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Molecular Pharmacology

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Research ArticleArticle

Targeting a PAR4 carboxyl terminal motif to regulate platelet function

Rithwik Ramachandran, Koichiro Mihara, Pierre Thibeault, Christina M Vanderboor, Bjoern Petri, Mahmoud Saifeddine, Michel Bouvier and Morley D. Hollenberg
Molecular Pharmacology January 26, 2017, mol.116.106526; DOI: https://doi.org/10.1124/mol.116.106526
Rithwik Ramachandran
University of Western Ontario;
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Koichiro Mihara
University of Calgary;
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Pierre Thibeault
University of Western Ontario;
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Christina M Vanderboor
University of Western Ontario;
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Bjoern Petri
University of Calgary;
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Mahmoud Saifeddine
University of Calgary;
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Michel Bouvier
University of Montreal;
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Morley D. Hollenberg
The University of Calgary
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Abstract

Thrombin initiates human platelet aggregation by coordinately activating proteinase activated receptors (PARs)-1 and -4. However, targeting PAR1 with an orthostatic tethered ligand binding site antagonist results in bleeding, possibly due to the important role of PAR1 activation on cells other than platelets. Because of its more restricted tissue expression profile, we have therefore turned to PAR4 as an antiplatelet target. We have identified an intracellular PAR4 C-terminal motif that regulates calcium signaling and β-arrestin interactions. By disrupting this PAR4 calcium/β-arrestin signaling process with a novel cell-penetrating peptide, we are able to inhibit both thrombin-triggered platelet aggregation in-vitro and clot consolidation in-vivo. We suggest that targeting PAR4 represents an attractive alternative to blocking PAR1 for antiplatelet therapy in humans.

  • Thrombin
  • AKT / PKB
  • Platelets
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 97 (1)
Molecular Pharmacology
Vol. 97, Issue 1
1 Jan 2020
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Research ArticleArticle

Targeting a PAR4 carboxyl terminal motif to regulate platelet function

Rithwik Ramachandran, Koichiro Mihara, Pierre Thibeault, Christina M Vanderboor, Bjoern Petri, Mahmoud Saifeddine, Michel Bouvier and Morley D. Hollenberg
Molecular Pharmacology January 26, 2017, mol.116.106526; DOI: https://doi.org/10.1124/mol.116.106526

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Research ArticleArticle

Targeting a PAR4 carboxyl terminal motif to regulate platelet function

Rithwik Ramachandran, Koichiro Mihara, Pierre Thibeault, Christina M Vanderboor, Bjoern Petri, Mahmoud Saifeddine, Michel Bouvier and Morley D. Hollenberg
Molecular Pharmacology January 26, 2017, mol.116.106526; DOI: https://doi.org/10.1124/mol.116.106526
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