Abstract
The addition of 0.5 mM adenosine to keratomed epidermal strips obtained from human volunteers or mice significantly increased the level of adenosine 3':5'-monophosphate (cyclic AMP) in the tissue. The increase was dose dependent between 0.05 mM and 0.01 M adenosine. Theophylline decreased the adenosine-induced increase in cyclic AMP, while other cyclic nucleotide phosphodiesterase inhibitors such as papaverine or Ro 20-1724 augmented the increase. Adenosine 5'-monophosphate but not adenine increased the level of cyclic AMP in the epidermis. Epidermal basal cells in primary culture responded to the addition of adenosine and dipyridimole with a threefold increase in the cyclic AMP concentration in the cells. Adenosine increased the cyclic AMP levels in lesional and uninvolved areas of epidermis obtained from psoriatic patients.
ACKNOWLEDGMENTS The primary epidermal cell cultures provided by Dr. Cynthia Marcelo and the excellent technical expertise of Ms. Wendy Bazner are gratefully acknowledged.
- Copyright © 1980 by The American Society for Pharmacology and Experimental Therapeutics
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