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Received for publication June 9, 2006.
Revised January 30, 2007.
Accepted for publication January 31, 2007.
ABSTRACT The dopamine transporter (DAT) terminates dopamine (DA) neurotransmission by reuptake of DA into presynaptic neurons. Regulation of DA uptake by D2 dopamine receptors (D2R) has been reported. The high affinity of DA and other DAT substrates for the D2R, however, has complicated investigation of the intracellular mechanisms mediating this effect. The present studies used the fluorescent DAT substrate, 4-[4-(diethylamino)-styryl]-N-methylpyridinium iodide (ASP+) with live cell imaging techniques to identify the role of two D2R-linked signaling pathways, extracellular signal-regulated kinases 1 and 2 (ERK 1/2) and phosphoinositide 3 kinase (PI3K), in mediating D2R regulation of DAT. Addition of the D2/D3 receptor agonist quinpirole (0.1-10 µM) to human embryonic kidney cells co-expressing human DAT and D2 receptor (short splice variant, D2SR) induced a rapid, concentration-dependent and pertussis toxin-sensitive increase in ASP+ accumulation. The D2/D3 agonist, PD128907 also increased ASP+ accumulation. D2SR activation increased phosphorylation of ERK 1/2 and Akt, a major target of PI3K. The MEK inhibitor, PD98059, prevented the quinpirole-evoked increase in ASP+ accumulation, whereas inhibition of PI3K was without effect. Fluorescence flow cytometry and biotinylation studies revealed a rapid increase in DAT cell-surface expression in response to D2R stimulation. These experiments demonstrate that D2SR stimulation increases DAT cell surface expression and therefore enhances substrate clearance. Furthermore, they show that the increase in DAT function is ERK 1/2-dependent but PI3K-independent. Our data also suggest the possibility of a direct physical interaction between DAT and D2R. Together, these results suggest a novel mechanism by which D2SR autoreceptors may regulate DAT in the CNS.
Key words:
Dopamine, MAP Kinase, Biogenic Amine, Fluorescence techniques
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