Alternative translation initiation of human RGS2 yields a set of functionally distinct proteins

Data Supplement

Files in this Data Supplement:

• Data Supplement - Supplemental Fig. 1. Doxycycline induced expression profiles of NT(WT)-GFP.
  A. Expression profile of NT(WT)-GFP in the presence of 5mg/mL doxycycline in the presence and absence of activated Gs and ACV. Tet-ON HEK293 cells were incubated for 48 hours post transfection in 5mg/mL doxycycline before harvesting and separation on a SDS-polyacrylimide gel as described in methods.
  B. Expression profile of NT(WT)-GFP in varying concentrations of doxycycline. Tet-ON HEK293 cells were incubated for 48 hours post transfection with the indicated concentrations of doxycycline before harvesting and separation as described.