 48 h to ensure the translocation of CAR and its binding proteins to induce the transcriptional activity of the target gene of interest, CYP2C9. To maximize the capture of proteins, we used full-length HNF4. HepG2 nuclear extracts enriched with CAR were incubated with immobilized GST and GST-HNF4. After extensively washing, the bound proteins were denatured and subjected to SDS-PAGE. Very few nuclear proteins were bound to GST alone (Fig. 1a, lane 2). In contrast, more than 25 nuclear proteins with electrophoretic motilities greater than 40 kDa were detected that bound to GST-HNF4 (lane 4). An equivalent amount of GST-HNF4 purified from bacteria is shown as a control (lane 3). Protein sequencing data identified a polypeptide band with a molecular mass of 
40 kDa as the constitutive androstane receptor (Fig. 2). CAR (genInfo identifier number 83921568) was positively identified in the GST-HNF4 bound proteins and was not observed in the GST-only bound proteins. CAR was identified with a SpectrumMill distinct summed MS/MS search score of 94.23 with 22% sequence coverage (Fig. 2a). Specifically, six different tryptic peptides (Fig. 2b) that are unique to CAR were observed via mass spectrometry and MS/MS. Each of the six peptides was unambiguously identified by extensive b- and y-series ions in MS/MS experiments, as seen from the representative MS/MS spectra shown in Fig. 2 (c and d).
Although current identification of additional large proteins by protein microsequencing is in progress, we used immunoblotting to identify some of the expected cofactors in the GST-HNF4 binding complex. We also detected CAR by immunoblotting in both the input and in the proteins bound to GST-HNF4 but not in proteins eluting from GST confirming the MS/MS results (Fig. 1b). Because we identify CAR and HNF4 in nuclear complexes in HepG2 cells but were unable to show evidence of a direct interaction between CAR and HNF4 using GST-HNF4 and radiolabeled CAR or GST-CAR and radiolabeled HNF4 (data not shown), we hypothesized that cofactors might be responsible for bringing these two nuclear receptors together as a nuclear mega-complex. We have identified several cofactors, including NCOA6, CBP, PGC-1, and PIMT, by immunoblotting the Ad-CAR-expressing nuclear extracts (inputs) and in proteins bound to GST-HNF4 but not in proteins retained by GST alone, suggesting that the HNF4-CAR complex represents a functional transcriptional complex (Fig. 1, a and b). The identification of NCOA6 and PGC-1 as HNF4-interacting proteins by immunoblotting was consistent with the results of our two-hybrid screen. PIMT is also a known NCOA6-interacting protein with a methyltransferase domain.
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. To identify NCOA6 interactions, we first used a GST fusion protein of a truncated NCOA6 (648-900 aa; coding for the first LXXLL motif). GST-NCOA6 was used for binding assays with [35S]methionine-labeled HNF4 and CAR. Both of the nuclear receptors bound to GST-NCOA6 strongly in the absence and presence of CITCO, which is known to be a high-affinity ligand for human CAR (Maglich et al., 2003). To verify the reciprocal relationships, GST-CAR and GST-HNF4 were incubated with in vitro-translated full-length NCOA6, and the known coactivators PBP and PGC-1 were used as a positive control. GST-CAR and GST-HNF4 fusion proteins retained the radiolabeled NCOA6, PBP, and PGC-1 in the presence and absence of 100 nM CITCO; GST alone did not retain NCOA6 (Fig. 3a). The CAR ligand CITCO augmented the retention of NCOA6 and PBP by GST-CAR. As expected, GST-CAR and GST-HNF4 fusion proteins also retained other in vitro-translated known coactivators such as SRC-1 and GRIP (data not shown). To define the interacting domain(s) of NCOA6, GST-NCOA6 domains were allowed to interact with in vitro [35S]methionine-translated CAR or HNF4. NCOA6 domains expressed as GST fusion proteins are shown schematically in Fig. 3b. GST-NCOA6-III domain (648-998 aa; the first LXXLL at 851 aa) interacted significantly with CAR (Fig. 3c) in a ligand-independent manner. The other NCOA6 domains did not interact appreciably with CAR, suggesting that only the first LXXLL motif was involved in the interaction. The first LXXLL motif of NCOA6 has been shown previously to interact with other nuclear receptors such as peroxisome proliferator-activated receptor ( and 
), thyroid hormone receptor alpha, and estrogen receptor- (Zhu et al., 2000). The NCOA6-CAR interaction differs from PBP-CAR interactions in that PBP requires both LXXLL motifs to bind with CAR (Jia et al., 2005). The interacting domains between NCOA6 and HNF4 were similarly mapped with [35S]methionine-HNF4 (Fig. 3d); the GST-NCOA6-III domain strongly retained the radiolabeled HNF4, suggesting a robust interaction. We were surprised to find that there was also a moderate interaction between the GST-NCOA6-V domain (containing the second LXXLL motif) and HNF4, suggesting a new role for this LXXLL motif. The second LXXLL motif of NCOA6 was also recently shown to be involved in the interaction with LXR (another liver-specific receptor) in regulating lipogenesis and cholesterol/bile acid homeostasis in the liver and interaction with estrogen receptor- (Li et al., 2007a).
