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Research ArticleArticle

Receptor-Mediated Activation of Gsα: Evidence for Intramolecular Signal Transduction

Stephen R. Marsh, Galina Grishina, Paul T. Wilson and Catherine H. Berlot
Molecular Pharmacology June 1998, 53 (6) 981-990;
Stephen R. Marsh
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Galina Grishina
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Paul T. Wilson
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Catherine H. Berlot
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Abstract

To investigate the mechanism by which cell surface receptors activate heterotrimeric G proteins, we applied a scanning mutagenesis approach to the carboxyl-terminal 40% of αs (residues 236–394) to identify residues that play a role in receptor-mediated activation. We identified four regions of sequence in which mutations significantly impaired receptor-dependent stimulation of cAMP synthesis in transiently transfected cyc− S49 lymphoma cells, which lack endogenous αs. Residues at the carboxyl terminus are likely to be receptor contact sites. Buried residues near the bound GDP are connected to the carboxyl terminus by an α helix and may regulate GDP affinity. Residues in two adjacent loops of the GTPase domain at the interface with the helical domain, one of which includes a region, switch III, that changes conformation on GTP binding, are positioned to relay the receptor-initiated signal across the domain interface to facilitate GDP release. Consistent with this hypothesis, replacing the helical domain of αs with that of αi2 in an αs/αi2/αs chimera corrects the defect in receptor-mediated activation caused by αi2substitutions on the GTPase side of the interface. Thus, complementary interactions between residues across the domain interface seem to play a role in receptor-catalyzed activation.

Footnotes

  • Send reprint requests to: Dr. Catherine H. Berlot, Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520-8026. E-mail:cathy_berlot{at}qm.yale.edu

  • This research was supported by National Institutes of Health Grant GM50369 to C.H.B.

  • Abbreviations:
    GTPγS
    guanosine-5′-O-(3-thio)triphosphate
    HEPES
    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
    HEK
    human embryonic kidney
    • Received November 25, 1997.
    • Accepted February 18, 1998.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 53 (6)
Molecular Pharmacology
Vol. 53, Issue 6
1 Jun 1998
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Research ArticleArticle

Receptor-Mediated Activation of Gsα: Evidence for Intramolecular Signal Transduction

Stephen R. Marsh, Galina Grishina, Paul T. Wilson and Catherine H. Berlot
Molecular Pharmacology June 1, 1998, 53 (6) 981-990;

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Research ArticleArticle

Receptor-Mediated Activation of Gsα: Evidence for Intramolecular Signal Transduction

Stephen R. Marsh, Galina Grishina, Paul T. Wilson and Catherine H. Berlot
Molecular Pharmacology June 1, 1998, 53 (6) 981-990;
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