Abstract
Radiolabeled 3-amino-8-azido-5-ethyl-[14C]-6-phenyl phenanthridine (ethidium monoazide) was photolyzed in situ in the presence of calf thymus DNA, RNA, poly A, calf thymus histones and nucleoid structures prepared from lymphocytes. Non-linear least squares analysis of the binding data enabled us to predict the amount of drug adduct formed at infinite polymer concentration. Photolytic efficiency could the be calculated since the input drug concentration was constant and therefore represented the non-covalent complex expected at infinite polymer concentration. The efficiency of photolysis for native, heat-denatured and formaldehyde-denatured DNA was 0.23, 0.26 and 0.45, respectively, and for RNA it was found to be 0.29. The value for poly A was 0.42. The intensity of light was not a limiting factor under the conditions of these experiments.
- Copyright © 1979 by Academic Press, Inc.
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