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Synergistically Activate the CYP2C9 Promoter in a Ligand-Enhanced Manner, and NCOA6 Modestly Augments This Effect. CAR transactivates the CYP2C9 promoter, and the CAR ligand CITCO enhances this effect (p < 0.01). The activation is 5-fold in the absence of ligand and 11-fold in the presence of 100 nM CITCO (Fig. 4a). This concentration of ligand was used because initial experiments verified that it is specific for CAR, whereas higher doses are not specific because they activate another receptor, PXR. Coexpression of NCOA6 did not significantly enhance CAR-mediated promoter activation, whereas PGC-1, a known CAR coactivator, significantly enhanced activation (p < 0.001). HNF4 transactivated CYP2C9 promoter activity (7-fold) (p < 0.05) (Fig. 4b), and coexpression of NCOA6 with HNF4 modestly enhanced this activation to 9-fold (p < 0.001), although the enhancement by NCOA6 was not quite significant (p = 0.09). NCOA6 by itself does not transactivate the CYP2C9 promoter. As a positive control, expression of PGC-1, a known coactivator of HNF4 (which is known to be expressed at low levels in HepG2 cells), significantly increased HNF4 activation (p < 0.01) to 12-fold. PGC-1 had no effect on the 2C9 promoter activity alone (data not shown). When both CAR and HNF4 are coexpressed with the CYP2C9 promoter in the presence of the CAR ligand CITCO, there is a synergistic 26-fold transactivation of CYP2C9 promoter activity when compared with 11-fold activation by CAR alone or 4.8-fold by HNF4 alone (Fig. 4c). Cotransfection with NCOA6 slightly increased this activation by CAR and HNF4 to 35-fold, whereas PGC-1 produced no significant increase in this experiment. In summary, there is a synergistic activation of CYP2C9 promoter expression by the nuclear receptors CAR and HNF4 in the presence of the CAR ligand, CITCO. Exogenous NCOA6 had no effect on CAR activation but modestly enhanced the activation of the CYP2C9 promoter by HNF4 and the synergistic activation by CAR and HNF4, showing that it is a coactivator of HNF4.
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. To more definitely test the transcriptional role of NCOA6 in the synergistic activation of CYP2C9 by CAR and HNF4, we then expressed siRNA directed against NCOA6 in HepG2 cells using adenoviral system to silence endogenous NCOA6 expression. Among five targets tested, NC-V was not effective. Of the remaining four, NC-III and NC IV reduced the expression of NCOA6 mRNA levels by 95 and 88%, respectively, as quantified by qPCR (p < 0.001) (Fig. 5a). NC-III produced a significantly lower expression than any of the other targets. Western blot analysis confirmed that NCOA6 protein was markedly decreased in cells infected with NC-III (Fig. 5c), whereas PGC-1 and Pol II remain unchanged. To test whether silencing of NCOA6 affects the ligand-dependent synergistic activation of the CYP2C9 promoter by CAR and HNF4, the CYP2C9-luc promoter construct and CAR, HNF4, or CAR-HNF4 were transiently transfected into HepG2 cells; 24 h later, the cells were infected with adenovirus expressing scrambled or siRNAs for NCOA6. It should be noted that the magnitude of the synergistic activation is larger than in Fig. 4c, probably because transfection efficiencies are different during culture involving adenoviral infection (serum is absent from the medium but is added later). Serum is absent from the medium during adenoviral treatment of the cells, but is added later which would shock the cells altering transfection efficiency. The synergistic activation of the CYP2C9 promoter by CAR-HNF4 was dramatically suppressed by the expression of siRNA (NC-III) for NCOA6 in the presence and absence of ligand (Fig. 5b).
