Article Figures & Data
Additional Files
Data Supplement
- Supplemental Data -
Supplemental Methods
Supplemental Figure 1 - Verification of laboratory-prepared glut-DNICs by optical and EPR methods
Supplemental Figure 2 - Maximal tension of sheep mesenteric arterial rings in the absence and presence of different test compounds, including 2% (v/v) plasma, 15 μM albumin, 1000U/ml SOD, 200 μM CPTIO, 1000U/ml SOD+200 μM CPTIO, or 1mM DTT (n≥4; p=0.7930; one-way ANOVA)
Supplemental Figure 3 - Role of HNO in glut-MDNIC-mediated relaxation of isolated sheep mesenteric arteries (n≥4)
Supplemental Figure 4 - In vitro measurements seeking to detect NO⋅ release from glut-BDNIC
Supplemental Figure 5 - Effects of plasma on relaxation by the HNO donor AS (n=4)
Supplemental Figure 6 - Effects of plasma (2% v/v) and albumin (15μM) on glut-MDNIC-mediated relaxation (n=6)
Supplemental Figure 7 - EPR spectrum (150 K) of metabolites of 25μM glut-BDNIC and 50μM glut-MDNIC in (A) plasma, (B) blood, and (C) albumin solutions in vitro
Supplemental Figure 8 - Representative, room temperature EPR spectra of glut-BDNIC following addition to whole blood, plasma, and albumin solution, as well as that of low molecular weight glut-MDNIC standard (N15 labeled)
References
- Supplemental Data -