Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • For Subscribers
    • For Advertisers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • For Subscribers
    • For Advertisers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

The Metabolism of Ribonucleic Acid in Rat Liver after Phenobarbital Administration

A. M. COHEN and R. W. RUDDON
Molecular Pharmacology September 1970, 6 (5) 540-547;
A. M. COHEN
Departments of Pharmacology and Oral Biology, The University of Michigan Medical and Dental Schools, Ann Arbor, Michigan 48104
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R. W. RUDDON
Departments of Pharmacology and Oral Biology, The University of Michigan Medical and Dental Schools, Ann Arbor, Michigan 48104
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Incorporation of orotic acid-5-3H (100-200 µCi) into hepatic nuclear and cytoplasmic RNA extracted from purified nuclei or cytoplasmic fractions and separated on linear sucrose gradients was determined after intraperitoneal administration of phenobarbital (75 mg/kg) to rats. Pulses of 1-, 2-, 4-, and 12-hr duration were administered 2 hr after injection of phenobarbital or 0.9% NaCl in order to examine alterations in cytoplasmic RNA metabolism early in the induction process, i.e., before any marked increase occurred in the levels of microsomal drug-metabolizing enzymes. The data indicated that there was a 20-30% increase above controls in the specific activity of 28 S and 18 S ribosomal RNA, which became apparent after a 2-hr pulse dose of labeled precursor administered 2 hr after phenobarbital treatment, and which was maintained for 12 hr. The augmented labeling appeared to be restricted to ribosomal RNA species. Analysis of nuclear RNA after pulses of ½ 1, 2, and 4 hr, given 2 hr after phenobarbital, indicated that there was no detectable increase in synthesis of ribosomal RNA or ribosomal RNA precursors. It is suggested that phenobarbital may enhance the stability of hepatic ribosomal RNA or increase the "processing" and transport of this substance to the cytoplasm, and that these events, which occur early in the induction process, may be involved in the elevation of microsomal oxidase activity observed after phenobarbital administration.

ACKNOWLEDGMENT The authors wish to thank Mrs. Cynthia Rainey for her excellent technical assistance and for her help in the preparation of the manuscript.

  • Copyright ©, 1970, by Academic Press Inc.

Log in using your username and password

Forgot your user name or password?

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 6, Issue 5
1 Sep 1970
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
The Metabolism of Ribonucleic Acid in Rat Liver after Phenobarbital Administration
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
Citation Tools
Research ArticleArticle

The Metabolism of Ribonucleic Acid in Rat Liver after Phenobarbital Administration

A. M. COHEN and R. W. RUDDON
Molecular Pharmacology September 1, 1970, 6 (5) 540-547;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Research ArticleArticle

The Metabolism of Ribonucleic Acid in Rat Liver after Phenobarbital Administration

A. M. COHEN and R. W. RUDDON
Molecular Pharmacology September 1, 1970, 6 (5) 540-547;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • hsa-miR-9-3p and hsa-miR-9-5p as Post-Transcriptional Modulators of DNA Topoisomerase IIα in Human Leukemia K562 Cells with Acquired Resistance to Etoposide
  • Operational Models for Receptors with Multiple Agonist Sites
  • BODIPY-cyclopamine Binding to Nluc-SMO
Show more Articles

Similar Articles

  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2019 by the American Society for Pharmacology and Experimental Therapeutics