Rational Engineering of Human Cytochrome P450 2B6 for Enhanced Expression and Stability: Importance of a Leu²⁶⁴Phe Substitution

Data Supplement

Files in this Data Supplement:

• Data Supplement -
  Table S1: Oligonucleotides used in PCR for site-directed mutagenesis of P450 2B6dH

  Figure S1: A. Thermal inactivation of P450 2B enzymes … B. Temperature sensitivity profiles of the 2B enzymes.

  Figure S2: Typical transition profile of temperature-induced protein denaturation monitored by protein fluorescence…

  Figure S3: Temperature sensitivity profiles of the 2B6dH mutants…

  Figure S4: P450-heme depletion assays at…

  Figure S5: A. Typical equilibrium spectra of GuHCl induced heme dissociation of P450 (example 2B6dH L264F). B. The differential absorbance data were fit to two-state transition to obtain the mid pint concentration GuHCl1/2.