Agonist-promoted Lys 63-linked polyubiquitination of the human kappa opioid receptor is involved in receptor down-regulation

Data Supplement

Files in this Data Supplement:

• Data Supplement - Supplemental Data 1. Basal ubiquitination of the hKOR was not affected by dominant negative mutants of GRK2, β-arrestin and dynamin I.
• Data Supplement - Supplemental Data 2. Pretreatment with the lysosomal inhibitor chloroquine reduced Dyn Ainduced down-regulation of the wildtype and 10KR mutant of the hKOR.
• Data Supplement - Supplemental Data 3 Time course of recycling of the wildtype hKOR and 10KR mutant.