Abstract
The intestinal H(+)/peptide cotransporter 1 (PepT1) plays a major role in nitrogen supply to the body by mediating intestinal absorption of di- and tripeptides. Previous studies have reported that in animal models of type 2 diabetes/obesity, PepT1 activity and expression were markedly reduced. This prompted us to investigate the effects of two antidiabetic drugs, rosiglitazone and metformin, on PepT1 activity/expression in a murine diet-induced obesity model. C57BL/6J male mice were fed a high-fat diet (HFD) or a standard chow for 6 weeks and then were treated for 7 days with metformin (250 mg/kg/day) and/or rosiglitazone (8 mg/kg/day). For in vitro studies, Caco-2 enterocyte-like cells were treated for 7 days with metformin (10 mM) and/or rosiglitazone (10 μM). A 7-day rosiglitazone treatment increased PepT1 activity and prevented the 2-fold HFD-induced reduction in PepT1 transport. Metformin alone did not modify PepT1 activity but counteracted rosiglitazone-induced PepT1-mediated transport. As with the in vivo studies, rosiglitazone treatment up-regulated PepT1 transport activity with concomitant induction of S6 ribosomal protein activation in vitro. Furthermore, metformin decreased PepT1 expression (mRNA and protein) and its transport activity. The effect of metformin was linked to a reduction of phosphorylated S6 ribosomal protein (active form) and of phosphorylated 4E-BP1 (inactive form), a translation repressor. These data demonstrate that two antidiabetic drugs exert opposite effects on the PepT1 transport function probably through direct action on enterocytes. In our type 2 diabetes/obesity model, rosiglitazone, a peroxisome proliferator-activated receptor-γ agonist compensated for the HFD-induced PepT1 down-regulation, whereas metformin reversed rosiglitazone activity at the translational level.
Footnotes
Article, publication date, and citation information can be found at http://molpharm.aspetjournals.org.
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ABBREVIATIONS:
- PepT1
- intestinal H(+)/peptide cotransporter 1
- AMPK
- 5′ AMP-activated protein kinase
- 4E-BP1
- eukaryotic translation initiation factor 4E-binding protein 1
- PPAR
- peroxisome proliferator-activated receptor
- PPRE
- peroxisome proliferator-activated receptor response elements
- mTOR
- mammalian target of rapamycin
- AICAR
- 5-aminoimidazole-4-carboxamide ribotide
- SC
- standard laboratory chow
- HFD
- high-fat diet
- Gly-Sar
- glycyl-sarcosine
- KRB
- Krebs-Ringer bicarbonate buffer
- Gly-Gly
- glycyl-glycine
- Gly-Pro
- glycyl-proline
- ELISA
- enzyme-linked immunosorbent assay
- TEER
- transepithelial electric resistance.
- Received May 29, 2011.
- Accepted November 22, 2011.
- Copyright © 2012 The American Society for Pharmacology and Experimental Therapeutics
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