Analysis of the dissociated steroid, RU24858, does not exclude a role for inducible genes in the anti-inflammatory actions of glucocorticoids

Data Supplement

Files in this Data Supplement:

• Data Supplement - This supplement contains:

  Supplemental Experimental Procedures

  Supplemental Results

  Supplemental References

  Supplemental Figures:

  Supplemental Fig. 1. Relationship between starting cDNA concentration and amplification product using semi-quantitative RT-PCR and real-time TaqMan PCR.
  Supplemental Fig. 2. Activation and relative GR affinities for dexamethasone and RU24858.
  Supplemental Fig. 3. Repression of COX-2 and IL-8 mRNA expression by dexamethasone and RU24858 is antagonized by RU486.
  Supplemental Fig. 4. Effect of dexamethasone on wild-type and transfected cell lines.
  Supplemental Fig. 5. TaqMan real-time RT-PCR analysis of unspliced IL-8 and GAPDH nuclear RNA.
  Supplemental Fig. 6. Real-time RT-PCR analysis to show that repression of COX-2 and IL-8 mRNA by dexamethasone and RU24858 is prevented by actinomycin D.