Ethanol modulates BKCa channels by acting as an adjuvant of calcium
Data Supplement
Files in this Data Supplement:
- Data Supplement - Starting matrix, rate constants, and channel state dwell probabilities corresponding to the kinetic schemes shown in Figs. 7A-D...
- Data Supplement - Complete Figure Legends
- Data Supplement - Fig. S1. Fitting of macroscopic current data to a Monod-Wymann-Changeux (MWC), 10-state model indicates that ethanol modulates BKCa currents by changing the Ca2+i-dependent parameters.
- Data Supplement - Fig. S2. Ethanol dual modulation of mslo channel steady-state activity (NPo) occurs without changes in major ion conduction properties of the channel pore.
- Data Supplement - Fig. S3. Computer simulated single channel data obtained from running the empiric kinetic model shown in Fig. 4.
- Data Supplement - Fig. S4. In the presence of BKCa &beta4 subunits, ethanol still evokes dual responses in mslo channel activity as a function of Ca2+i.
- Data Supplement - Fig. S5. The D362A/D367A mutations abolish both Ca2+i-driven entry into the low-activity mode and ethanol-inhibition of activity at high Ca2+i.
- Data Supplement - Fig. S6. Even when Ca2+i is raised to 1 mM, the combined 5D5N, D362A/D367A mutant channel is not activated by ethanol.
- Data Supplement - Fig. S7. Computational models for calcium and ethanol interactions with the slo channel high affinity metal recognition sites based on crystal structures of calmodulin.
