In the above article [Zangar RC, Kimzey AL, Okita JR, Wunschel DS, Edwards RJ, Kim H, and Okita RT (2002) Mol Pharmacol61:892–904], there was an error underExperimental Procedures.
On page 893, under Microsome Incubation Reactions, the sentence describing the loading buffer should have read: “Reactions were terminated by addition of 50 ml of SDS-PAGE loading buffer [62.5 mM Tris, pH 6.8, 1% SDS, 11% glycerol, 370 mM bromphenol blue, and 5% (v/v) 2-mercaptoethanol] and heating to 98°C for 5 min.”
In the original sentence, the concentration reported for 2-mercaptoethanol was incorrect.
The authors regret this error and apologize for any confusion or inconvenience it may have caused.
- The American Society for Pharmacology and Experimental Therapeutics