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synergistically activate the CYP2C9 promoter and that the down-regulation of NCOA6 levels greatly inhibits this synergistic activation, we examined whether there was any synergistic induction of CYP2C9 mRNA by CAR and HNF4, as well as the effect of siNCOA6 (NC-III) on the synergistic increase in CYP2C9 mRNA in vivo. We infected AdCAR and AdHNF4 individually and in combination in HepG2 cells and simultaneously down-regulated NCOA6 levels with siRNA for NCOA6 (NC-III). Adenoviral expression of CAR and HNF4 produces a synergistic rather than additive 1000-fold induction of CYP2C9 mRNA in HepG2 cells compared with a 100-fold induction by HNF4 alone and a 35-fold induction by CAR alone. The synergistic effects of HNF4 and CAR on CYP2C9 mRNA in HepG2 cells were abolished by down-regulating NCOA6 with adenoviral siRNA (p < 0.01) (Fig. 6b). In contrast, silencing NCOA6 did not affect the induction of CYP2C9 mRNA by CAR alone or HNF4 alone (data not shown). Silencing of NCOA6 also did not affect the expression of mRNA of other known coactivators such as SRC-1, PBP, or GRIP-1, although siRNA for NCOA6 modestly decreased mRNA for PGC-1 (40%) (data not shown). Taken together, the effects of NCOA6 siRNA on the synergistic transactivation by CAR and HNF4 and their mRNAs strongly indicate that NCOA6 acts as a bridging partner between CAR and HNF4 responsible for the synergistic up-regulation of CYP2C9 gene expression.
Recruitment Analysis of CAR, HNF, and Cofactors on the CAR Response Element and HNF4 Response Element of CYP2C9 Promoter. ChIP assays were performed on the chromatin extracts from the HepG2 cells described above in Fig. 6b to demonstrate the recruitment of nuclear receptors CAR and HNF4 to their respective binding sites on CYP2C9 promoter and the effect of silencing of NCOA6 on the association of coactivators to these nuclear receptors (Fig. 7). The following PCR products were generated by primer pairs for the CAR-RE (Fig. 7a), HNF4-RE (Fig. 7b), or for a negative control primers from glyceraldehyde-3-phosphate dehydrogenase-CNAP-1 gene (Fig. 7c). Input chromatin and nonimmune IgG was used as a negative control are shown at the bottom. These ChIP assays demonstrate that CAR is recruited robustly to the CAR binding site (Fig. 7a) on the promoter region of CYP2C9 in chromatin extracts prepared from HepG2 cells, particularly in cells overexpressing CAR and moderately in cells overexpressing CAR-HNF4 with or without the siRNA for NCOA6 (NC-III). As expected, HNF4 was recruited to its binding site (Fig. 7b) on the CYP2C9 promoter in all of the chromatin extracts from cells overexpressing HNF4, CAR-HNF4, and siRNA for NCOA6 (NC-III) did not affect this recruitment. Antibodies for the coactivator NCOA6 robustly precipitated the HNF4 binding sites in chromatin extracts from cells overexpressing CAR, HNF4, and CAR-HNF4, particularly in cells overexpressing both CAR and HNF4, and also precipitated the CAR binding site, although less robustly. This association of NCOA6 to both the HNF4 binding sites and CAR binding site was negligible in chromatin extracts from cells expressing NCOA6 siRNA. The coactivator PGC-1 was robustly associated with both the HNF4 binding sites and CAR binding sites in chromatin extracts from cells overexpressing CAR, HNF4, and CAR-HNF4, whereas PIMT was associated primarily with the HNF4 site. Silencing of NCOA6 with NC-III greatly reduced or abolished the association of CBP, PGC-1, and PIMT to the CYP2C9 promoter, suggesting that NCOA6 is required for the recruitment of cofactors from the mediator complex. Fig. 7c shows no amplification of a nontarget gene from immunoprecipitated chromatins. Thus, ChIP assays show that CAR and HNF4 interact with their respective binding sites on the CYP2C9 promoter in vivo in its native context, NCOA6 interacts with both the nuclear receptors and brings down both sites by its interaction with the nuclear receptors CAR and HNF4, and silencing NCOA6 prevents the recruitment not only of NCOA6 but also other coactivators to the HNF4-RE.
