Abstract
Poly(rI) and poly(rC) can each be wrapped with a colloidal complex of low viscosity carboxymethylcellulose and polylysine, without formation of precipitates, to form complexes containing 10 mg/ml polynucleotide material. From its resistance to ribonuclease treatment and inability to anneal to poly(rC) we conclude that the carboxymethylcellulose polylysine wrapped poly(rI) is really wrapped. Migration of wrapped poly(rI) and wrapped poly(rC) in agarose gel electrophoresis demonstrated that the negative charges of the polynucleotides were neutralized by wrapping. The double-stranded polynucleotide, poly(rI)·poly(rC), could also be wrapped by the same procedure but only to a final polynucleotide concentration of 2 mg/ml. The wrapping procedure should be effective for any polynucleotide. Mixtures of equal masses of wrapped poly(rI) and wrapped poly(rC) do not produce detectable serum interferon concentrations in mice when both polynucleotides are administered at 25 µg/mouse or less while wrapped poly(rI)·poly(rC) produces high concentrations of serum interferon. However, at equal polynucleotide doses the wrapped single-stranded polynucleotide preparations can produce the same degree of resistance to infection with encephalomyocarditis virus as wrapped poly(rI)·poly(rC).
ACKNOWLEDGMENTS We thank Dr. N. H. Carey for critical examination of the manuscript; I.J.D. Lindley for assistance with mouse experiments; Janet Witney for agarose gel analyses; and Dr. A.J. Hale for providing the research facilities that made these studies possible.
- Copyright © 1979 by Academic Press, Inc.
MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|