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Research ArticleArticle

HSP90 regulation of P2X7 receptor function requires an intact cytoplasmic C-terminus

Keisuke Migita, Taku Ozaki, shuji Shimoyama, Junko Yamada, Yoshikazu Nikaido, Tomonori Furukawa, Yuko Shiba, Terrance M Egan and Shinya Ueno
Molecular Pharmacology June 14, 2016, mol.115.102988; DOI: https://doi.org/10.1124/mol.115.102988
Keisuke Migita
Fukuoka University;
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Taku Ozaki
Hirosaki University;
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shuji Shimoyama
Hirosaki University;
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Junko Yamada
Hirosaki University;
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Yoshikazu Nikaido
Hirosaki University;
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Tomonori Furukawa
Hirosaki University;
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Yuko Shiba
Hirosaki University;
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Terrance M Egan
Saint Louis University
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Shinya Ueno
Hirosaki University;
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Abstract

P2X7 receptors (P2X7Rs) are ATP-gated ion channels that display the unusual property of current facilitation during long applications of agonists. Here we show that facilitation disappears in chimeric P2X7Rs containing the C-terminus of the P2X2 receptor (P2X2R), and in a truncated P2X7R missing the cysteine-rich domain of the C-terminus. The chimeric and truncated receptors also show an apparent decreased permeability to NMDG+. The effects of genetically modifying the C-terminus on NMDG+ permeability were mimicked by pre-application of the HSP90 antagonist geldanamycin to the wild-type receptor. Further, the geldanamycin decreased the shift in the reversal potential of the ATP gated current measured under bi-ionic NMDG+/Na+ condition without effecting the ability of the long application of agonist to facilitate current amplitude. Taken together, the results suggest that HSP90 may be essential for stabilization and function of P2X7Rs through an action on the cysteine-rich domain of the cytoplasmic C-terminus.

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Molecular Pharmacology: 97 (1)
Molecular Pharmacology
Vol. 97, Issue 1
1 Jan 2020
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Research ArticleArticle

HSP90 regulation of P2X7 receptor function requires an intact cytoplasmic C-terminus

Keisuke Migita, Taku Ozaki, shuji Shimoyama, Junko Yamada, Yoshikazu Nikaido, Tomonori Furukawa, Yuko Shiba, Terrance M Egan and Shinya Ueno
Molecular Pharmacology June 14, 2016, mol.115.102988; DOI: https://doi.org/10.1124/mol.115.102988

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Research ArticleArticle

HSP90 regulation of P2X7 receptor function requires an intact cytoplasmic C-terminus

Keisuke Migita, Taku Ozaki, shuji Shimoyama, Junko Yamada, Yoshikazu Nikaido, Tomonori Furukawa, Yuko Shiba, Terrance M Egan and Shinya Ueno
Molecular Pharmacology June 14, 2016, mol.115.102988; DOI: https://doi.org/10.1124/mol.115.102988
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