Received for publication May 11, 2005.
Revised August 31, 2005.
Accepted for publication September 1, 2005.

Stereo-specific Induction of NF-B Activation by Isochamaejasmin

Abstract

The root of Stellera chamaejasme L. is a traditional Chinese herb termed Rui Xiang Lang Du and has been used to treat solid tumors, tuberculosis and psoriasis. Exactly how Stellera chamaejasme L. regulates cellular responses remains unclear. We examined four biflavonoids isolated from Stellera chamaejasme L., including isochamaejasmin, two of its stereo-isomers and a methyl derivative, in functional assays originally designed to screen ligands for the G protein-coupled formyl peptide receptor-like 1 (FPRL1). Isochamaejasmin was found to induce the expression of a NF-B-directed reporter gene in transfected HeLa cells with an EC₅₀ of 3.23 mM, independently of FPRL1. The isochamaejasmin-stimulated NF-B reporter activity was accompanied by nuclear translocation of NF-B proteins and was blocked by a dominant negative construct of IB. Isochamaejasmin also induced time-dependent phosphorylation of the mitogen-activated protein kinases ERK1/2 and p38, and a novel protein kinase C, PKC. Likewise, inhibition of these kinases with the respective pharmacological inhibitors significantly reduced the isochamaejasmin-stimulated NF-B activation. Interestingly, the two stereo-isomers and the methyl derivative did not induce detectable activation of NF-B and were more cytotoxic than isochamaejasmin, which could partially rescue cycloheximide-induced apoptosis. Inhibition of NF-B activation reversed the anti-apoptotic effect of isochamaejasmin. These results provide the first evidence for a potential mechanism of action by Stellera chamaejasme L., and indicate that structurally similar compounds derived from Stellera chamaejasme L. may have different pharmacological properties.

Key words: Protein Kinase C, Sequestration/Internalization, NFkappaB, Structure-activity relationships and modeling, Apoptosis

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