Abstract
When ionic forces contribute to the binding of drugs by a protein the groups involved in this electrostatic bonding may undergo pK shifts. A pH titration method is described to detect such pK changes. The procedure is illustrated by the binding of sulfaethidole to human serum albumin. For this case changes in pK of groups both of the protein and of the bound drug are observed using this method.
ACKNOWLEDGMENTS We thank the technical service (head Mr. A. Dirksen) for constructing the titration equipment.
- Copyright © 1978 by Academic Press, Inc.
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