Dynamic Effect of Bortezomib on NF-κB Activity and Gene Expression in Tumor Cells

Data Supplement

Files in this Data Supplement:

• Data Supplement -
  Figure S1. The number-labeled cells (shown at t = 0) were used to produce the quantified data in Figure 1B and Figure S2...
  Figure S2. Quantification of cells 5 to 12 shown in Figure S1 in the same format as in Figure 1B...
  Figure S3. The labeled cells (shown at t =0) were used to produce the quantified data in Figure 3B...
  Figure S4. Immunofluorescence to measure the p65-EGFP fusion level in comparison to the total p65...
  
• Data Supplement - Movie S1. Time course of cells expressing p65:EGFP (green) and pIL-8:mCherry (red) after TNF-alpha treatment, quantified in Figure 1B.
• Data Supplement - Movie S2. Time course of TNF-alpha pre-treated cells expressing p65:EGFP (green) and pIL-8:mCherry (red) after bortezomib treatment, quantified in Figure 3B.
• Data Supplement - Movie S3. Time course of a single living cell expressing p65:EGFP after TNF-alpha treatment, represented by both the grayscale time lapse images and the corresponding quantification of nuclear:total ratio from EGFP intensities. The time lapse image acquisition started 15 minutes after TNF-alpha treatment. The graph in the plot tracks the nuclear p65 level of the cell on the left side of the field of view.