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Discussion |
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TopAbstractMaterials and MethodsResultsDiscussionReferences |
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. It is noteworthy that we identify NCOA6 as a new HNF4-interacting protein using the HNF4 as the bait in yeast two-hybrid screening. To identify the proteins that interact with full-length HNF4, we used a GST pull-down approach using nuclear extracts from HepG2 cells expressing CAR. Both CAR and CYP2C9 are expressed at very low levels in HepG2 cells (Ferguson et al., 2002), but overexpression of CAR (by infection with AdCAR) induces CYP2C9 mRNA expression in these cells. Therefore, we used nuclear extracts from HepG2 cells infected with AdCAR to identify binding protein partners in the CAR-HNF4 complex. Mass spectral analysis identified CAR, and immunoblotting identified NCOA6 and CAR as well as CBP, PGC-1, and PIMT in this mega complex. As discussed earlier, nuclear receptor coactivators exist in subcomplexes (McKenna et al., 1999). Although there are two schools of thought regarding whether these subcomplexes form sequentially or combinatorially, recent evidence points to proteins that facilitate such linkage of these subcomplexes, even for a transient period (Misra et al., 2002). Examples of such proteins include NCOA6, linking CBP to a PBP complex, and PIMT, directly linking CBP with PBP. Consistent with this bridging function, we found proteins belonging to the known CBP subcomplex and mediator subcomplex in the isolated CAR-HNF4 complex from HepG2 cells. The synergistic activation of HNF4 and CAR on CYP2C9 promoter activity, the effects of silencing NCOA6 on this synergism, and the results of ChIP assays suggest that after recruitment of CAR and HNF4 to their respective binding sites, a set of coactivators are recruited to form a bridge between the receptors initiating the observed surge in transcriptional activity. siRNA studies suggest that NCOA6 is necessary for the formation of this bridge between HNF4 and CAR and for recruitment of other coactivators to the proximal HNF4 sites.
PBP (Zhu et al., 1997) and PRIP/NCOA6 (Zhu et al., 2000) are known to regulate individually the expression of the ap2 gene, which is involved in adipogenesis (Qi et al., 2003). GRIP-1 and PBP have also been shown to function as coactivators for a CAR-mediated increase in Cyp2b10 gene expression (Min et al., 2002; Jia et al., 2005). Although NCOA6 has shown to be a coactivator for CAR (Choi et al., 2005), CAR-mediated gene expression of Cyp2b10 was not affected in NCOA6-null mice (Guo et al., 2006; Sarkar et al., 2007). GRIP-1 belongs to the p160 family of coactivators (Leo and Chen, 2000). Although GRIP-1 could interact with CAR and HNF4 individually, GRIP-1 is not a major player in bridging function. Deletion of the SRC-1, steroid receptor coactivator-2/GRIP-1, and SRC-3/pCIP genes in knockout mice have no effect on CAR-regulated gene transcription (Xia et al., 2007). PBP, on the other hand, acts as a coactivator for CAR, and studies in PBP-null mice indicate that PBP is necessary for the translocation of CAR to the nucleus, and PBP regulates the hepatic expression of CAR (Jia et al., 2005). In the present study, we show that NCOA6 acts as an interacting partner for HNF4 (Fig. 2). Because in vitro data show that PBP can bind to CAR, and HNF4 binds to NCOA6 (Fig. 2), we considered the possibility of a bridge formation either directly or through PIMT (Zhu et al., 2001). PIMT has been reported to form a bridge between NCOA6 and PBP (Misra et al., 2002).
