Abstract
Normal human plasma and serum have been shown to contain two active species of dopamine β-hydroxylase (EC 1.14.17.1) by gradient ultracentrifugation and gel filtration. About 75-80% of the dopamine β-hydroxylase activity in plasma sediments almost exactly like bovine dopamine β-hydroxylase, and has a molecular weight of 289,000. The remainder of the dopamine β-hydroxylase activity in plasma or serum (20-25%) has a molecular weight of 147,000, or one-half that of the principal species. No interconversion is observed between these two species at pH 7.4, ionic strength 0.15. Thus a significant amount of dopamine β-hydroxylase activity in plasma is in the form of an active dimer of the basic subunit of this enzyme.
- Copyright © 1977 by Academic Press, Inc.
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