Abstract
Using a technique based on the relaxation phenomenon of nuclear magnetic resonance, the interaction of a drug and its receptor site has been directly observed in an intact cellular system. The NMR spectrum of epinephrine is altered by the presence of liver cells in a manner characteristic of a binding interaction. The spectral changes permit certain conclusions to be drawn about time nature of the binding. Dichloroisoproterenol, a beta-adrenergic blocking agent, competitively inhibits the changes in the epinephrine spectrum while its own spectrum exhibits evidence of binding.
ACKNOWLEDGMENT We would like to thank Dr. Catherine Rappaport for help in preparing the isolated liver cells.
- Copyright ©, 1969, by Academic Press Inc.
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