Although there is also a possibility of an indirect interaction/mechanism between CAR and HNF4 with PIMT or PIMT-like methyl transferases, one likely scenario for the CAR-HNF4 bridge formation is by the direct interaction of NCOA6 with both of the receptors simultaneously. The nuclear receptor interacting box containing the first LXXLL motif is found to interact with CAR alone (Fig. 5b), whereas HNF4 interacted with the NR boxes coding for both the first LXXLL and the second LXXLL motifs (Fig. 5c). This opens the possibility that the nuclear receptor interacting box binds to CAR and the NR-2 box binds to HNF4, thereby directly bridging CAR and HNF4 to bring about the synergistic activation of the CYP2C9 promoter and the induction of CYP2C9 mRNA. Nonredundant coactivators like CBP and its binding proteins (histone acetylating proteins) are known to interact with both CAR and HNF4; likewise, the redundant coactivator PGC-1 (a member of the PGC-1 family known to be involved in splicing) interacts with both CAR and HNF4. Although these coactivators are probably part of the CAR-HNF4 transcription complex, our data suggest that it is unlikely that they are required for the bridge between CAR and HNF4. For example, the redundant coactivator PGC-1 could rescue not the silencing effect of NCOA6 in CYP2C9 transactivation assays.
Promoter regions of genes have binding sites for numerous nuclear proteins and nuclear receptors. Therefore, the possibility of cross-talk between binding partners is not only feasible but essential to transduce complex and sometimes contradictory signals for the regulation of a gene. Such a cross-talk between glucocorticoid receptor and peroxisome proliferator-activated receptor- has been documented on ap2 gene expression and between the coactivators PBP and NCOA6 (Qi et al., 2003). CAR also cross-talks with the fork-head transcription factor FOXO1 to repress its activation of an insulin response sequence in the glucose-6-phosphatase gene (Kodama et al., 2004). PXR also cross-talks with the insulin response transcription factor FoxA2 to repress the transcription of genes involved in lipid metabolism in the mouse (Nakamura et al., 2007), and PXR represses glucagon activation of the glucose-6-phosphatase gene by binding to CREB, the cAMP response element binding protein, inhibiting CREB interaction with its DNA binding elements (Kodama et al., 2007). Several examples of either inhibitory cross-talk or potentiation between HNF4 and PXR or CAR have been reported. PXR seems to interfere with HNF4-up-regulation of CYP7A1 and the regulation of cholesterol metabolism (Bhalla et al., 2004; Li et al., 2007b). HNF4 has long been suggested to have a positive role in the PXR-mediated induction of CYP3A4. Possible potentiation between HNF4 and CAR has also been reported recently in the regulation of CYP3A4 (Li and Chiang, 2006) and steroid and bile acid sulfotransferase (SULT2A1) (Echchgadda et al., 2007).
Our study provides evidence for a new mechanism for the synergistic effect of HNF4 with CAR on expression of the human CYP2C9 gene, wherein cofactors are proposed to bridge distant receptor sites in the promoter, resulting in a synergistic effect on gene expression. In particular, NCOA6 seems to be an essential factor, possibly providing a platform for the recruitment of cofactors to this bridge.
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ABBREVIATIONS: CAR, constitutive activate/androstane receptor; HNF4, hepatocyte nuclear factor 4; RE, response element; NCOA6, Nuclear receptor coactivator 6 (PRIP/RAP250/ASC-2); PBP, peroxisome proliferator-activated receptor binding protein (TRAP220/MED-1/DRIP205); CBP, cAMP response element-binding protein binding protein; PCR, polymerase chain reaction; SRC-1, steroid receptor coactivator-1; PGC-1, peroxisome proliferator-activated receptor coactivator-1; PIMT, PRIP-interacting protein with methyltransferase domain; GRIP-1, glucocorticoid receptor interacting protein; ChIP, chromatin immunoprecipitation; qPCR, quantitative polymerase chain reaction; CITCO, 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime; PXR, pregnane X receptor; CREB, cAMP response element-binding protein; aa, amino acid; siRNA, small interfering RNA; bp, base pair(s); TEGN, Tris, EDTA, glycerol, and Nonidet P-40; PAGE, polyacrylamide gel electrophoresis; NIEHS, National Institute of Environmental Health Sciences; VP, virus particles; Ad, adenovirus; MS/MS, tandem mass spectrometry.
Address correspondence to: Dr. Joyce A. Goldstein, Laboratory of Pharmacology (A3-02), NIEHS, P.O. Box 12233, Research Triangle Park, NC 27709. E-mail: goldste1{at}niehs.nih.gov
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) and absence (
) of 100 nM CITCO as the ligand with pRL as the internal control. All transfections were performed in triplicate. Values are means of triplicates ± S.E. CAR significantly up-regulates the CYP2C9 promoter in the absence and presence of ligand at *, p < 0.05; **, p < 0.01; ***, p < 0.001. Transfection with PGC-1
