MolPharm

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

Molecular Pharmacology Fast Forward
First published on June 6, 2005; DOI: 10.1124/mol.105.014670

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
mol.105.014670v1
68/3/793    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tavernier, G.
Right arrow Articles by Langin, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tavernier, G.
Right arrow Articles by Langin, D.


Received for publication May 16, 2005.
Revised June 6, 2005.
Accepted for publication June 6, 2005.

Norepinephrine Induces Lipolysis in {beta}1/{beta}2/{beta}3 -adrenoceptor Knockout Mice

Genevieve Tavernier 1*, Maria Jimenez 2, Jean-Paul Giacobino 2, Nicolas Hulo 3, Max Lafontan 1, Patrick Muzzin 2, Dominique Langin 1

1 Inserm U586, Toulouse 2 Universite de Geneve 3 Swiss Institute of Bioinformatics, Geneve

* Address correspondence to: E-mail: getaver{at}toulouse.inserm.fr

Abstract

Catecholamines are major stimulants of adipose tissue metabolism. Norepinephrine and epinephrine act through three subtypes of {beta}-adrenoceptors ({beta}-AR) expressed in the adipocytes. The aim of this work was to study the mechanisms of lipid mobilization in {beta}1/{beta}2/{beta}3-AR triple knockout ({beta}-less) mice. Glycerol and non-esterified fatty acids released from isolated adipocytes were measured as an index of lipolytic activity. There was no difference between the two genotypes for basal lipolysis and lipolytic response to adrenocorticotropic hormone or to agents acting at the adenylyl cyclase and protein kinase A levels. The lipolytic response to norepinephrine and {beta}-AR agonists was blunted in {beta}-less mice. However, a residual low affinity lipolytic effect was observed in the presence of catecholamines and {beta}3-AR agonists but not of {beta}1- or {beta}2-AR agonists. cAMP levels were increased by a {beta}-AR agonist in white and brown adipocytes of {beta}-less mice. The residual lipolytic effect was blocked by {beta}-AR antagonists. It was mediated neither by {alpha}1- or {alpha}2-AR nor by dopaminergic, serotoninergic and histaminergic receptors. Bioinformatic analyses do not provide evidence for a fourth {beta}-AR. We conclude that the residual lipolytic effect observed in {beta}-less mice can be attributed to an unknown Gs-protein-coupled receptor with low affinity for catecholamines.


Key words: Adrenergic, Gs family, cAMP


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
T. J. Bartness and C. K. Song
Thematic review series: Adipocyte Biology. Sympathetic and sensory innervation of white adipose tissue
J. Lipid Res., August 1, 2007; 48(8): 1655 - 1672.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Miura, K. Kawanaka, Y. Kai, M. Tamura, M. Goto, T. Shiuchi, Y. Minokoshi, and O. Ezaki
An Increase in Murine Skeletal Muscle Peroxisome Proliferator-Activated Receptor-{gamma} Coactivator-1{alpha} (PGC-1{alpha}) mRNA in Response to Exercise Is Mediated by {beta}-Adrenergic Receptor Activation
Endocrinology, July 1, 2007; 148(7): 3441 - 3448.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2005 by the American Society for Pharmacology and Experimental Therapeutics