Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • For Subscribers
    • For Advertisers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • For Subscribers
    • For Advertisers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Antagonism of Carbamylcholine-Induced Depolarization by Batrachotoxin and Veratridine

D. L. GARRISON, E. X. ALBUQUERQUE, J. E. WARNICK, J. W. DALY and B. WITKOP
Molecular Pharmacology January 1978, 14 (1) 111-121;
D. L. GARRISON
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
E. X. ALBUQUERQUE
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. E. WARNICK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. W. DALY
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
B. WITKOP
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Depolarization of muscle end plates by carbamylcholine was reduced by 35-64% in muscles that had been treated with batrachotoxin or veratridine. Tetrodotoxin was used to prevent or reverse the membrane depolarization elicited by batrachotoxin or veratridine in the surface fibers of muscles, prior to the addition of carbamylcholine. But even when tetrodotoxin was added initially to prevent any depolarization of the muscle membrane by batrachotoxin, the latter toxin retained its inhibitory effect on the carbamylcholine response. Treatment with tetrodotoxin alone or tityustoxin followed by tetrodotoxin had no effect on the end plate depolarization induced by carbamylcholine. The inhibitory effect of veratridine on the response to carbamylcholine, but not that of batrachotoxin, was reversible. Prior treatment with batrachotoxin reduced responses to a bath-applied combination of acetylcholine plus neostigmine as well, while having no effect on responses to microiontophoretically applied acetylcholine, on miniature end plate potentials, or on end plate currents. The muscle depolarization elicited by batrachotoxin was unaffected by α-bungarotoxin, an acetylcholine receptor antagonist, or by histrionicotoxin, an antagonist of the ion conductance modulator associated with the acetylcholine receptor. The ability of batrachotoxin to alter the end plate response to carbamylcholine was found to be noncompetitive. The results are consonant with activation of tetrodotoxin-insensitive sodium channels by batrachotoxin and veratridine and the subsequent lack of participation of these activated channels in the depolarization of end plates by carbamylcholine. The depolarization elicited by carbamylcholine in a batrachotoxin-treated preparation thus reflects only the activation of acetylcholine receptor-ion conductance modulator complexes.

ACKNOWLEDGMENTS The authors express their appreciation to Miss Mabel Alice Zelle for excellent computer assistance and Mrs. Maria Luisa Diniz de Oliveira for secretarial assistance.

  • Copyright © 1978 by Academic Press, Inc.

Log in using your username and password

Forgot your user name or password?

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 14, Issue 1
1 Jan 1978
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Antagonism of Carbamylcholine-Induced Depolarization by Batrachotoxin and Veratridine
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
Citation Tools
Research ArticleArticle

Antagonism of Carbamylcholine-Induced Depolarization by Batrachotoxin and Veratridine

D. L. GARRISON, E. X. ALBUQUERQUE, J. E. WARNICK, J. W. DALY and B. WITKOP
Molecular Pharmacology January 1, 1978, 14 (1) 111-121;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Research ArticleArticle

Antagonism of Carbamylcholine-Induced Depolarization by Batrachotoxin and Veratridine

D. L. GARRISON, E. X. ALBUQUERQUE, J. E. WARNICK, J. W. DALY and B. WITKOP
Molecular Pharmacology January 1, 1978, 14 (1) 111-121;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • BODIPY-cyclopamine Binding to Nluc-SMO
  • Operational Models for Receptors with Multiple Agonist Sites
  • Characterization and optimization of the novel TRPM2 antagonist tatM2NX
Show more Article

Similar Articles

  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2019 by the American Society for Pharmacology and Experimental Therapeutics