STI571-Induced Cell Edge Translocation of Kinase-Active and Kinase-Defective Abl: Requirements of Myristoylation and SH3 Domain

Data Supplement

Files in this Data Supplement:

• Data Supplement -
  Complete movie legends.
  Figure 1. Dynamic behaviour of abl-EGFP in XTC fibroblasts...
  Figure 2. F-actin binding is dispensable for STI571-induced translocation of abl...
• Data Supplement - Movie 1. Induction of finger-shaped actin structures by overexpression of abl-EGFP in XTC cell
• Data Supplement - Movie 2. Translocation of abl-EGFP to the lamellipodium tip as well as at the punctate actin rich foci induced by STI571
• Data Supplement - Movie 3. Cell edge translocation of abl is not due to redistribution of F-acti
• Data Supplement - Movie 4. Translocation of abl-KD-EGFP, a kinase-dead mutant of abl, to the lamellipodium tip as well as at the punctate actin rich foci induced by STI571..
• Data Supplement - Movie 5. Dose-response analysis of STI571-induced cell edge translocation of abl-KD using a computer-assisted method..
• Data Supplement - Movie 6. Adverse effects of STI571 on the cell edge association between the STI571-resistant abl-T334I and its wild-type variant...
• Data Supplement - Movie 7. N-myristoylation is essential in STI571-induced cell edge translocation of abl...
• Data Supplement - Movie 8. Constitutive association to the lamellipodium tip of the abl-A356N mutant defective in the intramolecular interaction of the myristoyl group with the kinase C-lob...
• Data Supplement - Movie 9.Constitutive association to the lamellipodium tip of the abl-P242E/P249E (abl-PP) mutant defective in the intramolecular interaction between the SH3 domain and the SH2-kinase linke
• Data Supplement - Movie 10. Cell edge translocation of abl-�FB defective in F-actin binding induced by STI571...
• Data Supplement - Movie 11. Further deletion of the C-terminus which had eliminated the entire last exon region weakened STI571-induced translocation of abl...
• Data Supplement - Movie 12. Two different exposure times to distinguish the fast and the slow diffusive species of abl visualized at the single-molecule level...
• Data Supplement - Movie 13. Slow diffusive motion of abl-KD visualized as single-molecules at the cell periphery...