MiTMAB and OcTMAB are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II
Data Supplement
Files in this Data Supplement:
- Data Supplement - Figure 1. Cells were serumstarved for 2 h at 37EC, after which they were treated with 30µn;M MiTMAB or 30µn;M myristic acid for 10 min, and incubated a further 20 min in the presence of Tf-A594 or EGFTxR as an internal positive controls that the drugs worked and that the DNA construct was not inhibitory.
- Data Supplement - Figure 2. MiTMAB also blocked its localization to the plasma membrane in HeLa cells and myristic acid was without effect.
- Data Supplement - Movie 1. In vehicle treated control cells EGF-A488 bound to the cell surface and was not internalized at 4�C.
- Data Supplement - Movie 2. ... distribution of the cell surface binding was altered by MiTMAB treatment, resulting in increased localization of the EGFR at cell-cell contacts.
