Increased Conversion of Tyrosine to Catecholamines in the Intact Rat Following Elevation of Tissue Tyrosine Hydroxylase Levels by Administered Phenoxybenzamine

Abstract

When phenoxybenzamine (20 mg/kg) was administered to rats for 2 consecutive days, a 2-fold increase in tryosine hydroxylase relative to controls was observed in adrenal and heart homogenates. A 4-6-fold increase in the incorporation of label from ¹⁴C-L-tyrosine into catecholamines was observed following 2 days of phenoxybenzamine treatment, in comparison to a 2-3-fold increase following a single dose of the alpha-adrenergic blocking agent. The administration of a ganglionic blocking agent 2 hr prior to the administration of ¹⁴C-L-tyrosine completely prevented the increased incorporation of label into catecholamines following 1 day of phenoxybenzamine treatment, but caused only a partial reduction in the accelerated rate of conversion of tyrosine to catecholamines observed after 2 days of phenoxybenzamine treatment. These data are consistent with the concept that the increased tyrosine hydroxylase activity measured in tissue homogenates expresses itself physiologically as an acceleration of catecholamine synthesis in the intact animal.