Abstract
The localization of an epitope-tagged receptor for thyrotropin-releasing hormone (TRH) expressed in different cell contexts was studied with immunofluorescence microscopy. In pituitary lactotrophs, which normally express TRH receptors, and in AtT20 pituitary corticotrophs, TRH receptor immunoreactivity was primarily confined to the plasma membrane. In HEK 293 and COS7 cells, TRH receptors were predominantly intracellular. In transiently transfected COS7 cells, the TRH receptor colocalized with endoplasmic reticulum and Golgi markers. The pattern of TRH receptor immunofluorescence was the same over a wide range of receptor expression in transiently transfected COS7 cells, and all cell lines bound similar amounts of3H- and rhodamine-labeled TRH analogs, suggesting that cell-specific differences in TRH receptor localization were not simply the result of overexpression. In all cell contexts, TRH receptors on the plasma membrane underwent extensive ligand-driven endocytosis. Inhibitors of glycosylation did not alter the subcellular distribution of receptors. In HEK 293 cells expressing the transfected TRH receptor, protein synthesis inhibitors caused translocation of intracellular receptors to the cell surface, as shown by a marked increase in cell surface immunofluorescence and [3H][N3-methyl-His2]TRH binding. These results demonstrate that the subcellular localization of the TRH receptor depends on the cell context in which it is expressed and that intracellular receptors are capable of translocation to the plasma membrane.
Footnotes
- Received September 3, 1996.
- Accepted January 17, 1997.
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Send reprint requests to: Dr. P. M. Hinkle, Box 711, Department of Pharmacology & Physiology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642. E-mail:hinklep{at}pharmacol.rochester.edu
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This work was supported in part by National Institutes of Health Grant DK19974 and Cancer Center Core Research Grant CA11098 and by a Pharmaceutical Manufacturers’ Association Advanced Predoctoral Fellowship and Wilmot Fellowship (R.Y.).
- The American Society for Pharmacology and Experimental Therapeutics
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