Abstract
Evidence suggests that the long-term adaptations in the hippocampus after repeated drug treatment may parallel its role during memory formation. The neuroplasticity that subserves learning and memory is also believed to underlie addictive processes. We have reported previously that repeated morphine administration alters local distribution of endocytic proteins at hippocampal synapses, which could in turn affect expression of glutamate receptors. Glutamatergic systems, including α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs), are believed to be involved in opiate-induced neuronal and behavioral plasticity, although the mechanisms underlying these effects are only beginning to be understood. The present study further examines the effects of repeated morphine administration on the expression and composition of AMPARs and the functional ramifications. Twelve hours after the last morphine injection, we observed an increased expression of AMPARs lacking glutamate receptor (GluR) 2 in hippocampal synaptic fractions. Immunoblotting studies show that 12 h after morphine treatment, GluR1 subunits are increased at the postsynaptic density (PSD) and at extrasynaptic sites, whereas GluR3 subunits are only increased at the PSD, and they show how this alters receptor subunit composition. In addition, we provide electrophysiological evidence that AMPARs are switched to Ca2+-permeable (GluR2-lacking) at the synapse 12 h after repeated morphine treatment, affecting the magnitude of long-term depression at hippocampal neurons. We propose that morphine-induced changes in glutamatergic synaptic transmission in the hippocampus may play an important role in the neuroadaptations induced by repeated morphine administration.
Footnotes
This work was supported by the National Institutes of Health National Institute on Drug Abuse [Grants R03-DA023454, R01-DA025036].
Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org.
doi:10.1124/mol.109.060301.
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ABBREVIATIONS:
- VTA
- ventral tegmental area
- AMPA
- α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid
- AMPAR
- α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor
- PSD
- postsynaptic density
- LTP
- long-term potentiation
- LTD
- long-term depression
- fEPSP
- extracellular field excitatory postsynaptic potential
- EPSC
- excitatory postsynaptic currents
- HFS
- high-frequency stimulation
- LFS
- low-frequency stimulation
- d-APV
- 2-amino-5-phosphonovalerate
- RI
- rectification index
- Phtx
- philanthotoxin
- JST
- Joro spider toxin
- NMDA
- N-methyl-d-aspartate
- NMDAR
- N-methyl-d-aspartate receptor
- PTX
- picrotoxin
- ANOVA
- analysis of variance
- IP
- immunoprecipitation
- FV
- fiber volley
- PPF
- paired-pulse facilitation
- CGP55845
- (2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl)phosphinic acid hydrochloride
- QX314
- N-(2,6-dimethylphenylcarbamoylmethyl)triethylammonium
- ACSF
- artificial cerebral spinal fluid.
- Received August 13, 2009.
- Accepted February 16, 2010.
- Copyright © 2010 The American Society for Pharmacology and Experimental Therapeutics
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